Selection of suitable reference lncRNAs for gene expression analysis in Osmanthus fragrans under abiotic stresses, hormone treatments, and metal ion treatments

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 21, 2025

Intoduction Osmanthus fragrans , a well-regarded traditional flower in China, holds extensive applications gardening, food, cosmetics, and Chinese medicine. Despite its importance, research on long non-coding RNAs (lncRNAs) O. has been constrained by the absence of reliable reference genes (RGs). Methods We employed five distinct algorithms, i.e., delta-Ct, NormFinder, geNorm, BestKeeper, RefFinder, to evaluate expression stability 17 candidate RGs across various experimental conditions. Results discussion The results indicated most stable RG combinations under different conditions as follows: cold stress: lnc00249739 lnc00042194; drought lnc00042194 lnc00174850; salt lnc00239991 abiotic lnc00239991, lnc00042194, lnc00067193, lnc00265419; ABA treatment: 18S ; MeJA lnc00265419 lnc00249739; ethephon lnc00229717 lnc00044331; hormone treatments: lnc00239991; Al 3+ lnc00087780 Cu 2+ lnc00067193 Fe ACT7 metal ion lnc00067193; flowering stage: RAN1 tissues: TUA5 UBQ4 all samples: . reliability these selected was further validated analyzing patterns lnc00003036, lnc00126603, lnc00250780. This study represents first comprehensive evaluation lncRNA significantly enhancing accuracy depth analyses this species, contributing advancements plant stress resistance breeding improving environmental adaptability.

Language: Английский

Projecting the global potential distribution of nine Rhododendron Subgenus Hymenanthes species under different climate change scenarios

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 27, 2025

As one of China's most treasured traditional flowers, Rhododendron Subgen. Hymenanthes is renowned worldwide for its evergreen foliage, vibrant and significant ornamental, landscaping, economic value. However, climate change poses a serious threat to future, leading population declines endangerment some species. Despite the ecological importance Hymenanthes, future distribution suitable habitats effective strategies conservation utilization remain unclear. This study employs MaxEnt model, which well-known reliability in predicting species under changing environmental conditions, predict potential global nine Hymenanthes. The goal provide solid foundation their conservation, cultivation management, breeding. results indicate that, scenarios, habitat areas four (R. irroratum, R. agastum, decorum, arboreum) will significantly decrease, while remaining five delavayi, fortunei, calophytum, simiarum, wardii) experience slight expansion. Temperature precipitation are identified as key factors influencing growth these species, affecting ability colonize new regions. migration direction expanding regions all consistent, with centroids shifting towards northwest. These findings critical insights developing targeted strategies, including identifying refugia prioritizing conditions.

Language: Английский

A Simulation of a Suitable Habitat for Acer yangbiense and Cinnamomum chago Under Climate Change

Forests, Journal Year: 2025, Volume and Issue: 16(4), P. 621 - 621

Published: April 2, 2025

Species migration or extinction events may occur on a large scale with the intensification of climate change. Plant Extremely Small Populations (PSESP) are more sensitive to change as compared other plants. To date, potential effect Acer yangbiense and Cinnamomum chago, both which belong PSESP, remain unknown. In this study, we modeled distribution dynamics A. C. chago spanning from Last Glacial Maximum (LGM) end 21st century based MaxEnt model, optimized using Kuenm package. The results revealed that parameter settings optimal models were RM (regularization multiplier) = 3.5, FC (feature combination) QP, 2, QPT. had AUCs 0.982 0.993, respectively, indicating model predictions highly accurate while effectively balancing complexity avoiding overfitting. was mostly influenced by precipitation driest quarter (bio17) min temperature coldest month (bio6). From LGM present, total suitable areas initially declined before showing subsequent increase, but it is projected experience significant reductions in future, decreases 32.98%–64.99% 63.48%–99.49%, respectively. centroids showed trend south north expected continue. enhance resilience meet challenges proposed introduction artificial cultivation these species should be carried out Baoshan, Dali, Nujiang northwest Yunnan Province, high heat values, so expand populations gradually.

Language: Английский