Comparative analysis of five mitochondrial genomes of the subfamily Galerucinae (Coleoptera: Chrysomelidae) and evolution of control regions inferred from phylogeny DOI Creative Commons
Shiwen Xu, Pu Miao, Qiaoqiao Liu

et al.

Frontiers in Ecology and Evolution, Journal Year: 2025, Volume and Issue: 13

Published: April 28, 2025

Introduction Mitochondrial genome (mitogenome) is a widely utilized source of genetic data for animal species identification, molecular phylogeny, phylogeography, and evolutionary research at various taxonomic scales. With 14,500 recognized placed in over 1,100 genera, Galerucinae (Coleoptera: Chrysomelidae), also known as leaf beetles, the largest subfamily within Chrysomelidae. Methods We newly sequenced annotated five mitogenomes from Galerucinae. Additionally, we investigated their nucleotide composition, codon usage, relative synonymous usage rate protein-coding genes (PCGs). The phylogenetic relationship been inferred by gathering 97 GenBank our mitogenomes. divergence times were estimated using three calibration nodes MCMCTree. Furthermore, structural features control regions (CRs) framework Results discussion length ranged 15,737 to 16,484 bp. All these exhibited high AT content, well positive skew negative GC skew. Most PCGs have ATN start codons, TAA TAG most assigned stop codons. substitution analysis suggested that ATP8 experienced selection, while COX1 underwent purifying selection. Phylogenetic analyses showed both tribes Alticini Galerucini monophyletic sister groups. Within two tribes, groups subtribes monophyletic. Our time inference, dating origin Middle Cretaceous period (102.69 Ma), indicated main diverged occurred during Paleogene. Finally, revealed variations organization CRs even among closely related groups, which can be attributed specific elements.

Language: Английский

Comparative analysis of five mitochondrial genomes of the subfamily Galerucinae (Coleoptera: Chrysomelidae) and evolution of control regions inferred from phylogeny DOI Creative Commons
Shiwen Xu, Pu Miao, Qiaoqiao Liu

et al.

Frontiers in Ecology and Evolution, Journal Year: 2025, Volume and Issue: 13

Published: April 28, 2025

Introduction Mitochondrial genome (mitogenome) is a widely utilized source of genetic data for animal species identification, molecular phylogeny, phylogeography, and evolutionary research at various taxonomic scales. With 14,500 recognized placed in over 1,100 genera, Galerucinae (Coleoptera: Chrysomelidae), also known as leaf beetles, the largest subfamily within Chrysomelidae. Methods We newly sequenced annotated five mitogenomes from Galerucinae. Additionally, we investigated their nucleotide composition, codon usage, relative synonymous usage rate protein-coding genes (PCGs). The phylogenetic relationship been inferred by gathering 97 GenBank our mitogenomes. divergence times were estimated using three calibration nodes MCMCTree. Furthermore, structural features control regions (CRs) framework Results discussion length ranged 15,737 to 16,484 bp. All these exhibited high AT content, well positive skew negative GC skew. Most PCGs have ATN start codons, TAA TAG most assigned stop codons. substitution analysis suggested that ATP8 experienced selection, while COX1 underwent purifying selection. Phylogenetic analyses showed both tribes Alticini Galerucini monophyletic sister groups. Within two tribes, groups subtribes monophyletic. Our time inference, dating origin Middle Cretaceous period (102.69 Ma), indicated main diverged occurred during Paleogene. Finally, revealed variations organization CRs even among closely related groups, which can be attributed specific elements.

Language: Английский

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