Detecting Threatened Ichthyofauna in a Mediterranean Intensive Agricultural Landscape: From DNA Traces to Electrofishing DOI
Panagiota Xanthopoulou, Argyrios Sapounidis, Paraskevi Papadopoulou

et al.

Aquatic Conservation Marine and Freshwater Ecosystems, Journal Year: 2025, Volume and Issue: 35(4)

Published: April 1, 2025

ABSTRACT Intensive agriculture and farming activities, industry, as well human settlement can seriously affect freshwater ecosystems the species they host. These disturbances have been previously monitored using traditional catch surveys. New methods, such environmental DNA (eDNA) metabarcoding, significantly improve biodiversity mapping by accessing rarely visited areas enhancing detection of rare invasive species. We conducted an eDNA survey in a complex river tributary system at Tenagi, Philippi that hosts approximately 12.5% Greek ichthyofauna. A primer pair targeting 12S rRNA was used to analyse samples from 30 sites. reference sequence database generated regional diversity monitoring endemic, native fishes. Results were compared with data collected electrofishing stations allowed access. Two three invasive, two translocated 10 detected their current known distribution both methods. Overall, metabarcoding being more sensitive, consistently than methods on single fieldwork expedition. Co‐occurrence also investigated for first time, revealing minor variations composition within fish communities. Stations intensive agricultural zones potentially high accumulation organic inorganic pollutants (Tributary D), exhibited lower number reads richness other tributaries Tenagi. Our results demonstrate need complementary use assess assemblages habitats, combined approach address reliability issues biases associated each method.

Language: Английский

Temporal and Spatial Dynamics of the Lotic Fish Communities: A Comparison of Coffee Filter‐Based Passive eDNA Collection Versus Active eDNA Filtering DOI Creative Commons
Jelena Mlinarec,

Ida Svetličić,

Matija Kresonja

et al.

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(2)

Published: March 1, 2025

ABSTRACT Environmental DNA (eDNA) metabarcoding is a noninvasive and cost‐effective method for fish community monitoring, enabling the determination of both presence/absence species their relative quantity in closed systems. However, integration these novel eDNA‐based methods into current monitoring practices not straightforward require standardization methodological approaches. In this study, we investigated spatial temporal variation assemblages within two lotic systems Croatia. Six sampling stations were located alongside middle section river Sava upstream downstream Zagreb, one location stream Okićnica. We compared traditional field surveys with eDNA methodologies: active filtering passive collection. showed that collection using coffee filters detected composition as effectively filtration, providing comparable results terms local richness assemblages. Generally, our approach greater per site than electrofishing. Each single sample captured an average 18.3 species, from total 30 encountered 78 samples. The sites Zagreb significant differences read abundance comparison to downstream. Nonmetric multidimensional scaling (nMDS) plot based on appeared be structured according type system, clear separation two‐dimensional space between samples Okićnica seasons. A substantial increase during spawning periods certain was observed, emphasizing method's utility unraveling ecological complexities. Altogether, study exemplifies how powerful tool standardized information will valuable environmental management.

Language: Английский

Citations

0
Detecting Threatened Ichthyofauna in a Mediterranean Intensive Agricultural Landscape: From DNA Traces to Electrofishing DOI
Panagiota Xanthopoulou, Argyrios Sapounidis, Paraskevi Papadopoulou

et al.

Aquatic Conservation Marine and Freshwater Ecosystems, Journal Year: 2025, Volume and Issue: 35(4)

Published: April 1, 2025

ABSTRACT Intensive agriculture and farming activities, industry, as well human settlement can seriously affect freshwater ecosystems the species they host. These disturbances have been previously monitored using traditional catch surveys. New methods, such environmental DNA (eDNA) metabarcoding, significantly improve biodiversity mapping by accessing rarely visited areas enhancing detection of rare invasive species. We conducted an eDNA survey in a complex river tributary system at Tenagi, Philippi that hosts approximately 12.5% Greek ichthyofauna. A primer pair targeting 12S rRNA was used to analyse samples from 30 sites. reference sequence database generated regional diversity monitoring endemic, native fishes. Results were compared with data collected electrofishing stations allowed access. Two three invasive, two translocated 10 detected their current known distribution both methods. Overall, metabarcoding being more sensitive, consistently than methods on single fieldwork expedition. Co‐occurrence also investigated for first time, revealing minor variations composition within fish communities. Stations intensive agricultural zones potentially high accumulation organic inorganic pollutants (Tributary D), exhibited lower number reads richness other tributaries Tenagi. Our results demonstrate need complementary use assess assemblages habitats, combined approach address reliability issues biases associated each method.

Language: Английский

Citations

0