Cited by Investigating fine-scale breeding habitat use by amphibians in a continuous wetland using environmental DNA

Targeting Terrestrial Vertebrates With eDNA: Trends, Perspectives, and Considerations for Sampling DOI

Joshua P. Newton, Morten E. Allentoft, Philip W. Bateman

et al.

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 1, 2025

ABSTRACT Terrestrial vertebrates are experiencing worldwide population declines and species extinctions. To effectively conserve remaining populations species, rapid, cost‐effective, scalable methods needed to complement longstanding monitoring methods. Increasingly, environmental DNA (eDNA)‐based approaches being used for terrestrial vertebrate biomonitoring within a range of environments. However, as we move eDNA onto land, presented with new set challenges. This necessitates the development “best‐practice” sample collection guidelines systems purpose detecting vertebrates. address these needs, conducted systematic literature review 143 peer‐reviewed papers applying (excluding Lissamphibia) that were published between 2012 2023. We summarize use biomonitoring, focusing on study design field techniques. Over decade observe steady growth in annual number publications, 3 33 The majority reviewed studies targeted mammals temperate forest regions. While an equal focused metabarcoding approach assess community taxon composition and/or species‐specific detection methods, novel uses increasingly published. These include animal behavior genetics. record three types sampling strategies, eight different substrate types, seven preservation suggesting there is no “one size fits all” eDNA‐based methodology when With multitude aims, across environments, target organisms ecologies, standardization extremely challenging. table known factors influencing Furthermore, identify five key considerations be addressed targeting aim guiding decision making.

Language: Английский

Citations

A review of applications of environmental DNA for reptile conservation and management DOI

Bethany Nordstrom, Nicola J. Mitchell, Margaret Byrne

et al.

Ecology and Evolution, Journal Year: 2022, Volume and Issue: 12(6)

Published: June 1, 2022

Abstract Reptile populations are in decline globally, with total reptile abundance halving the past half century, and approximately a fifth of species currently threatened extinction. Research on distributions, population trends, trophic interactions can greatly improve accuracy conservation listings planning for recovery, but data deficiency is an impediment many species. Environmental DNA (eDNA) detect measure community diversity at diverse spatio‐temporal scales, especially useful detection elusive, cryptic, or rare species, making it potentially very valuable herpetology. We aim to summarize utility eDNA as tool informing management discuss benefits limitations this approach. A literature review was conducted collect all studies that used focus ecology, conservation, management. Results search summarized into key discussion points, also draws from other taxa highlight methodological challenges identify future research directions. has had limited application reptiles, relative vertebrate groups, little use regions high richness. techniques have been more successfully applied aquatic reptiles than terrestrial most (64%) focused habitats. Two four reptilian orders dominate existing (56% Testudines, 49% Squamata, 5% Crocodilia, 0% Rhynchocephalia). Our provides direction emerging ecology when be paired traditional monitoring approaches. Technologies associated rapidly advancing, become sensitive accessible, we expect will increasingly addressing knowledge gaps reptiles.

Language: Английский

Citations

Detection of vertebrates from natural and artificial inland water bodies in a semi‐arid habitat using eDNA from filtered, swept, and sediment samples DOI

Rupert McDonald,

Philip W. Bateman, Christine E. Cooper

et al.

Ecology and Evolution, Journal Year: 2023, Volume and Issue: 13(4)

Published: April 1, 2023

Biomonitoring is vital for establishing baseline data that needed to identify and quantify ecological change inform management conservation activities. However, biomonitoring biodiversity assessment in arid environments, which are predicted cover 56% of the Earth's land surface by 2100, can be prohibitively time consuming, expensive, logistically challenging due their often remote inhospitable nature. Sampling environmental DNA (eDNA) coupled with high-throughput sequencing an emerging method. Here we explore application eDNA metabarcoding various sampling approaches estimate vertebrate richness assemblage at human-constructed natural water sources a semi-arid region Western Australia. Three methods: sediment samples, filtering through membrane pump, sweeping body, were compared using two assays, 12S-V5 16smam, 120 samples collected from four gnammas (

Language: Английский

Citations

Measuring the Impact of Conservation: The Growing Importance of Monitoring Fauna, Flora and Funga DOI

P. J. Stephenson, María Cecilia Londoño, Paulo A. V. Borges

et al.

Diversity, Journal Year: 2022, Volume and Issue: 14(10), P. 824 - 824

Published: Sept. 30, 2022

Many stakeholders, from governments to civil society businesses, lack the data they need make informed decisions on biodiversity, jeopardising efforts conserve, restore and sustainably manage nature. Here we review importance of enhancing biodiversity monitoring, assess challenges involved identify potential solutions. Capacity for monitoring needs be enhanced urgently, especially in poorer, high-biodiversity countries where gaps are disproportionately high. Modern tools technologies, including remote sensing, bioacoustics environmental DNA, should used at larger scales fill taxonomic geographic gaps, tropics, marine freshwater biomes, plants, fungi invertebrates. Stakeholders follow best practices, adopting appropriate indicators using counterfactual approaches measure attribute outcomes impacts. Data made openly freely available. Companies invest collecting required enhance sustainability their operations supply chains. With soon commit post-2020 global framework, time is right a concerted push monitoring. However, action scale needed now if results-based management adequately conserve ecosystem services all depend on.

Language: Английский

Citations

Urban landscape-level biodiversity assessments of aquatic and terrestrial vertebrates by environmental DNA metabarcoding DOI

Shan Zhang, Jindong Zhao, Meng Yao

et al.

Journal of Environmental Management, Journal Year: 2023, Volume and Issue: 340, P. 117971 - 117971

Published: April 27, 2023

Language: Английский

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Continuous daily sampling of airborne eDNA detects all vertebrate species identified by camera traps DOI

Marcel Polling,

Ralph Buij,

Ivo Laros

et al.

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(4)

Published: July 1, 2024

Abstract Ongoing pressures on global biodiversity require conservation action that is not possible without effective biomonitoring. Terrestrial vertebrate surveys are commonly performed using camera traps, a time‐intensive method known to miss many small or arboreal species and birds. Recent advances have shown airborne eDNA be potentially suitable technique more effectively monitor communities in time‐ cost‐effective manner. Here, we test whether commercially available air samplers collect particles 24/7 during 1‐week period can used detect the presence of vertebrates through eDNA. The results compared trap records at three locations with differing habitats Netherlands. Simultaneous sampling different for 3 weeks resulted detection 154 taxa, which majority were birds mammals (113 33 species, respectively), along four fish amphibian species. All observed traps also retrieved via eDNA, although every day sampling. Burkard spore trap, routinely pollen monitoring, showed highest number only samples when mammal was detected it remained undetected We unique indicative habitat they living. However, could account for. multitude found indicate sensitivity method; however, subsequent studies should prioritize validation these findings alternative biomonitoring approaches.

Language: Английский

Citations

Monitoring terrestrial rewilding with environmental DNA metabarcoding: a systematic review of current trends and recommendations DOI

Clare Cowgill,

James Gilbert, Ian Convery

et al.

Frontiers in Conservation Science, Journal Year: 2025, Volume and Issue: 5

Published: Jan. 2, 2025

Introduction Rewilding, the facilitation of self-sustaining and resilient ecosystems by restoring natural processes, is an increasingly popular conservation approach potential solution to biodiversity climate crises. Outcomes rewilding can be unpredictable, monitoring essential determine whether are recovering. Metabarcoding, particularly environmental DNA (eDNA), revolutionizing could play important role in understanding impacts but has mostly been applied within aquatic systems. Methods This systematic review focuses on applications eDNA metabarcoding terrestrial monitoring, with additional insights from bulk ingested DNA. We examine publication trends, choice sampling substrate focal taxa, investigate how well performs compared other methods (e.g. camera trapping). Results Terrestrial represented a small proportion total papers, forests most studied system, soil water substrates, vertebrates targeted taxa. Most studies focused measuring species richness, few included analyzes functional diversity. Greater richness was found when using multiple took this approach. Metabarcoding did not consistently outperform terms number vertebrate taxa detected, likely influenced marker, habitat. Discussion Our findings indicate that metabarcoding, eDNA, key rewilding, further ground- truthing needed establish appropriate experimental pipelines for target system interest. Systematic Review Registration https://osf.io/38w9q/?view_only=47fdab224a7a43d298eccbe578f1fcf0 , identifier 38w9q.

Language: Английский

Citations

Mosquito‐derived ingested DNA as a tool for monitoring terrestrial vertebrates within a peri‐urban environment DOI

Christine Chivas, Adam Stow, Andrew J. Harford

et al.

Ecosphere, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 1, 2025

Abstract Global biodiversity is declining at an alarming rate. Consequently, there a pivotal need to determine the occurrences and distributions of threatened species. Monitoring detection approaches are traditionally reliant on capture (traps cameras), as well observations. However, these time‐consuming skewed toward large and/or common Invertebrate ingested DNA (iDNA) being increasingly used novel approach for indirectly monitoring terrestrial vertebrates via their in invertebrates with hematophagous, coprophagous, or saprophagous feeding strategies. This study aimed examine vertebrate diversity which could be retrieved using mosquito‐derived iDNA peri‐urban setting. Furthermore, also examined influence human blocking primer application multiple primers targeted taxa. Sampling was performed Sydney, Australia, environment adjacent both urbanized protected environments. As means ensuring that sampling by nonscientists, domestically available light traps were used. In total, 118 mosquitoes captured. extracted from individual amplified four different primers, targeting vertebrates, mammals, birds, without (except bird polymerase chain reactions). The overall reveals broad species 10 avian taxa six mammalian taxa, including native non‐native varying body sizes behavioral characteristics. Both multi‐locus use revealed additional diversity. offers potential important tool local land managers citizen science projects vertebrates.

Language: Английский

Citations

Investigating Fine‐Scale Breeding Habitat Use by Amphibians in a Continuous Wetland Using Environmental DNA DOI

Julie Morgane Guenat,

Antoine Gander,

Luca Fumagalli

et al.

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 1, 2025

ABSTRACT Designing effective conservation plans to protect species from extinction requires a comprehensive understanding of their ecology. Conventional methods used investigate habitat use are time‐consuming, and the detectability cryptic is often insufficient. Environmental DNA (eDNA)‐based approaches provide complementary tool traditional monitoring for ecosystem assessment. Nevertheless, our knowledge, such have rarely been applied at fine scale in continuous wetland environment. Here, we an eDNA metabarcoding approach characterize breeding local amphibian wet meadow expanse along southern shore Lake Neuchâtel, Switzerland. We retrieved six out seven expected be present. tested influence abiotic environmental variables on overall assemblages individual occurrences. showed that main factor structuring was water temperature distribution three associated with several variables. Our results indicate detection promising tools study species' ecology small habitats.

Language: Английский

Citations

Multi-Primer-Based Environmental DNA Survey for Vertebrate Biodiversity Monitoring in Protected Island Wetlands DOI

Gawoo Kim,

Yujin Kang, Youngkeun Song

et al.

Global Ecology and Conservation, Journal Year: 2025, Volume and Issue: unknown, P. e03474 - e03474

Published: Feb. 1, 2025

Language: Английский

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