Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(1)
Published: Jan. 9, 2023
Atmospheric nitrogen (N) deposition is a worldwide environmental problem and threatens biodiversity ecosystem functioning. Understanding the responses of community dynamics assembly processes abundant rare soil bacterial taxa to anthropogenic N enrichment vital for management N-polluted forest soils.
Language: Английский
The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 755, P. 142449 - 142449
Published: Sept. 22, 2020
Language: Английский
Citations
146
Global Change Biology, Journal Year: 2021, Volume and Issue: 27(16), P. 3939 - 3950
Published: May 16, 2021
Abstract Anthropogenic nitrogen (N) input is known to alter plant and microbial α‐diversity, but how N enrichment influences β‐diversity of communities remains poorly understood. Using a long‐term multilevel addition experiment in temperate steppe, we show that plant, soil bacterial fungal exhibited different responses their input. Plant decreased linearly as increased, result increased directional environmental filtering, where properties largely explained variation β‐diversity. Soil first then with increasing input, which was best by corresponding changes heterogeneity. β‐diversity, however, remained unchanged across the gradient, properties, heterogeneity together explaining an insignificant fraction reflecting importance stochastic community assembly. Our study demonstrates divergent effect on assembly communities, emphasizing need examine closely associated fundamental components (i.e., plants microorganisms) ecosystems gain more complete understanding ecological consequences anthropogenic enrichment.
Language: Английский
Citations
129
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: April 3, 2024
Abstract Nutrient enrichment is a major global change component that often disrupts the relationship between aboveground biodiversity and ecosystem functions by promoting species dominance, altering trophic interactions, reducing stability. Emerging evidence indicates nutrient also reduces soil weakens belowground functions, but underlying mechanisms remain largely unclear. Here, we explore effects of on properties, biodiversity, multiple through 13-year field experiment. We show acidification induced enrichment, rather than changes in mineral carbon (C) availability, primary factor negatively affecting diversity multifunctionality. Nitrogen phosphorus additions significantly reduce pH, bacteria, fungi nematodes, as well an array related to C cycling. Effects microbial have negative consequences at higher levels microbivorous nematodes. These results indicate nutrient-induced can cascade up its impacts along food webs influence functioning, providing novel insight into which influences community properties.
Language: Английский
Citations
65
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 926, P. 172100 - 172100
Published: March 29, 2024
Language: Английский
Citations
25
iMeta, Journal Year: 2024, Volume and Issue: 3(3)
Published: April 15, 2024
We investigated soil bacterial and fungal communities, constructed co-occurrence networks, estimated traits along a gradient of nitrogen (N) input. The results showed that networks complexity decreased with increasing N ratio negative to positive cohesion input, suggesting the declined competitive but strengthened cooperative interactions. However, network did not change under enrichment. In addition, input stimulated copiotroph/oligotroph ratio, ribosomal RNA operon (rrn) copy number, guanine-cytosine (GC) content bacteria, shifting life history strategy toward copiotroph increased r-/K-strategy ratio. Piecewise structural equation modeling further revealed reduction in was directly regulated by rather than reduced richness. Our study reveals mechanisms through which microbial regulate interactions shape global changes. Nitrogen deposition induced emission nitrogenous compounds from combustion fossil fuels application fertilizers has risen 200% is projected double 2050 various regions worldwide [1]. subsequent enrichment poses serious threats plant diversity, community, functioning terrestrial ecosystems, particularly grasslands [2]. Ecosystem function associated only changes plants also dynamics microbes response [3]. Alterations populations can trigger among microorganisms, play an important role maintaining complexity, stability, [4]. Thus, understanding interaction essential prediction ecosystem processes grassland ecosystems. Microbial comprises components generally links few recent studies on have produced mixed results, irrespective richness [5-7]. Specifically, some reported increase following application, attributing number keystone taxa [6]. Conversely, investigation 150-year manipulative significant coupling decline relationships within communities [5]. Furthermore, one multilevel indicated higher lower diminished same trend [7]. Across those studies, responses were found align alterations complexity. Different regulatory effect cooperation community ecologists proposed existence facilitates high-order consequently enhances stability That said, enhancement contributes Accordingly, above observations simplified seem contradictory this theoretical coexistence conceptual statement between (negative) Therefore, despite extensive investigations into diversity ecosystems globally, there still severe lack information regarding how respond their underlying mechanisms. Here, we investigate temperate China. This aims explore elucidate potential driving factors will address questions: (1) Whether lead diminution enrichment? (2) How do (competitive) (3) Given fungi are more closely connected plants, exert pronounced impact complexity? experimental site located semiarid Duolun County, Inner Mongolia, A Latin square design utilized since 2003, comprising total 8 levels (0, 1, 2, 4, 8, 16, 32, 64 g m−2 y−1). Soil samples collected August 2016 used for sequencing chemical analyses (see Supporting Information: Methods). With diminishing nodes edges, average degree, clustering coefficient, first axis principal component analysis (PC1) characteristics (Figure 1A,B S1). Similar topological parameters, total, positive, cohesion, negative/positive value 1C). alter S2). content, PC1 representing overall gradually elevated 2A). Total exhibited correlation 2B). (SEM) dissolved inorganic (DIN) decreasing pH, altered negatively affected had direct association no 2C). line greater rrn high conditions [8], DIN copiotrophic S3). GC application. According rule molecular biosynthesis, each at base pairs requires atoms while adenine-thymine (AT) 7 [9]. "resource-driven selection" theory proposes limitation strong selective force causes relatively low [10, 11]. Consequently, would result nucleotide sequences G+C bias alleviating limitation. previous reports positively correlated pH regional scale [11], related our S4). may be due decrease caused extra Overall, indicates tends r-selection strategy. supports grassland. accompanied work [12] Note ascribed loss oligotrophic S5), resulted shift When controlling partial longer SEM implying tightness connections driven strategies 2B,C S6). Oligotrophic acquire nutrients or energy complex recalcitrant matter [13]. acquisition process consists multiple metabolic pathways involving establishing effective stronger [8]. stimulates growth consequent fresh carbon inputs S7), producing sufficient supply labile substrates [14]. excessive as well substrate promotes independence, weakening leading less connectivity [5], thus simplifying networks. finding mediates points out genetic coding co-occurrence. speculated linked deconstruction community. Interaction chains refer indirect impacts species another abundance third beyond pairwise [15]. These emerged produce obtaining diverse robust network, turn, suggest low-order represents strength. competition Opposite typical [7], diminishment reinforcement alleviates could weaken fast metabolism metabolites facilitate exchange materials called cross-feeding enhance [16]. Correspondingly, enhanced S8). Moreover, certain promote equalizing fitness other [17]. such competitors others concurrent emphasize coexistence. unchanged support hypothesis responsive attributed physiological morphological properties fungi. great capacity mineralize organic [18]. they use efficiency biomass accumulation C/N [19]. Those two dependence manifesting insensitive thick cell walls intrinsic spores exhibit tolerance acidification metal toxicity [18], remaining stable feature mycelium enables it penetrate spread underground establish hyphal [20]. extend over wider spatial distribution therefore resistant environmental change. conclusion, demonstrates distinct patterns steppe. As increased, downward trend, remained unaltered. It worth noting derived decade cumulative time, temporal should considered when extrapolating. Nevertheless, best knowledge, empirical report predominantly provides insights comprehensive modulate Weixing Liu conceived idea supervised study. Chao Wang analyzed data. wrote manuscript all authors. Ziyue Shi, Aogui Li, Tianyi Geng, Lingli made contributions revision manuscript. All authors read final approved publication. supported National Natural Science Foundation China (42141006, 32171595, 31770530). declare conflict interest. No animals humans involved (The 16S ITS submitted NCBI accession numbers PRJNA573484 http://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA573484) PRJNA573488 (http://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA573488). data, scripts, Table S saved GitHub (http://github.com/ChaoWang-01/code-for-imeta). Supplementary (methods, figures, graphical abstract, slides, videos, Chinese translated version, update materials) online DOI iMeta http://www.imeta.science/. Figure S1: edges different treatments. S2: Fungal topology parameters S3: Effects traits. S4: S5: operational taxonomic unit (OTU) trophic types S6: S7: Aboveground S8: Please note: publisher responsible functionality any supporting supplied Any queries (other missing content) directed corresponding author article.
Language: Английский
Citations
23
Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(2)
Published: Jan. 9, 2025
Pesticide application is essential for stabilizing agricultural production. However, the effects of increasing pesticide diversity on soil microbial functions remain unclear, particularly under varying nitrogen (N) fertilizer management practices. In this study, we investigated stochasticity microbes and multitrophic networks through amplicon sequencing, assessed community related to carbon (C), N, phosphorus (P), sulfur (S) cycling, characterized dominant bacterial life history strategies via metagenomics along a gradient two N addition levels. Our findings show that higher enriches abundance specialists opportunists capable degrading or resisting pesticides, reducing proportion generalists in absence addition. These shifts can complicate networks. Under increased diversity, selective pressure may drive bacteria streamline their average genome size conserve energy while enhancing C, P, S metabolic capacities, thus accelerating nutrient loss. comparison, was found reduce niche differentiation at mitigating impacts network complexity functional traits associated with ultimately alleviating results reveal contrasting different input scenarios emphasize strategic mitigate ecological use systems.
Language: Английский
Citations
3
The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 795, P. 148848 - 148848
Published: July 3, 2021
Language: Английский
Citations
66
Microbial Ecology, Journal Year: 2022, Volume and Issue: 85(1), P. 221 - 231
Published: Jan. 18, 2022
Language: Английский
Citations
48
New Phytologist, Journal Year: 2022, Volume and Issue: 237(1), P. 279 - 294
Published: Sept. 30, 2022
Summary Nitrogen (N) enrichment poses threats to biodiversity and ecosystem stability, while arbuscular mycorrhizal (AM) fungi play important roles in stability functioning. However, the ecological impacts, especially thresholds of N potentially causing AM fungal community shifts have not been adequately characterized. Based on a long‐term field experiment with nine addition levels ranging from 0 50 g m −2 yr −1 temperate grassland, we characterized response patterns enrichment. Arbuscular biomass continuously decreased increasing levels. diversity did significantly change below 20 , but dramatically at higher levels, which drove unstable state. Structural equation modeling showed that decline could be well explained by soil acidification, whereas key driving factors for shifted nitrogen : phosphorus (N P) ratio pH Different aspects communities (biomass, composition) respond differently Thresholds substantial this grassland are identified.
Language: Английский
Citations
48
Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 12
Published: Jan. 20, 2022
Although a large number of studies have reported the importance microbial communities in terrestrial ecosystems and their response to nitrogen (N) application, it is not clear arid alpine wetlands, mechanisms involved need be clarified. Therefore, soil community swamp meadow short-term (1 year) N application (CK: 0, N1: 8, N2: 16 kg⋅N⋅hm-2⋅a-1) was studied using 16S/ITS rRNA gene high-throughput sequencing technology. Results showed that had no significant effect on diversity, but significantly changed bacterial structure. N1 N2 treatments reduced relative abundance Chloroflexi (18.11 32.99% lower than CK, respectively). treatment Nitrospirae (24.94% CK). Meanwhile, potential function partial sulfur (S) cycling community. For example, compared with nitrate respiration decreased by 35.78-69.06%, dark sulfide oxidation 76.36-94.29%. little fungal structure function. In general, directly affected indirectly through available potassium, while organic carbon an important factor affecting
Language: Английский
Citations
47