The geographic and phylogenetic structure of public DNA barcode databases: an assessment using Chrysomelidae (leaf beetles)

Frontiers in Ecology and Evolution, Journal Year: 2024, Volume and Issue: 12

Published: May 1, 2024

Introduction DNA barcoding in insects has progressed rapidly, with the ultimate goal of a complete inventory world’s species. However, effort to date been driven by few national campaigns and leaves much world unsampled. This study investigates what degree current barcode data cover species diversity across globe, using leaf beetle family Chrysomelidae as an example. Methods A recent version (June 2023) Barcode-of-Life database was subjected test sampling completeness barcode-to-BIN ratio coverage (SC) metric. All barcodes were placed phylogenetic tree ~600 mitochondrial genomes, applying (PD) metrics community phylogenetics sets for at clade level reveal global structure diversity. Results The included 73342 barcodes, grouped into 5310 BINs (species proxies) from 101 countries. Costa Rica contributed nearly half all sequences, while 50 countries represented less than ten barcodes. Only five countries, Rica, Canada, South Africa, Germany, Spain, had high completeness, although collectively covers most major taxonomic biogeographically confined lineages. PD showed moderate saturation more is added country, indicated clustering faunas. remained incomplete even intensely sampled insufficient assessment richness patterns. Discussion sequence-based needs be greatly expanded include areas deeper local before reaching knowledge base similar existing Linnaean taxonomy. placing backbone taxonomically highly structured pattern emerges which can integrated via their

Language: Английский

From DNA barcodes to ecology: Meta‐analysis of central European beetles reveal link with species ecology but also to data pattern and gaps

Ecology and Evolution, Journal Year: 2022, Volume and Issue: 12(12)

Published: Dec. 1, 2022

DNA barcoding has been used worldwide to identify biological specimens and delimit species. It represents a cost-effective, fast, efficient way assess biodiversity with help of the public Barcode Life Database (BOLD) accounting for more than 236,000 animal species 10 million barcode sequences. Here, we performed meta-analysis available data central European Coleoptera detect intraspecific genetic patterns among ecological groups in relation geographic distance aim investigate possible link between infraspecific variation ecology. We collected information regarding feeding style, body size, as well habitat biotope preferences. Mantel tests two variants Procrustes analysis, both involving Principal Coordinates Neighborhood Matrices (PCNM) approach, were applied on matrices. However, significance levels too low further use outcome trait investigation: these mean all guilds only 7.5, 9.4, or 15.6% PCNM + PCA, NMDS test, respectively, at best 28% single guild. Our study confirmed that certain traits associated higher diversity foster stronger differentiation. Results suggest increased numbers species, sampling localities, chosen area interest may give new insights explore ecology scope conservation larger scale. beetles distance, investigation.

Language: Английский