Molecular Ecology of Freshwater Turtles and Future directions DOI Creative Commons

Iqra Mushtaq Mushtaq

Agricultural Sciences Journal, Journal Year: 2022, Volume and Issue: 4(2), P. 115 - 126

Published: Dec. 31, 2022

The distribution and diversity of turtles now reflect the lengthy complex evolution taxonomy, which represents an old group tetrapod vertebrates in terms evolutionary history. Freshwater represent majority 365 species, they mostly live tropical subtropical regions. Emydidae hotspots can be found Southeast North America, as Geoemydidae Trionychidae Indo-Malayan area. While Pelomedusidae are Africa, Chelidae primarily Neotropics Australia. Most species genus endemic to a particular region or even single location. freshwater suffer varied degrees threat, from habitat changes collection. With use morphological molecular data, phylogenetic trees for different turtle have been generated using DNA techniques procedures. complete mitochondrial (mtDNA), dehydrogenase subunit 4 (ND4), cytochrome b (Cyt b), carapacial ridge (CR), c oxidase I (CO I) genes were sequenced by universal PCR long-PCR methods. Along with CR sequences turtles, composition structure control diverse compared analysed. Functional domains regulatory area, well their conserved sequences, determined based on sequence similarities other turtles. regions flanking recovered Long-PCR gene-specific primers. To clarify genetic links between fresh water that share same type, tree was created Cytochrome sequencing data PCR- Restriction fragment length polymorphism (RFLP) pattern. Keywords: Complex evolution, Phylogenetics, Phylogenomics, Tetrapod

Language: Английский

New insights on sea turtle behaviour during the ‘lost years’ DOI Creative Commons
Katrina F. Phillips, Nathan F. Putman, Katherine L. Mansfield

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2025, Volume and Issue: 292(2040)

Published: Feb. 1, 2025

Several marine turtle species spend their first years of life in oceanic habitats. This early stage is referred to as the ‘lost years’ due difficulty accessing individuals for study offshore. We satellite tracked 114 wild-caught juvenile turtles (straight carapace lengths 12.3–29.9 cm) from Gulf Mexico between 2011 and 2022 investigate movements with respect traditional definitions assigned stage. Satellite-tracked included 79 green ( Chelonia mydas ), 26 Kemp’s ridleys Lepidochelys kempii 5 loggerheads Caretta caretta ) 4 hawksbills Eretmochelys imbricata ). Many transited (>200 m depth) neritic waters (<200 depth), challenging assumption that this exclusively found Turtle differed oceanographic surface drifters, providing further evidence sea do not drift currents. recommend redefining ‘oceanic stage’ a ‘dispersal better reflect behaviour habitat plasticity. Our findings establish West Florida Shelf high-use area, particularly among ridleys. The northeastern an important region these conservation concern.

Language: Английский

Citations

1
Genomic insights into marine environment adaptation and conservation of the threatened olive ridley turtle (Lepidochelys olivacea) DOI Creative Commons
Lin Yang, Yiting Chen,

Shaofeng Wang

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(2), P. 111776 - 111776

Published: Jan. 8, 2025

Sea turtles are marine flagship species and most of them currently in a threatened state. Long-term surviving the ocean has driven significant morphological physiological changes for this group, which makes an ideal model studying adaptive evolution environments. Herein, we present chromosome-scale genome Lepidochelys olivacea with size 2.22 Gb contig N50 97.3 Mb. Comparative genomic analyses uncovered suite genes related to olfaction, vision, virus defense, longevity, may help explain genetic underpinnings its environment adaptation. We also observed that genome-wide heterozygosity L. was low (6.45e-4), consistent prolonged population decline. Overall, our study provides valuable resources understanding evolutionary adaptations aquatic conservation species.

Language: Английский

Citations

0
Genetic variation among sea turtle life stages and species suggests connectivity among ocean basins DOI Creative Commons
Katrina F. Phillips, Katherine R. Martin, Gustavo D. Stahelin

et al.

Ecology and Evolution, Journal Year: 2022, Volume and Issue: 12(11)

Published: Oct. 30, 2022

Abstract Regional genetic differentiation of mitochondrial lineages occurs in migratory species with natal philopatry such as sea turtles. However, early juvenile dispersal represents a key opportunity for gene flow and colonization new regions through founder events, making it an important yet under‐studied life stage. To assess connectivity among turtle stages ocean basins, we sequenced DNA (mtDNA) fragments from 35 juveniles sampled the Gulf Mexico rarely observed stage across three species: green turtles ( Chelonia mydas ; n = 30), hawksbills Eretmochelys imbricata 3), loggerheads Caretta caretta 2). We estimated rookery contributions using many‐to‐many Bayesian mixed stock analysis that incorporated probabilities based on size transport via currents. assembled tree including 709 distinct mtDNA control region haplotypes literature all seven extant to gaps life‐stage data well contextualize dispersing juveniles. Our results indicate high likelihood originated rookeries along coast Mexico, smaller Costa Rica Suriname. The yielded species‐level relationships consistent those presented previously, while intra‐species between basins differed, particularly within loggerhead clades. highlight lack turtles, especially stage, potential these answer broader questions diversification lineages.

Language: Английский

Citations

6
First molecular report of the Chelonid alphaherpesvirus 5 (ChHV5 Scutavirus chelonidalpha5) in a green turtle (Chelonia mydas) with fibropapillomatosis in the southwest Gulf of Mexico DOI Creative Commons

Joelly Espinoza,

Eduardo Gazol,

Maurilia Rojas-Contreras

et al.

European Journal of Wildlife Research, Journal Year: 2024, Volume and Issue: 70(6)

Published: Dec. 1, 2024

Abstract Fibropapillomatosis (FP) is an emerging neoplastic disease associated with chelonid herpesvirus 5 (ChHV5; Scutavirus chelonidalpha ) that affects all species of marine turtles worldwide, mainly green ( Chelonia mydas at coastal feeding sites. This report describes the case a juvenile turtle stranded alive on coast Veracruz, Mexico presented 41 lesions suggestive FP distributed eyes, neck, front flippers, axillary/inguinal regions and plastron. Morphologically, varied in size, shape appearance surface. A tumour was collected analysed by histopathology revealing benign neoplasm fibropapilloma characteristics (dermal epidermal proliferation) cytopathic effects consistent infection, such as ballooning, reticular, vacuolar degeneration, cell necrosis, eosinophilic intranuclear inclusion bodies, inflammatory infiltration. The tested positive for ChHV5 through conventional PCR targeting UL30, UL18, UL22, UL27 genes. Phylogenetic analysis DNA Polymerase (UL30) placed Veracruz variant Western Atlantic/Eastern Caribbean cluster along sequences from Florida, Colombia, Barbados, Brazil. Additional identification CMA1.1 mitochondrial haplotype this individual supports connectivity between northern southern Gulf (GoM) Caribbean. It also suggests potential risk route infection. details first linked to southwestern GoM. Further research these areas crucial due their role habitats five sea across various life stages.

Language: Английский

Citations

0
Unraveling migratory corridors of loggerhead and green turtles from the Yucatán Peninsula and its overlap with bycatch zones of the Northwest Atlantic DOI Creative Commons
Elizabeth Labastida‐Estrada, Salima Machkour‐M’Rabet

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0313685 - e0313685

Published: Dec. 6, 2024

Bycatch represents a conservation problem when endangered species are affected. Sea turtles highly vulnerable to this threat as their critical habitats overlap with fishing zones in all regions of the world. We used sequences mitochondrial DNA control region obtained from loggerhead ( Caretta caretta ) and green Chelonia mydas determine migratory routes between nesting Yucatán Peninsula marine Northwest Atlantic. Mixed Stock Analysis revealed that loggerheads Quintana Roo migrated foraging areas northwestern Migratory by determined natal colony: (1) Gulf Mexico migrate aggregations Texas northern Mexico, (2) Mexican Caribbean travel grounds Florida, (3) smaller proportion individuals born display local connectivity pattern. Our results suggest corridors longline fisheries mid-Atlantic where sea turtle bycatch is comprised predominantly immature individuals. Green shrimp trawl within Mexico. data identification suggests on east coast U.S. pose serious recovery populations.

Language: Английский

Citations

0
Molecular Ecology of Freshwater Turtles and Future directions DOI Creative Commons

Iqra Mushtaq Mushtaq

Agricultural Sciences Journal, Journal Year: 2022, Volume and Issue: 4(2), P. 115 - 126

Published: Dec. 31, 2022

The distribution and diversity of turtles now reflect the lengthy complex evolution taxonomy, which represents an old group tetrapod vertebrates in terms evolutionary history. Freshwater represent majority 365 species, they mostly live tropical subtropical regions. Emydidae hotspots can be found Southeast North America, as Geoemydidae Trionychidae Indo-Malayan area. While Pelomedusidae are Africa, Chelidae primarily Neotropics Australia. Most species genus endemic to a particular region or even single location. freshwater suffer varied degrees threat, from habitat changes collection. With use morphological molecular data, phylogenetic trees for different turtle have been generated using DNA techniques procedures. complete mitochondrial (mtDNA), dehydrogenase subunit 4 (ND4), cytochrome b (Cyt b), carapacial ridge (CR), c oxidase I (CO I) genes were sequenced by universal PCR long-PCR methods. Along with CR sequences turtles, composition structure control diverse compared analysed. Functional domains regulatory area, well their conserved sequences, determined based on sequence similarities other turtles. regions flanking recovered Long-PCR gene-specific primers. To clarify genetic links between fresh water that share same type, tree was created Cytochrome sequencing data PCR- Restriction fragment length polymorphism (RFLP) pattern. Keywords: Complex evolution, Phylogenetics, Phylogenomics, Tetrapod

Language: Английский

Citations

1