A qPCR assay for the detection ofPhytophthora abietivora, an emerging pathogen on fir species cultivated as Christmas trees DOI Creative Commons
Guillaume Charron, Marie‐Krystel Gauthier, Hervé Van der Heyden

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

Abstract Emerging species of the Phytophthora genus are among most important threats to global plant biodiversity. For instance, root rot (PRR) Christmas trees is responsible for 10% observed mortality rate in nurseries. Diagnosis PRR involves isolation followed by morphological and molecular identification causal agents. However, these methods rarely adapted larger scale experiments such as situ detection. applications, detection environmental DNA (eDNA) provides high-throughput fast result generation needed. abietivora was associated firs cultivated province Québec (Canada). This study focused on developing a sensitive specific qPCR assay targeting P. validating its efficiency eDNA samples. A set primers probe designed this assay, parameters limit (LoD 95% ) quantification (LoQ) were measured. The tested obtained from healthy-looking symptomatic firs. shown be semi-specific because it cross-reacted with abietivora, four phylogenetically close unrelated fir diseases. (LOD estimated at 10 copies per reaction (C q 35.7). showed reliable down 33 oospores gram soil. Out 488 samples soil, 68 positive abietivora. While factors tree species, sampled region or year sampling did not affect proportion results, showing PRR-like symptoms had significantly higher odds testing compared trees. will useful rapid diagnostics infected prospecting tool better characterize natural distribution dissemination disease.

Language: Английский

Oomycete communities are influenced by land use and disease status in Christmas tree production in Southern Québec, Canada DOI Creative Commons
Hervé Van der Heyden, Marc‐Olivier Duceppe, Guillaume Charron

et al.

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(2)

Published: March 1, 2024

Abstract Forests are threatened by many natural stressors intensified climate change and anthropogenic activities, which tend to increase their susceptibility pests pathogens. Consequently, oomycete‐related forest decline or dieback cases increasing in natural, urban, agricultural landscapes. It is this context that Christmas tree growers from Southern Québec, Canada, experiencing root rot problems, with reported incidences up 25%. In a previous study, seven Phytophthora spp. were associated problem, but the overall diversity of oomycetes has not yet been investigated. Hence, we use metabarcoding approach provide an overview diversity, richness, composition oomycete community fir plantations compared surrounding forests. We showed P. cryptogea cluster, europaea sansomeana , chlamydospora cluster significantly more abundant soils collected under diseased trees confirmed (including abietivora ) was most frequently showing rot‐like symptoms. Finally, report land (anthropogenic activities) shapes while can act as gateway for invading fact, results presented here suggested might already have crossed boundary other species follow, advocating importance improved surveillance various environments.

Language: Английский

Citations

3
A qPCR assay for the detection of Phytophthora abietivora, an emerging pathogen on fir species cultivated as Christmas trees DOI Creative Commons
Guillaume Charron, Marie‐Krystel Gauthier, Hervé Van der Heyden

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0320680 - e0320680

Published: April 2, 2025

Emerging species of the Phytophthora genus are among most important threats to global plant biodiversity, horticulture and trade. For instance, root rot (PRR) Christmas trees, mainly Fraser ( Abies fraseri ) balsam balsamea firs, is responsible for an average 10% observed diseased trees in plantations. Diagnosing PRR involves isolation followed by morphological molecular identification causal agents. However, these methods rarely adapted larger scale monitoring such as situ detection. applications, detection environmental DNA (eDNA) provides fast high-throughput results needed. abietivora was associated with firs spp.) cultivated province Québec (Canada). This study focused on developing a sensitive specific qPCR assay targeting P. validating its efficiency extracted from soil roots. A set primers probe designed this assay, parameters limit (LOD 95% quantification (LOQ) were measured. The tested obtained healthy-looking symptomatic firs. shown be semi-specific because it cross-reacted europaea three other phylogenetically close species, but deemed context LOD estimated at 10 copies per reaction (C q 35.7) LOQ 33 oospores gram soil. Out 488 samples soil, 68 positive which 42 (61.7%) trees. Only slight overlap (3 out 7 samples) previously baiting . will useful rapid diagnostics infected prospecting tool better characterize natural distribution dissemination disease.

Language: Английский

Citations

0
A qPCR assay for the detection ofPhytophthora abietivora, an emerging pathogen on fir species cultivated as Christmas trees DOI Creative Commons
Guillaume Charron, Marie‐Krystel Gauthier, Hervé Van der Heyden

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

Abstract Emerging species of the Phytophthora genus are among most important threats to global plant biodiversity. For instance, root rot (PRR) Christmas trees is responsible for 10% observed mortality rate in nurseries. Diagnosis PRR involves isolation followed by morphological and molecular identification causal agents. However, these methods rarely adapted larger scale experiments such as situ detection. applications, detection environmental DNA (eDNA) provides high-throughput fast result generation needed. abietivora was associated firs cultivated province Québec (Canada). This study focused on developing a sensitive specific qPCR assay targeting P. validating its efficiency eDNA samples. A set primers probe designed this assay, parameters limit (LoD 95% ) quantification (LoQ) were measured. The tested obtained from healthy-looking symptomatic firs. shown be semi-specific because it cross-reacted with abietivora, four phylogenetically close unrelated fir diseases. (LOD estimated at 10 copies per reaction (C q 35.7). showed reliable down 33 oospores gram soil. Out 488 samples soil, 68 positive abietivora. While factors tree species, sampled region or year sampling did not affect proportion results, showing PRR-like symptoms had significantly higher odds testing compared trees. will useful rapid diagnostics infected prospecting tool better characterize natural distribution dissemination disease.

Language: Английский

Citations

0