A Donor Registry: Genomic Analyses of Posidonia australis Seagrass Meadows Identifies Adaptive Genotypes for Future‐Proofing DOI Creative Commons
Matt J. Nimbs, Tim M. Glasby, Elizabeth A. Sinclair

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(12)

Published: Dec. 1, 2024

ABSTRACT Globally, anthropogenic climate change has caused declines of seagrass ecosystems necessitating proactive restoration approaches that would ideally anticipate future scenarios, such as marine warming. In eastern Australia, estuaries with meadows the endangered Posidonia australi s have warmed and acidified over past decade, communities declined in some estuaries. Securing these valuable habitats will require conservation efforts could be augmented focussed on boosting resilience to climate. Understanding patterns selection where are adapted particular environmental conditions is key for identifying optimal donor material restoration. We used single nucleotide polymorphisms genotype by environment analyses identify candidate loci under putative stressors assess genomic variation allelic turnover along stressor gradients. The most important physicochemical variables driving were associated temperature, water turbidity, pH. developed a preliminary ‘donor registry’ pre‐adapted P. australis genotypes mapping distribution alleles visualise composition each sampled meadow. registry first step select source future‐proofing projects. A next establish manipulative experiments required test whether confer increased resistance multiple stressors.

Language: Английский

Strong genetic differentiation and low genetic diversity in a habitat‐forming fucoid seaweed (Cystophora racemosa) across 850 km of its range DOI Creative Commons
Jane M. Edgeloe, Samuel Starko, Albert Pessarrodona

et al.

Journal of Phycology, Journal Year: 2025, Volume and Issue: unknown

Published: May 3, 2025

Abstract Temperate seaweed forests are among the most productive and widespread habitats in coastal waters. However, they under threat from climate change other anthropogenic stressors. To effectively conserve manage these ecosystems rising pressures, an understanding of genetic diversity structure habitat‐forming seaweeds will be necessary. Australia's Great Southern Reef, a global hotspot endemic diversity, is home to one world's speciose genera, Cystophora (order Fucales). Despite severe declines some species, genomic data on this genus remain limited. We used reduced representation approach (DaRTSeq) investigate racemosa , dominant canopy‐forming across ~850 km its range. Our sequencing captured 4741 high‐quality single nucleotide polymorphisms (SNPs), we distinguished neutral loci those natural selection (i.e., outlier loci). identified strong population high differentiation for both (mean F ST = 0.404) 0.901). Across populations, was low (neutral: mean H E 0.046; outlier: 0.042), with inferred inbreeding (neutral IS 0.531) no evidence isolation‐by‐distance. Several SNPs ( n 70) were observed putatively adaptive, (97%) correlated annual maximum sea surface temperature (SST, °C), indicating local adaptation key ocean variable. results show that C. populations have differentiation, which may increase vulnerability important foundation species change.

Language: Английский

Citations

0
Historic and contemporary biogeographic perspectives on range‐wide spatial genetic structure in a widespread seagrass DOI Creative Commons
Elizabeth A. Sinclair, Renae Hovey, Siegfried L. Krauss

et al.

Ecology and Evolution, Journal Year: 2023, Volume and Issue: 13(3)

Published: March 1, 2023

Abstract Historical and contemporary processes drive spatial patterns of genetic diversity. These include climate‐driven range shifts gene flow mediated by biogeographical influences on dispersal. Assessments that integrate these drivers are uncommon, but critical for testing biogeographic hypotheses. Here, we characterize intraspecific diversity structure across the entire distribution a temperate seagrass to test marine concepts southern Australia. Predictive modeling was used contrast current Posidonia australis its historical during Last Glacial Maximum (LGM). Spatial estimated 44 sampled meadows from geographical species using nine microsatellite loci. distributions were similar, with exception Bass Strait. Genetic clustering consistent three currently recognized provinces largely finer‐scale IMCRA bioregions. Discrepancies found within Flindersian province southwest bioregion, while two regions admixture coincided transitional Clonal highly variable positively associated latitude. differentiation among significantly oceanographic distance. Our approach suggests how shared seascape have influenced capacity P. effectively track sea level changes natural climate cycles over millennia, in particular, recolonization Continental Shelf following LGM. bioregions reflects presence stable barriers, such as oceanic upwellings. This study highlights importance biogeography infer role shaping extant structure.

Language: Английский

Citations

6
Extensive polyploid clonality was a successful strategy for seagrass to expand into a newly submerged environment DOI
Elizabeth A. Sinclair, Jane M. Edgeloe

Published: April 26, 2023

Polyploidy has the potential to allow organisms outcompete their diploid progenitor(s) and occupy new environments. Shark Bay, Western Australia, is a World Heritage Area dominated by temperate seagrass meadows including Poseidon's ribbon weed, Posidonia australis. This at northern extent of its natural geographic range experiences extremes in temperature salinity. Our genomic cytogenetic assessments 10 identified geographically restricted, clones (2n = 20) single location, widespread, high-heterozygosity, polyploid clone 40) all other locations. The spanned least 180 km, making it largest known example any environment on earth. Whole-genome duplication through polyploidy, combined with clonality, may have provided mechanism for P. australis expand into habitats adapt environments that became increasingly stressful progenitor(s). probably formed shallow waters after inundation Bay less than 8500 years ago subsequently expanded via vegetative growth newly submerged habitats.

Language: Английский

Citations

6
Comparative gene co-expression networks show enrichment of brassinosteroid and vitamin B processes in a seagrass under simulated ocean warming and extreme climatic events DOI Creative Commons
Mitchell Booth, Elizabeth A. Sinclair, E. Maria U. Jung

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 26, 2024

Introduction Ocean warming combined with extreme climatic events, such as marine heatwaves and flash flooding threaten seagrasses globally. How cope these challenges is uncertain, particularly for range-edge populations of species Posidonia australis in Shark Bay, Western Australia. Analyzing gene expression while manipulating multiple stressors provides insight into the genetic response resilience to climate change. We conducted a study on polyploid clone P. during an 18-week mesocosm experiment assess responses single future change-associated stressors. Methods Plants were exposed (1) ocean temperature (baseline +1.5°C) followed by simulated heat wave +5.5°C), (2) light deprivation simulating observed heatwave driven turbidity (95% shade) at baseline temperatures, or (3) both simultaneously. Basal leaf meristems sampled analysis using RNA-seq four time points experiment. Weighted co-expression network analysis, GO term enrichment, KEGG pathway enrichment analyses used identify stress responses. Results Shaded plants showed specific shade avoidance (programmed cell death) after three weeks stress, before any heated tanks response. positively correlated programmed death stress-related processes end Once temperatures (+1.5°C) effect, (e.g., ROS scavenging polyamine metabolism) was present. Vitamin B processes, RNA polymerase II processes. light-related meristematic phase changes expressed addition MHW. Heated growth signatures well trehalose salicylic acid metabolism. Brassinosteroid-related significantly enriched all stressor treatments points, except isolated heat-stressed initiation. Discussion Gene interaction between waves turbidity-induced reduction support geographical scale mortality suggesting that even this giant will be negatively impacted more change projections.

Language: Английский

Citations

1
Early seedling development and survival of seagrasses Posidonia australis and P. sinuosa using different seed‐based restoration methods DOI Creative Commons
Rizqi Aimmatul Maulidiyah, Marion L. Cambridge, Rachel Austin

et al.

Restoration Ecology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

Seagrass seed‐based restoration has been developed in several places worldwide, but disturbance at the vulnerable initial stages of seedling recruitment proved to be a major bottleneck successful restoration. A detailed investigation seagrass survival and growth earliest development is an important contribution developing techniques improve establishment survival. Here, we investigated early Posidonia australis P. sinuosa as well quantifying variation under different methods. Early was documented indoor aquaria during first month after fruit dehiscence. In second ex situ experiment, determined effects three methods (surface sowing, planting, planting inside hessian bag) on over 2 months life. primary root with hairs 7 days. After 1 month, roots were 20–60 mm length, firmly establishing into sediment. Compared surface bag treatments did not significantly affect for increased mortality , although greater (shoot lengths approximately 50 40% longer, respectively). These aquarium‐scale experiments suggest that would enhance larger scales some species, promoting damaged habitats.

Language: Английский

Citations

1
A Donor Registry: Genomic Analyses of Posidonia australis Seagrass Meadows Identifies Adaptive Genotypes for Future‐Proofing DOI Creative Commons
Matt J. Nimbs, Tim M. Glasby, Elizabeth A. Sinclair

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(12)

Published: Dec. 1, 2024

ABSTRACT Globally, anthropogenic climate change has caused declines of seagrass ecosystems necessitating proactive restoration approaches that would ideally anticipate future scenarios, such as marine warming. In eastern Australia, estuaries with meadows the endangered Posidonia australi s have warmed and acidified over past decade, communities declined in some estuaries. Securing these valuable habitats will require conservation efforts could be augmented focussed on boosting resilience to climate. Understanding patterns selection where are adapted particular environmental conditions is key for identifying optimal donor material restoration. We used single nucleotide polymorphisms genotype by environment analyses identify candidate loci under putative stressors assess genomic variation allelic turnover along stressor gradients. The most important physicochemical variables driving were associated temperature, water turbidity, pH. developed a preliminary ‘donor registry’ pre‐adapted P. australis genotypes mapping distribution alleles visualise composition each sampled meadow. registry first step select source future‐proofing projects. A next establish manipulative experiments required test whether confer increased resistance multiple stressors.

Language: Английский

Citations

0