Building a reliable 16S mini-barcode library of wild bees from Occitania, south-west of France DOI Creative Commons
Anaïs Marquisseau, Kamila Canale‐Tabet, Emmanuelle Labarthe

et al.

Biodiversity Data Journal, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 7, 2025

DNA barcoding and metabarcoding are now powerful tools for studying biodiversity especially the accurate identification of large sample collections belonging to diverse taxonomic groups. Their success depends largely on resolution sequences used as barcodes reliability reference databases. For wild bees, barcode coverage is consistently growing in volume, but some incorrect species annotations need be cared for. The COI (Cytochrome Oxydase subunit 1) gene, most barcoding/metabarcoding arthropods, suffers from primer bias difficulties covering all bee using classical Folmer primers. We present here a curated database 250 bp mini-barcode region 16S rRNA suitable low-cost bees applications, such eDNA analysis or sequencing ancient degraded DNA. Sequenced specimens were captured Occitania (south-west France) morphologically identified by entomologists, with total 530 individuals 171 19 genera. A customised workflow including distance-tree inferences second round entomologist observations, when necessary, was validation 348 mini-barcodes 148 species. Amongst them, 93 did not have any available before our contribution. This high-quality library data freely scientific community, aim facilitating future large-scale characterisation communities context pollinators' decline.

Language: Английский

Building a reliable 16S mini-barcode library of wild bees from Occitania, south-west of France DOI Creative Commons
Anaïs Marquisseau, Kamila Canale‐Tabet, Emmanuelle Labarthe

et al.

Biodiversity Data Journal, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 7, 2025

DNA barcoding and metabarcoding are now powerful tools for studying biodiversity especially the accurate identification of large sample collections belonging to diverse taxonomic groups. Their success depends largely on resolution sequences used as barcodes reliability reference databases. For wild bees, barcode coverage is consistently growing in volume, but some incorrect species annotations need be cared for. The COI (Cytochrome Oxydase subunit 1) gene, most barcoding/metabarcoding arthropods, suffers from primer bias difficulties covering all bee using classical Folmer primers. We present here a curated database 250 bp mini-barcode region 16S rRNA suitable low-cost bees applications, such eDNA analysis or sequencing ancient degraded DNA. Sequenced specimens were captured Occitania (south-west France) morphologically identified by entomologists, with total 530 individuals 171 19 genera. A customised workflow including distance-tree inferences second round entomologist observations, when necessary, was validation 348 mini-barcodes 148 species. Amongst them, 93 did not have any available before our contribution. This high-quality library data freely scientific community, aim facilitating future large-scale characterisation communities context pollinators' decline.

Language: Английский

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