Environmental DNA Transport at an Offshore Mesophotic Bank in the Northwestern Gulf of Mexico
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 27, 2024
Accurately
constraining
the
transport
of
environmental
DNA
(eDNA)
after
it
is
shed
from
an
animal
vital
to
appropriately
geolocate
species
detections
and
biodiversity
measurements
eDNA
sequencing
data.
Modeling
studies
predict
horizontal
at
concentrations
detectable
using
quantitative
PCR
over
scales
tens
kilometers,
but
more
limited
vertical
transport.
Field
routinely
find
that
metabarcoding
data
distinguishes
biological
communities
small
spatial
scales,
meters.
Here,
we
leverage
unique
bathymetry
offshore,
mesophotic
bank
benthic
invertebrate
community
supports
determine
extent
which
may
affect
interpretation
We
found
in
a
stratified
water
column,
invertebrates
was
vertically
constrained
depths
close
seafloor
thermocline
versus
surface
mixed
layer
above.
However,
when
primers
are
taxonomically
specific
corals,
evidence
for
coral
distances
least
~1.5
km
where
they
can
be
reasonably
expected
occur.
On
contrary,
there
minimal
generated
broadly
targeted
sequences
eukaryotes.
These
results
highlight
importance
as
well
considering
methodological
details,
like
taxonomic
specificity
primers,
interpreting
Language: Английский
Continual Day–Night eDNA Detectability Amidst Diel Reef Species Fluctuations on Diver Transects
Environmental DNA,
Journal Year:
2024,
Volume and Issue:
6(5)
Published: Sept. 1, 2024
ABSTRACT
Recent
research
into
the
spatiotemporal
dynamics
of
eDNA
in
marine
environments
indicates
that
signals
are
highly
localized
and
may
dissipate
beyond
detection
levels
within
a
few
hours
production.
This
affects
whether
single‐timepoint
sampling,
which
generally
occurs
during
daylight
hours,
or
cyclic
(day/night)
interval
sampling
is
necessary
to
detect
both
diurnal
nocturnal
species.
Our
study
investigated
short‐term
variability
derived
from
fishes
macroinvertebrates
across
three
temperate
reef
sites
eastern
Tasmania,
Australia.
Simultaneous
underwater
visual
census
(UVC)
diver
transect
surveys
were
conducted
every
6
h
over
24‐h
period
investigate
was
able
species
outside
their
UVC‐observed
diel
activity.
We
report
can
on
reefscapes.
A
lack
compositional
turnover
between
day
night
suggests
persists
12
and/or
continuously
produced
by
taxa,
irrespective
behavioral
changes
observed
UVC.
Given
high
sample
variability,
however,
we
recommend
replication
level
(>
10
×
1
L
samples)
produce
robust
site
community
composition
profiles.
builds
emerging
literature
eDNA,
assisting
design
future
studies
at
with
pronounced
variation
faunal
activity
night.
Language: Английский