Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection DOI Open Access
Andrea Corral‐Lou, Ana Ramón‐Laca,

Lourdes Alcaraz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Abstract Ecosystem monitoring is a fundamental tool to avert biodiversity loss, gathering valuable information that can be used develop conservation policies, evaluating management outcomes, and guiding science-based decision-making. The Mar Menor costal lagoon (South-East of Spain) has experienced episodes eutrophication due intensive agriculture other human activities, causing mass mortalities marine fauna. In this scenario, crucial evaluate the status fauna flora take appropriate measures. Here, our main objective was assess faunal composition its spatial temporal structure associated with pillars support built recreational well-being facilities along Menor. We capitalised on many sea sponges settle these structures by collecting tissue samples for subsequent extraction filtered environmental DNA (i.e. ‘natural sampler DNA’, nsDNA) in northern southern areas lagoon, both spring summer. metabarcoded all at cytochrome oxidase subunit I gene ( COI ), reliably identified 76 taxa belonging nine different phyla, annelids, poriferans, molluscs cnidarians being richest groups. detected emblematic species threatened status, such as pipefishes (genus Syngnathus ) piddock clam, Pholas dactylus , others known become invasive, ascidians Styela canopus Botrylloides niger barnacle Amphibalanus amphitrite polychaetes Branchiomma bairdi boholense . use common widely distributed natural eDNA samplers allowed us characterise diversity, further emphasizing importance low-cost approach biological communities shallow coastal ecosystems.

Language: Английский

Biofouling sponges as natural eDNA samplers for marine vertebrate biodiversity monitoring DOI Creative Commons
Wang Cai,

Barry MacDonald,

Michelle Korabik

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 946, P. 174148 - 174148

Published: June 19, 2024

Environmental DNA (eDNA) analysis has now become a core approach in marine biodiversity research, which typically involves the collection of water or sediment samples. Yet, recently, filter-feeding organisms have received much attention for their potential role as natural eDNA samplers. While indiscriminate use living 'sampling tools' might some cases raise conservation concerns, there are instances highly abundant sessile may nuisance biofouling on artificial structures. Here we demonstrate how sea sponge species that colonizes moorings world's largest curtain hydroacoustic receivers can powerful collector fish information. By sequencing extracted from Vazella pourtalesii retrieved during routine maintenance, detected 23 and mammals, compared to 19 15 revealed by surface bottom respectively, 28 captured groundfish survey surrounding area, more ecologically impactful involve higher additional costs. Sponge-based inventories proved at least informative those obtained traditional methods, also able detect seasonal differences assemblages. We conclude opportunistic sampling an efficient way document monitor our rapidly changing oceans.

Language: Английский

Citations

4
Characterizing Antarctic fish assemblages using eDNA obtained from marine sponge bycatch specimens DOI Creative Commons
Gert‐Jan Jeunen, Miles D. Lamare, Jennifer A. Devine

et al.

Reviews in Fish Biology and Fisheries, Journal Year: 2023, Volume and Issue: 34(1), P. 221 - 238

Published: Oct. 11, 2023

Abstract International conservation goals have been set to mitigate Southern Ocean ecosystem deterioration, with multiple monitoring programs evaluating progress towards those goals. The scale of continuous through visual observations, however, is challenged by the remoteness area and logistical constraints. Given ecological economic importance Ocean, it imperative that additional biological approaches are explored. Recently, marine sponges, which frequently caught discarded in fisheries, shown naturally accumulate environmental DNA (eDNA). Here, we compare fish eDNA signals from sponge bycatch specimens catch records for nine locations on continental shelf (523.5–709 m) 17 slope (887.5–1611.5 within Ross Sea, Antarctica. We recorded a total 20 fishes, 12 fishes reported as catch, 18 observed eDNA, ten detected both methods. While sampling location was largest contributor variation dataset, obtained significantly higher species richness displayed different composition compared records. Overall, read count correlated more strongly abundance over biomass. Species regional between methods, however signal strength low predictor numbers at level. Our results highlight potential detecting larger fraction community recordings, thereby increasing our knowledge this understudied and, ultimately, aiding efforts.

Language: Английский

Citations

11
eDNA tech tracks lethal jellyfish with CRISPR precision DOI Creative Commons
Maslin Osathanunkul

Ecological Informatics, Journal Year: 2025, Volume and Issue: unknown, P. 103008 - 103008

Published: Jan. 1, 2025

Language: Английский

Citations

0
Evaluation of Mediterranean sponges as natural samplers for environmental DNA (eDNA) DOI Creative Commons
Nicolas Garcia‐Seyda, María García,

Dorian Guillemain

et al.

Open Research Europe, Journal Year: 2025, Volume and Issue: 5, P. 69 - 69

Published: March 17, 2025

Marine sponges have emerged as effective natural samplers of environmental DNA (eDNA), offering a promising alternative for biodiversity monitoring. By filtering large volumes seawater, accumulate eDNA from surrounding communities, potentially enhancing species detection in marine environments where conventional water sampling is limited. In this study, we evaluated the recovery efficiency seven Mediterranean sponge to identify optimal candidates biomonitoring. Axinella verrucosa outperformed other tested species, highlighting its potential long-term assessments. Our results align with previous findings that low microbial abundance (LMA) recover more than high (HMA) reinforcing need targeted selection future studies. Detected fish taxa were all bottom dwelling, supporting relevance monitoring cryptic and benthic habitats. As eDNA-based advances, offers valuable complement surveys, particularly habitats challenging.

Language: Английский

Citations

0
DNA State Influences the Uptake and Persistence of Environmental DNA by Marine Sponge Natural Samplers DOI Creative Commons
Rosalie Dowell, Jamie Craggs, Catherine E. I. Head

et al.

Ecology and Evolution, Journal Year: 2025, Volume and Issue: 15(5)

Published: May 1, 2025

ABSTRACT Marine sponges as natural samplers of environmental DNA (eDNA) are receiving growing attention an untapped source biodiversity data. However, little is known about the state (e.g., cellular or extracellular) that captured by these samples and how this compares to conventional aquatic eDNA samples. Here, we present artificial spiking experiment where in extracellular states was added into tanks containing two sponge species. Aquatic sampler (nsDNA) were collected over 7 days from quantified each sample using quantitative real‐time PCR (qPCR). We found there a significant interaction between type (eDNA nsDNA), with lower detection concentration DNA, compared nsDNA also rate significantly than overall. During methodological testing, inhibition observed both species; prohibitive one Further work investigate degree during metabarcoding important understand its impact on communities resolved methods. Synthesis applications . show may originate subset media, potentially providing more stable picture local communities. Natural provide promising option for hard‐to‐reach environments retrieving data archived samples; however, further optimization required what not well represented widely applied approaches.

Language: Английский

Citations

0
Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge eDNA surveys DOI Creative Commons
Gert‐Jan Jeunen, Miles D. Lamare, Vonda J. Cummings

et al.

Environmental DNA, Journal Year: 2023, Volume and Issue: 5(6), P. 1780 - 1792

Published: Nov. 1, 2023

Abstract The Ross Sea, Antarctica, while largely pristine, is experiencing increased anthropogenic pressures, necessitating enhanced biomonitoring efforts for conservation purposes. Environmental DNA (eDNA) extracted from marine sponges provides a promising approach biodiversity monitoring in remote areas by circumventing the need time‐consuming water filtration. Investigations into efficacy of eDNA signal detection across tree life have yet to be fully explored. Here, we conducted seawater and sponge metabarcoding survey at seven coastal locations Sea assess spatial eukaryote patterns investigate differences between both substrates. In total, detected 1450 operational taxonomic units (OTUs) 30 phyla. Significant richness composition were observed, with partial overlap OTU substrates and, thereby, underscoring crucial role substrate selection surveys. Furthermore, alpha beta diversity analyses revealed distinct signals among sampling locations, which corroborated known species distributions. However, only 135 OTUs (9%) could successfully assigned level, 574 (40%) unable taxonomically classified, due limitations reference database. Our results provide evidence potential areas, demonstrate consider more sophisticated strategies whereby multiple are incorporated, highlight importance complete databases robust taxonomy assignment signals.

Language: Английский

Citations

8
A roadmap for biomonitoring in the 21st century: Merging methods into metrics via ecological networks DOI
Jordan P. Cuff, Mukilan Deivarajan Suresh, Matthew E.G. Dopson

et al.

Advances in ecological research/Advances in Ecological Research, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 34

Published: Jan. 1, 2023

Language: Английский

Citations

7
Monitoring of mammal and bird species in an urban ecological park using environmental DNA metabarcoding DOI
Heejung Sohn, Youngkeun Song

Urban Ecosystems, Journal Year: 2024, Volume and Issue: 27(5), P. 1891 - 1904

Published: May 25, 2024

Language: Английский

Citations

2
Utilizing environmental DNA and imaging to study the deep-sea fish community of Takuyo-Daigo Seamount DOI Creative Commons
Akira Iguchi,

Miyuki Nishijima,

Eri Ikeuchi

et al.

npj Biodiversity, Journal Year: 2024, Volume and Issue: 3(1)

Published: May 31, 2024

Abstract The increase in interest of mining at seamounts means there is a critical need to establish baseline inventories through environmental survey, with the aim promoting conservation and stewardship these remote habitats. To efficiently evaluate fish biodiversity around seamount, we compared DNA (eDNA) methods using seawater sponge samples against imagery obtained remotely operated vehicle (ROV) free-fall deep-sea camera lander called Edokko Mark I on Takuyo-Daigo Seamount (153.0°E, 23.5°N) northwestern Pacific Ocean. We detected total 18 families by methods. fauna seamount included many commonly found areas were similar other located latitudes Pacific. Significant differences patterns detection between eDNA imaging attributed differing powers some groups (related primer compatibility size). For fish, difference composition family level was not significant, but ROV significant; latter likely due whether or bait used attract fish. Although workflow implemented here requires improvements, use combination provided better insight into fishes where our knowledge has been extremely limited. Our recovery from demonstrates potential for facilitating surveys impact assessments activities obtain results previously possible visual only.

Language: Английский

Citations

1
Unlocking Antarctic molecular time-capsules – recovering historical environmental DNA from museum-preserved sponges DOI
Gert‐Jan Jeunen, Sadie Mills, Miles D. Lamare

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 28, 2024

ABSTRACT Marine sponges have recently emerged as efficient natural environmental DNA (eDNA) samplers. The ability of to accumulate eDNA provides an exciting opportunity reconstruct contemporary communities and ecosystems with high temporal spatial precision. However, the use historical (heDNA), trapped within vast number specimens stored in scientific collections, opens up begin past. Here, using a variety Antarctic sponge extensive marine invertebrate collection, we were able recover information on fish biodiversity from 20 years old. We successfully recovered 64 heDNA signals 27 specimens. Alpha diversity measures did not differ among preservation methods, but frozen had significantly different community composition compared those dry or ethanol. Our results show that consistently reliably extract specimens, thereby enabling reconstruction investigation recent past resolution previously unattainable. Future research into extraction other well impact specimen age collection method will strengthen expand opportunities for this novel resource access new knowledge ecological change during last century.

Language: Английский

Citations

0