Past population control biases interpretations of contemporary genetic data: implications for future invasive Sitka black-tailed deer management in Haida Gwaii

Biological Invasions, Journal Year: 2023, Volume and Issue: 25(12), P. 3871 - 3886

Published: Aug. 12, 2023

Language: Английский

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Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 13, 2024

Abstract Populations in isolated and small fragments lose genetic variability very fast are usually of conservation concern because they at greater risk local extinction. The largest native deer South America, Blastocerus dichotomus (Illiger, 1815) , is a Vulnerable species according to the IUCN categorization, which inhabits tropical subtropical swampy areas. In Argentina, its presence has been restricted four fragments. Here we examine diversity differentiation among three them, including different patches that form southernmost population, using 18 microsatellite markers genotyped by Amplicon Sequencing DNA extracted from fecal samples. Genetic was low (H E < 0.45) all populations studied. We found clusters compatible with geographic location also metapopulation dynamics involves make up evidence barrier gene flow between two them. Our results point creation corridor as necessary urgent management action. This first study, population level, employing genotyping non-invasive samples an endangered species.

Language: Английский

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FecalSeq enrichment with RAD Sequencing from non-invasive environmental samples holds promise for genetic monitoring of an imperiled lagomorph

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 30, 2024

Despite advances in genomic sequencing and bioinformatics, conservation genomics is still often hindered by a reliance on non-invasive samples. The presence of exogenous DNA the low quantity poor quality samples have been roadblock to sequencing, thereby limiting potential for monitoring endangered species. Recent molecular advances, such as host enrichment, hold promise facilitating from We used FecalSeq method enrich extracted wild-collected fecal pellets imperiled New England cottontail identified SNPs 3RAD Sequencing. obtained rabbit pellets, including that were collected environmental conditions performed poorly with microsatellites. Measures success improved greater amounts starting 32% generated SNP genotypes passed control filtering. Genotyping error rates high, however, approach was unable consistently distinguish unique individuals or matching genotypes, while it suitable recovering expected population structure. Pairing enrichment RADseq promising low-cost wild populations using an context, but may be better suited informing through genomics.

Language: Английский

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From single species to communities: microsatellite amplicon sequencing to monitor felids using Feliplex

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract The current biodiversity crisis demands a shift from single-species to multispecies approaches in conservation, particularly for rare and endangered species. However, this transition requires tools optimised research, which are currently limited. Recent advances high-throughput sequencing (HTS) technologies bioinformatics have enabled efficient robust acquisition of genetic data. Amplicon approaches, particular, demonstrated potential enhancing non-invasive studies species, but their application has been mostly limited single To enable we introduce cost-effective HTS-based amplicon approach genotyping multiple species simultaneously, designed population monitoring, including individual identification ascertaining patterns structure. We developed Feliplex, multiplex panel 85 co-amplifying tetranucleotide microsatellite markers cross-genotyping Felidae demonstrate the utility our approach. Feliplex was validated on known samples nine Indian felid across genera Panthera, Prionailurus , Felis . applied it invasive (blood tissue) (hair faeces) DNA extracts 173 wild individuals obtaining respectively 70% 56% multilocus success rates. accurately identified clusters tigers ( Panthera tigris n=19) revealed hitherto unknown structure fishing cats viverrinus n=40). Feliplex’s wide applicability allows reliable low-quality/quantity samples, while supporting conservation monitoring lesser-known like small cats. Our broad can be adapted develop similar panels closely related groups.

Language: Английский

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