Cynthichlorine Extracted from Ascidian Cynthia savignyi from Djibouti: Optimization of Extraction, In Vitro Anticancer Profiling, and In Silico Approach DOI Creative Commons
Fatouma Mohamed Abdoul‐Latif,

Houda Mohamed,

Ibrahim Houmed Aboubaker

et al.

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(4), P. 172 - 172

Published: April 16, 2025

This work focuses on the extraction of cynthichlorine from ascidian Cynthia savignyi, a molecule that has potential promise as an anticancer agent. The main objective was to optimize conditions and evaluate cytotoxic activity in tumor cell lines. Two methods, maceration Soxhlet extraction, were compared, with showing significantly higher yield (2.2 ± 0.2%) compared (1.0 0.2%). An optimization factors influencing performed using Box–Behnken method, temperature time have negative impact yield, optimal being below 25 °C those 12 h. Cytotoxic assessment revealed marked inhibition growth all tested lines (U87-MG, U2OS, NCI-N87, HCT116, A2780), IC50 values ranging 0.162 µg/mL U87-MG 0.576 NCI-N87. Finally, computational analysis showed exhibits high electronic stability notable affinity for some biological targets, including NM23-H2, suggesting its targeted therapy cancer treatment. These results pave way future studies therapeutic use cynthichlorine.

Language: Английский

Cynthichlorine Extracted from Ascidian Cynthia savignyi from Djibouti: Optimization of Extraction, In Vitro Anticancer Profiling, and In Silico Approach DOI Creative Commons
Fatouma Mohamed Abdoul‐Latif,

Houda Mohamed,

Ibrahim Houmed Aboubaker

et al.

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(4), P. 172 - 172

Published: April 16, 2025

This work focuses on the extraction of cynthichlorine from ascidian Cynthia savignyi, a molecule that has potential promise as an anticancer agent. The main objective was to optimize conditions and evaluate cytotoxic activity in tumor cell lines. Two methods, maceration Soxhlet extraction, were compared, with showing significantly higher yield (2.2 ± 0.2%) compared (1.0 0.2%). An optimization factors influencing performed using Box–Behnken method, temperature time have negative impact yield, optimal being below 25 °C those 12 h. Cytotoxic assessment revealed marked inhibition growth all tested lines (U87-MG, U2OS, NCI-N87, HCT116, A2780), IC50 values ranging 0.162 µg/mL U87-MG 0.576 NCI-N87. Finally, computational analysis showed exhibits high electronic stability notable affinity for some biological targets, including NM23-H2, suggesting its targeted therapy cancer treatment. These results pave way future studies therapeutic use cynthichlorine.

Language: Английский

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