Environmental DNA, Journal Year: 2023, Volume and Issue: 5(4), P. 818 - 820
Published: May 3, 2023
Language: Английский
Environmental DNA, Journal Year: 2024, Volume and Issue: 6(4)
Published: July 1, 2024
Abstract Ongoing pressures on global biodiversity require conservation action that is not possible without effective biomonitoring. Terrestrial vertebrate surveys are commonly performed using camera traps, a time‐intensive method known to miss many small or arboreal species and birds. Recent advances have shown airborne eDNA be potentially suitable technique more effectively monitor communities in time‐ cost‐effective manner. Here, we test whether commercially available air samplers collect particles 24/7 during 1‐week period can used detect the presence of vertebrates through eDNA. The results compared trap records at three locations with differing habitats Netherlands. Simultaneous sampling different for 3 weeks resulted detection 154 taxa, which majority were birds mammals (113 33 species, respectively), along four fish amphibian species. All observed traps also retrieved via eDNA, although every day sampling. Burkard spore trap, routinely pollen monitoring, showed highest number only samples when mammal was detected it remained undetected We unique indicative habitat they living. However, could account for. multitude found indicate sensitivity method; however, subsequent studies should prioritize validation these findings alternative biomonitoring approaches.
Language: Английский
Citations
4
Environmental DNA, Journal Year: 2022, Volume and Issue: 5(1), P. 117 - 131
Published: Dec. 6, 2022
Abstract Arthropods are essential in maintaining healthy and productive agricultural ecosystems. Agricultural crops such as apples typically pollinated by domesticated honey bees, but wild bees other arthropod flower visitors also contribute to pollination. Flower can be natural enemies of crop‐pests or herbivores. Biodiversity is under pressure knowledge wildflower an important tool designing orchards that support high functional biodiversity. In our study, we assessed the diversity four Danish apple using both molecular nonmolecular techniques study communities Arthropod DNA collected from flowers was analyzed a metabarcoding approach mitochondrial COI marker, while pollinators were recorded through visual assessment surveys. These complementary resulted total 19 taxa detected. Nonbee arthropods constituted large proportion detected methods (84%, 16 taxa). Metabarcoding 12 had 83% species resolution. Visual census recovered visiting groups order level (Coleoptera, Diptera, Hymenoptera Lepidoptera) not provided relative abundance data, which possible with methods. We demonstrated utilizing assess communities, able obtain broader overview fauna present. The methodology used outcome this inform tailor suitable arthropod‐pest management practices increase crop yield maintain systems.
Language: Английский
Citations
16
PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e14772 - e14772
Published: April 26, 2023
Understanding roosting behaviour is essential to bat conservation and biomonitoring, often providing the most accurate methods of assessing population size health. However, roosts can be challenging survey, e.g ., physically impossible access or presenting risks for researchers. Disturbance during monitoring also disrupt natural present material such as disrupting hibernation cycles. One solution this use non-invasive approaches. Environmental (e)DNA has proven especially effective at detecting rare elusive species particularly in hard-to-reach locations. It recently been demonstrated that eDNA from vertebrates carried air. When collected semi-confined spaces, airborne provide remarkably profiles biodiversity, even complex tropical communities. In study, we deploy novel collection first time a setting approach survey difficult potential neotropics. Using eDNA, confirmed presence bats nine out 12 roosts. The identified matched previous records roost obtained photographic live capture methods, thus demonstrating utility approach. We detected white-winged vampire ( Diaemus youngi ) which had never area but was long suspected based on range maps. addition bats, several non-bat vertebrates, including big-eared climbing rat Ototylomys phyllotis ), previously observed around our study area. other local known vicinity. detect new monitor populations, when turnover rapid, could maximize efficiency surveyors while minimizing disturbance animals. This presents applied ecological analysis moving beyond proof concept demonstrate clear technology wild.
Language: Английский
Citations
8
Advances in ecological research/Advances in Ecological Research, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 34
Published: Jan. 1, 2023
Language: Английский
Citations
7
Journal of Animal Ecology, Journal Year: 2024, Volume and Issue: 93(8), P. 1003 - 1021
Published: June 12, 2024
Abstract Terrestrial invertebrates are highly important for the decomposition of dung from large mammals. Mammal has been present in many Earth's ecosystems millions years, enabling evolution a broad diversity dung‐associated that process various components dung. Today, herbivorous mammals increasingly introduced to with aim restoring ecological functions formerly provided by their extinct counterparts. However, we still know little about ecosystem and nutrient flows these rewilded ecosystems, including dynamics decomposition. In fact, succession insect communities is an area limited research attention also outside rewilding context. this study, use environmental DNA metabarcoding Galloway cattle experimental set‐up investigate invertebrate functional over time span 53 days, starting deposition. We find strong signal successional change community composition, species directly dependent on as resource. While several were detected consistently across sampling period, others appeared confined either early or late stages. believe indicative evolutionary adaptation dynamic resource, showing niche partitioning temporal scale. our results show high within groups Our findings such redundancy suggest stability community, ready fill vacant niches if other disappear. Importantly, might buffer related against change. Interestingly, alpha peaked after approximately 20–25 days both meadow pasture habitats, did not decrease substantially during probably due preservation eDNA disappearance visiting invertebrates, detection tissue remains cryptic life
Language: Английский
Citations
2
PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e15171 - e15171
Published: April 14, 2023
Air is a medium for dispersal of environmental DNA (eDNA) carried in bioaerosols, yet the atmosphere mostly unexplored as source genetic material encompassing all domains life. In this study, we designed and deployed robust, sterilizable hardware system airborne nucleic acid capture featuring active filtration quantifiable, controllable volume air high-integrity chamber to protect sample from loss or contamination. We used our on an aircraft across multiple height transects over major aerosolization sources collect eDNA, coupled with high-throughput amplicon sequencing using metabarcoding markers targeting bacteria, plants, vertebrates test hypothesis large-scale presence these bioaerosols throughout planetary boundary layer lower troposphere. Here, demonstrate that multi-taxa assemblages inventoried up 2,500 m airplane-mounted are reflective survey area show previously unreported species detections ( i.e ., Allium sativum L). also pioneer aerial flight grid standardized atmospheric sampling aeroallergens light limited resources. Our results eDNA terrestrial detectable high altitude sampler usefulness monitoring campaigns. However, work underscores need improved marker choices reference databases column, particularly eukaryotes. Taken together, findings reveal strong connectivity mixing terrestrial-associated ground level atmosphere, recommend parameters indices considering lifting action, instability, potential convection be incorporated future surveys eDNA. Overall, establishes foundation campaigns comprehensively economically inventory bioaerosol emissions impacts at scale, enabling transformative opportunities technology.
Language: Английский
Citations
6
Planta, Journal Year: 2023, Volume and Issue: 258(6)
Published: Nov. 13, 2023
Language: Английский
Citations
6
Molecular Ecology Resources, Journal Year: 2024, Volume and Issue: 24(7)
Published: Aug. 8, 2024
In the context of looming global biodiversity loss, effective species detection represents a critical concern for ecological research and management. Environmental DNA (eDNA) analysis, which refers to collection taxonomic identification genetic fragments that are shed from an organism into its surroundings, emerged approximately 15 years ago as sensitive tool detection. Today, one frontiers eDNA concerns analysis material in dust other airborne materials, termed analysis. As study matures, it is appropriate time review foundational emerging studies make up current literature, use reviewed literature summarize, synthesize, forecast major challenges opportunities this advancing front. Specifically, we "ecology eDNA" framework organize our findings across origin, state, transport, fate materials environment, summarize what so far known their interactions with surrounding abiotic biotic factors, including population community ecologies ecosystem processes. Within work identify key challenges, opportunities, future directions associated application development. Lastly, discuss development applications, partnerships, messaging promote growth field. Together, broad potential rate at accelerating field suggest sky's limit science.
Language: Английский
Citations
1
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Aug. 8, 2024
Assessments of biodiversity and ecosystem status can benefit from DNA metabarcoding as a means to streamline sample processing specimen identification. Moreover, the fixation medium instead precious material introduces straightforward protocols that allow subsequent focus on certain organisms detected among preserved specimens. In this study, we present proof concept via analysis freshwater invertebrate samples Tatra Mountain lakes (Slovakia). Besides highlighting match between lake-specific environmental conditions results our fixative metabarcoding, observed an option fine-tune time: prefer two weeks over day or month. This effect emerged presence/absence individual taxa rather than coarse per-sample records taxonomic richness, demonstrating studies—and efforts optimize their protocols—can use robust metrics explore even subtle trends. We also provide evidence might better capture large species terrestrial meiofauna.
Language: Английский
Citations
1
Journal of Economic Entomology, Journal Year: 2024, Volume and Issue: 117(3), P. 918 - 927
Published: May 14, 2024
As large cities begin to overrun their landfill capacities, they look for alternative locations handle the waste stream. Seeing an opportunity bring in revenue, rural communities offer municipal landfills. However, many are also places of agricultural production, which vulnerable attacks by invasive insect species, could be present green yard waste, component most likely contain agriculturally harmful species. We used environmental DNA (eDNA) determine whether a pathway species enter and establish landfill-receiving community. identified several target that coming from Vancouver, BC, Canada, Central Washington State, USA. sampled 3 sites every 2 weeks June October 2019 2020. was extracted nearly 400 samples subjected amplification with COI barcoding primers followed sequencing identify insects samples. Sequence analyses list: pests deciduous tree fruits generalist root-feeding crop pest. This eDNA technique useful identifying potential may prove important tool informing policy on movement biological material across borders stemming spread
Language: Английский
Citations
0