The Impact of Flow Velocity on Environmental DNA Detectability for the Application in River Systems

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(3)

Published: May 1, 2025

ABSTRACT Organisms perpetually release genetic material in their surroundings, referred to as environmental DNA (eDNA), which can be captured and subsequently analyzed detect biodiversity across the tree of life. In lotic, dynamic environments, little is known about specific factors that affect concentration eDNA between by host its dissemination into environment. This gap knowledge introduces significant uncertainty when applying a monitoring tool. Our objective provide insight on concentrations ecosystems representative rivers streams. To this end, we conducted series laboratory experiments rotating circular (annular) flume, allows for extended degradation under conditions flow. Here, show flow velocity impacts observed over time. results suggest flow‐induced transport keeps suspension, reducing removal from water column, increased eDNA. We temporary increase early phase flume experiment with highest velocity. seems due combination low rates high shear stress, fragment homogenize particles column. The our study importance better understanding assessing detection probability eDNA, both controlled larger‐scale conditions.

Language: Английский

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Eel ramps and environmental DNA reveal a preliminary recruitment window of the American Eel in Texas

Marine and Coastal Fisheries, Journal Year: 2025, Volume and Issue: 17(3)

Published: May 1, 2025

ABSTRACT This study is the first to document glass American Eels Anguilla rostrata in Texas, and it provides critical information about recruitment timing along northern Gulf Coast. The Eel a facultative catadromous fish. Data are lacking related juvenile (glass eel elver) into bays estuaries of Mexico. Objective goal was elver distribution for Texas. Methods implemented year-round monitoring rivers streams using ramps environmental DNA (eDNA) increase likelihood detecting even highly sporadic events. Thirteen sites were identified based on their connectivity coastal estuarine waters ability support gravity-fed ramp. Ramps deployed during June–July 2022 checked weekly 1 year. A subset nine ramp also monitored eDNA each check. Results caught one 13 ramps. In total, 26 eels (19 7 elvers) captured. Captures began January, captures elvers ended May. detected at eight that eDNA. Environmental detection rates highest only site where Conclusions Positive samples temporally associated with window observed through Continued deployment estimated planned 2024 2025, more frequent checks. will be conducted assisting natural resource agencies determining conservation management needs

Language: Английский

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Monitoring the threatened Harttiella (Siluriformes, Loricariidae) with environmental DNA: A comparison between metabarcoding and targeted digital PCR

Aquatic Conservation Marine and Freshwater Ecosystems, Journal Year: 2024, Volume and Issue: 34(2)

Published: Feb. 1, 2024

Abstract Environmental DNA has emerged as an efficient alternative to traditional sampling methods. A standard multispecies or a targeted single‐species approach can be used for analysing environmental samples. The costs, benefits, and drawbacks associated with these two approaches are quite different. Here, comparison between metabarcoding species assay using digital PCR (dPCR) threatened Harttiella occurring in French Guiana ( lucifer H . nsp. “Makwali”) was performed. Samples were collected 11 sites of the upper Maroni River drainage basin located Galbao mountain range, known host species. “Teleo” primer approach. To implement new dPCR assay, specific primers probes developed. This detected seven sites. All also positive detection, habitat characteristics favourable study demonstrated that both (metabarcoding) monitoring H. “Makwali” From conservation point view, we recommend use update spatial distribution species, while method temporal follow‐up populations.

Language: Английский

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Comparative evaluation for the performance of environmental DNA and RNA analyses targeting mitochondrial and nuclear genes from ayu (Plecoglossus altivelis)

Environmental Monitoring and Assessment, Journal Year: 2024, Volume and Issue: 196(4)

Published: March 16, 2024

Language: Английский

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No attenuation of fish and mammal biodiversity declines in the Guiana Shield

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 971, P. 179021 - 179021

Published: March 1, 2025

Real-time biodiversity monitoring should provide more resolved data to quantify shifts in ecological communities progressively altered by anthropogenic disturbances. Identifying trends requires a rapid and efficient inventory method that enables the collection delivery of high-resolution within short intervals. Using aquatic environmental DNA (eDNA), we investigated spatiotemporal changes fish mammal along Maroni River French Guiana. We compared spatial between two years, separated four-year interval, during which an increase disturbances was observed. To evaluate changes, examined impact these on both taxonomic functional diversity. Our findings revealed that, while did not result major decline, it continued drive alterations community richness. Communities underwent their structure, with experiencing decline extreme traits, lost redundancy generalist functions experienced reduction strategies. In context small-scale disturbances, highlight necessity long-term, short-interval capture reorganisation under stress.

Language: Английский

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