Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)¹

Autophagy, Journal Year: 2021, Volume and Issue: 17(1), P. 1 - 382

Published: Jan. 2, 2021

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular eukaryotes. Here, present investigators select interpret examine related processes, reviewers provide realistic reasonable critiques reports that are focused these processes. These not meant dogmatic rules, because appropriateness any assay largely depends question being asked system used. Moreover, no individual perfect every situation, calling use multiple techniques properly monitor each experimental setting. Finally, several core components machinery implicated distinct autophagic processes (canonical noncanonical autophagy), implying genetic approaches block should rely targeting two or more autophagy-related genes ideally participate steps pathway. Along similar lines, proteins involved regulate other cellular pathways including apoptosis, all them can used as specific marker bona fide responses. critically discuss current assessing information they can, cannot, provide. ultimate goal encourage intellectual technical innovation

Language: Английский

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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

European Journal of Immunology, Journal Year: 2019, Volume and Issue: 49(10), P. 1457 - 1973

Published: Oct. 1, 2019

These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed. Furthermore, tips, tricks pitfalls avoid, written peer-reviewed by leading experts field, making this an essential research companion.

Language: Английский

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Peptide-specific recognition of human cytomegalovirus strains controls adaptive natural killer cells

Nature Immunology, Journal Year: 2018, Volume and Issue: 19(5), P. 453 - 463

Published: April 9, 2018

Language: Английский

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Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

European Journal of Immunology, Journal Year: 2021, Volume and Issue: 51(12), P. 2708 - 3145

Published: Dec. 1, 2021

Abstract The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes functional assays all major human murine immune cell subsets. Notably, contain helpful tables highlighting differences between cells. Another useful feature this is analysis clinical samples with examples applications in context autoimmune diseases, cancers as well acute chronic infectious diseases. Furthermore, there are detailing tips, tricks pitfalls avoid. All written peer‐reviewed by leading experts immunologists, making an essential state‐of‐the‐art handbook for basic researchers.

Language: Английский

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Interleukin-22 protects intestinal stem cells against genotoxic stress

Nature, Journal Year: 2019, Volume and Issue: 566(7743), P. 249 - 253

Published: Jan. 30, 2019

Language: Английский

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Current gaps in sepsis immunology: new opportunities for translational research

The Lancet Infectious Diseases, Journal Year: 2019, Volume and Issue: 19(12), P. e422 - e436

Published: Oct. 17, 2019

Language: Английский

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Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence

Molecular Cell, Journal Year: 2019, Volume and Issue: 76(1), P. 110 - 125.e9

Published: Aug. 29, 2019

Language: Английский

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Microbiota-derived peptide mimics drive lethal inflammatory cardiomyopathy

Science, Journal Year: 2019, Volume and Issue: 366(6467), P. 881 - 886

Published: Nov. 15, 2019

Peptide mimicry breaks the heart Myocarditis, a prolonged chronic inflammation of muscle, can eventually progress to inflammatory cardiomyopathy, serious condition associated with failure. Activated T helper (T H ) cells that recognize myosin heavy chain 6–derived peptides are thought play central role in this pathogenesis. Using mouse model myocarditis, Gil-Cruz et al. found cardiac myosin–reactive initially primed by myosin-peptide mimics derived from commensal Bacteroides species gut (see Perspective Epelman). Unlike heathy controls, human myocarditis patients also showed detectable immune reactivity both and antigens. Treatment antibiotics dampened responses prevented lethal disease. Science , issue p. 881 ; see 806

Language: Английский

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Advances in Cryopreservation of Bull Sperm

Frontiers in Veterinary Science, Journal Year: 2019, Volume and Issue: 6

Published: Aug. 27, 2019

Cryopreservation of semen and artificial insemination have an important, positive impact on cattle production product quality. Through the use cryopreserved insemination, sperm from best breeding bulls can be used to inseminate thousands cows around world. Although cryopreservation bull has advanced beyond that other species, there are still major gaps in knowledge technology bases. Post-thaw viability is low differs significantly among bulls. These weaknesses important because they preventing advances both fundamental science mammalian gametes reproductive biotechnology. Various extenders been developed supplemented with chemicals reduce cryodamage or oxidative stress varying levels success. More detailed insights morphology function uncovered through application tools modern molecular cell biology. This article provides a concise review progress sperm, extender development, frontiers using diverse techniques study viability. scientific resource animal biotechnology discovery fertility markers, urgent need improve post-thaw enhanced for precision agriculture produce food animals ensure security global scale.

Language: Английский

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The Skin Commensal Yeast Malassezia Triggers a Type 17 Response that Coordinates Anti-fungal Immunity and Exacerbates Skin Inflammation

Cell Host & Microbe, Journal Year: 2019, Volume and Issue: 25(3), P. 389 - 403.e6

Published: March 1, 2019

Language: Английский

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