The
Third
U.S.
National
Marine
Environmental
DNA
Workshop
on
June
2–5,
2024
brought
together
researchers,
practitioners,
and
policymakers
to
overview
discuss:
(1)
environmental
(e)DNA
–
defined
as
“DNA
in
the
environment”
a
strategic
national
priority,
(2)
eDNA
tool
readiness
approaches
support
decision-making,
(3)
emerging
technologies,
(4)
plans
for
implementation
adoption
monitoring
ecosystem
assessment
“from
microbes
through
whales”.
Uses
applications
of
have
been
revolutionizing
exploration,
measurement,
biodiversity
across
ecosystems,
including
marine
freshwater
aquatic
environments
emphasized
Workshop,
well
terrestrial
aerial
systems.
featured
launch
new
Aquatic
Strategy
from
White
House
Office
Science
Technology
Policy
(2024),
with
ocean
waters,
all
salinities;
was
developed
using
outputs
taskforce
2022
Second
Workshop.
2.5-day
included
speaker
presentations,
panel
discussions,
networking
tools,
applications,
use,
upscaling,
their
future.
led
into
Capitol
Hill
Ocean
Week
(CHOW)
events
(June
5–6,
2024),
during
World
Week.
Note
that
this
summary
paper
represents
author’s
take-away
view
interpretation,
not
necessarily
overall
views
or
consensus
Government,
Smithsonian
Institution,
workshop
organizers,
speakers.
Veterinary Sciences,
Journal Year:
2024,
Volume and Issue:
11(8), P. 369 - 369
Published: Aug. 12, 2024
In
the
last
few
years,
isolation
and
amplification
of
DNA
or
RNA
from
environment
(eDNA/eRNA)
has
proven
to
be
an
alternative
non-invasive
approach
for
molecular
identification
pathogens
pests
in
beekeeping.
We
have
recently
demonstrated
that
bee
pollen
bread
represent
suitable
biological
material
viral
RNA.
present
study,
we
extracted
total
different
products
(pollen,
n
=
25;
bread,
17;
royal
jelly,
15).
All
samples
were
tested
presence
six
most
common
honey
bee-associated
viruses-Deformed
wing
virus
(DWV),
Acute
paralysis
(ABPV),
Chronic
(CBPV),
Sacbrood
(SBV),
Kashmir
(KBV),
Black
queen
cell
(BQCV)-using
a
reverse
transcription
polymerase
chain
reaction
(RT-PCR).
successfully
detected
records
DWV
(10.5%,
6/57),
four
ABPV
(7.0%,
4/57),
three
Israeli
acute
(IAPV)
(5.3%,
3/57),
two
BQCV
(3.5%,
2/57).
Using
primers,
also
IAPV.
The
obtained
sequences
analyzed
phylogenetic
relationships
with
highly
similar
(megablast)
available
GenBank
database.
Bulgarian
isolates
revealed
high
homology
level
strains
Syria
Turkey.
Moreover,
strain
B
first
time
Bulgaria.
contrast
DWV,
formed
separate
clade
tree.
was
closely
grouped
Russian
isolates,
while
IAPV
its
own
included
China.
conclusion,
study
eRNA
can
used
detection
viruses
products.
method
assist
monitoring
health
status
colonies
at
local,
regional,
even
national
levels.
The
Third
U.S.
National
Marine
Environmental
DNA
Workshop
on
June
2–5,
2024
brought
together
researchers,
practitioners,
and
policymakers
to
overview
discuss:
(1)
environmental
(e)DNA
–
defined
as
“DNA
in
the
environment”
a
strategic
national
priority,
(2)
eDNA
tool
readiness
approaches
support
decision-making,
(3)
emerging
technologies,
(4)
plans
for
implementation
adoption
monitoring
ecosystem
assessment
“from
microbes
through
whales”.
Uses
applications
of
have
been
revolutionizing
exploration,
measurement,
biodiversity
across
ecosystems,
including
marine
freshwater
aquatic
environments
emphasized
Workshop,
well
terrestrial
aerial
systems.
featured
launch
new
Aquatic
Strategy
from
White
House
Office
Science
Technology
Policy
(2024),
with
ocean
waters,
all
salinities;
was
developed
using
outputs
taskforce
2022
Second
Workshop.
2.5-day
included
speaker
presentations,
panel
discussions,
networking
tools,
applications,
use,
upscaling,
their
future.
led
into
Capitol
Hill
Ocean
Week
(CHOW)
events
(June
5–6,
2024),
during
World
Week.
Note
that
this
summary
paper
represents
author’s
take-away
view
interpretation,
not
necessarily
overall
views
or
consensus
Government,
Smithsonian
Institution,
workshop
organizers,
speakers.
