Continual Day–Night eDNA Detectability Amidst Diel Reef Species Fluctuations on Diver Transects DOI Creative Commons
Katrina M. West, Tyson R. Jones, Lara Denis‐Roy

et al.

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(5)

Published: Sept. 1, 2024

ABSTRACT Recent research into the spatiotemporal dynamics of eDNA in marine environments indicates that signals are highly localized and may dissipate beyond detection levels within a few hours production. This affects whether single‐timepoint sampling, which generally occurs during daylight hours, or cyclic (day/night) interval sampling is necessary to detect both diurnal nocturnal species. Our study investigated short‐term variability derived from fishes macroinvertebrates across three temperate reef sites eastern Tasmania, Australia. Simultaneous underwater visual census (UVC) diver transect surveys were conducted every 6 h over 24‐h period investigate was able species outside their UVC‐observed diel activity. We report can on reefscapes. A lack compositional turnover between day night suggests persists 12 and/or continuously produced by taxa, irrespective behavioral changes observed UVC. Given high sample variability, however, we recommend replication level (> 10 × 1 L samples) produce robust site community composition profiles. builds emerging literature eDNA, assisting design future studies at with pronounced variation faunal activity night.

Language: Английский

Environmental DNA Transport at an Offshore Mesophotic Bank in the Northwestern Gulf of Mexico DOI Open Access
Luke McCartin, Annette F. Govindarajan, Jill M. McDermott

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 27, 2024

Accurately constraining the transport of environmental DNA (eDNA) after it is shed from an animal vital to appropriately geolocate species detections and biodiversity measurements eDNA sequencing data. Modeling studies predict horizontal at concentrations detectable using quantitative PCR over scales tens kilometers, but more limited vertical transport. Field routinely find that metabarcoding data distinguishes biological communities small spatial scales, meters. Here, we leverage unique bathymetry offshore, mesophotic bank benthic invertebrate community supports determine extent which may affect interpretation We found in a stratified water column, invertebrates was vertically constrained depths close seafloor thermocline versus surface mixed layer above. However, when primers are taxonomically specific corals, evidence for coral distances least ~1.5 km where they can be reasonably expected occur. On contrary, there minimal generated broadly targeted sequences eukaryotes. These results highlight importance as well considering methodological details, like taxonomic specificity primers, interpreting

Language: Английский

Citations

0
Continual Day–Night eDNA Detectability Amidst Diel Reef Species Fluctuations on Diver Transects DOI Creative Commons
Katrina M. West, Tyson R. Jones, Lara Denis‐Roy

et al.

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(5)

Published: Sept. 1, 2024

ABSTRACT Recent research into the spatiotemporal dynamics of eDNA in marine environments indicates that signals are highly localized and may dissipate beyond detection levels within a few hours production. This affects whether single‐timepoint sampling, which generally occurs during daylight hours, or cyclic (day/night) interval sampling is necessary to detect both diurnal nocturnal species. Our study investigated short‐term variability derived from fishes macroinvertebrates across three temperate reef sites eastern Tasmania, Australia. Simultaneous underwater visual census (UVC) diver transect surveys were conducted every 6 h over 24‐h period investigate was able species outside their UVC‐observed diel activity. We report can on reefscapes. A lack compositional turnover between day night suggests persists 12 and/or continuously produced by taxa, irrespective behavioral changes observed UVC. Given high sample variability, however, we recommend replication level (> 10 × 1 L samples) produce robust site community composition profiles. builds emerging literature eDNA, assisting design future studies at with pronounced variation faunal activity night.

Language: Английский

Citations

0