FISH, a new tool for in situ preservation of RNA in tissues of deep‐sea mobile fauna DOI Creative Commons
Valérie Cueff‐Gauchard, Johanne Aubé,

Jean‐Romain Lagadec

et al.

Limnology and Oceanography Methods, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

ABSTRACT Accessing the metabolic functioning of deep‐sea animals in situ remains a technological challenge as recovery time samples is incompatible with short lifespan such molecules mRNAs. Tools able to preserve RNA exist, but they are study mobile fauna. Here, we describe new sampling tool, named FISH (fixer homogenized substrates), implemented on submersible and equipped number specific features collect tissue Connected suction pump submersible, sampler incorporates bowl which two bottles preservative reagent attached, hose, support containing motor connected by magnetic coupling system. We used hydrothermal shrimp Rimicaris exoculata from Mid‐Atlantic Ridge model test suitability our tool. The was compared other methods, use metatranscriptomic approach targeting microbial communities associated cephalothorax symbionts. quality, gene assignment, taxonomic function diversity showed differences between on‐board preservation tissues. Of alternative methods tested, clearly not suitable for RNA‐based studies, while pressurized results closer sample quality obtained sampling. has therefore demonstrated be cost‐effective reliable tool efficiently recovered environments.

Language: Английский

FISH, a new tool for in situ preservation of RNA in tissues of deep‐sea mobile fauna DOI Creative Commons
Valérie Cueff‐Gauchard, Johanne Aubé,

Jean‐Romain Lagadec

et al.

Limnology and Oceanography Methods, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

ABSTRACT Accessing the metabolic functioning of deep‐sea animals in situ remains a technological challenge as recovery time samples is incompatible with short lifespan such molecules mRNAs. Tools able to preserve RNA exist, but they are study mobile fauna. Here, we describe new sampling tool, named FISH (fixer homogenized substrates), implemented on submersible and equipped number specific features collect tissue Connected suction pump submersible, sampler incorporates bowl which two bottles preservative reagent attached, hose, support containing motor connected by magnetic coupling system. We used hydrothermal shrimp Rimicaris exoculata from Mid‐Atlantic Ridge model test suitability our tool. The was compared other methods, use metatranscriptomic approach targeting microbial communities associated cephalothorax symbionts. quality, gene assignment, taxonomic function diversity showed differences between on‐board preservation tissues. Of alternative methods tested, clearly not suitable for RNA‐based studies, while pressurized results closer sample quality obtained sampling. has therefore demonstrated be cost‐effective reliable tool efficiently recovered environments.

Language: Английский

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