Guidelines for study the oxidative stress damage and mechanism of on Caenorhabditis elegans DOI Creative Commons

Shengxiong Chen,

Peng Zhou, Rui Lü

et al.

Future Postharvest and Food, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract Oxidative stress, caused by free radicals damaging cellular structures, is associated with various diseases. Effective research on oxidative stress mechanisms and potential therapies requires robust model organisms. Caenorhabditis elegans ( C. ) an ideal due to its small size, high transparency, ease of cultivation. Additionally, exhibits human‐like responses under such as changes in antioxidant enzyme activity, accumulation damage, regulation antioxidant‐related gene expression. This guideline outlines methods for utilizing research, detailing the construction models corresponding detection indicators. comprehensive approach aims advance study evaluation antioxidants.

Language: Английский

Preparation of low molecular weight polysaccharides from Tremella fuciformis by ultrasonic-assisted H2O2-Vc method: Structural characteristics, in vivo antioxidant activity and stress resistance DOI Creative Commons

Quancen Lee,

Xianjing Han,

Mingfeng Zheng

et al.

Ultrasonics Sonochemistry, Journal Year: 2023, Volume and Issue: 99, P. 106555 - 106555

Published: Aug. 9, 2023

Different methods were used to degrade Tremella fuciformis polysaccharides (TFP) and prepare low molecular weight of (TFLP) improve their bioavailability. It was found that the TFLP prepared by ultrasonic-assisted H2O2-Vc method showed highest level antioxidant activity stress resistance in C. elegans. The structural characteristics, vivo TFLP-1 evaluated after isolation purification TFLP, it an acid polysaccharide with a 75770 Da, which mainly composed mannose. Meanwhile, could regulate elegans upregulating transcription fat-5, fat-7, acs-2, glp-1, hsf-1, hsp-1, mtl-1, nhr-49, skn-1 sod-3 mRNA. improvement effects closely related significant regulation galactose metabolism, alpha linolenic pantothenate CoA biosynthesis metabolic pathways. These results provided insights into high value application food industry development functional foods.

Language: Английский

Citations

22
Antioxidant activity of low molecular weight polysaccharides from Tremella fuciformis in Caenorhabditis elegans based on metabolomics analysis DOI Creative Commons

Quancen Lee,

Zhixiang Xue,

Mingfeng Zheng

et al.

Journal of Future Foods, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Protein-rich yeast extract (®fermgard) has potential antioxidant and anti-aging activities DOI

Quancen Li,

Wenwen Cai,

Na Li

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2023, Volume and Issue: 270, P. 109656 - 109656

Published: May 15, 2023

Language: Английский

Citations

2
Guidelines for study the oxidative stress damage and mechanism of on Caenorhabditis elegans DOI Creative Commons

Shengxiong Chen,

Peng Zhou, Rui Lü

et al.

Future Postharvest and Food, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract Oxidative stress, caused by free radicals damaging cellular structures, is associated with various diseases. Effective research on oxidative stress mechanisms and potential therapies requires robust model organisms. Caenorhabditis elegans ( C. ) an ideal due to its small size, high transparency, ease of cultivation. Additionally, exhibits human‐like responses under such as changes in antioxidant enzyme activity, accumulation damage, regulation antioxidant‐related gene expression. This guideline outlines methods for utilizing research, detailing the construction models corresponding detection indicators. comprehensive approach aims advance study evaluation antioxidants.

Language: Английский

Citations

0