Flow Rate Determination of the Nanoflow Sheath Liquid CE–MS‐Coupling Applying the nanoCEasy Interface DOI Creative Commons
Alisa Höchsmann, Jasmin Schairer,

O. Schott

et al.

Electrophoresis, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

ABSTRACT In recent years, nanoflow sheath liquid (nanoSL) interfaces have been used more commonly for capillary electrophoresis–mass spectrometry (CE–MS) coupling due to their high sensitivity combined with flexibility in CE separation conditions. So far, the exact amount of (SL) and, thus, dilution effect remain unknown self‐supplying type nanoSL interfaces. To quantify SL flow rates, we present here an approach determination rate based on isotopically labeled standards using nanoCEasy interface. and pumped are spiked atrazine d5‐atrazine, respectively, enabling total different electrospray (ES) Generally, rates increase higher ES voltages. Only at voltages, larger emitter sizes lead rates; low independent size. Additionally, smaller can generally be operated lower Visualizing ES, a spray angle. Higher may decrease signal intensity by diluting CE‐separated analytes. Indeed, this is observed flow‐injected analytes CE–MS analysis amino acid standard mix under aqueous acidic Therefore, preferred electroosmotic (EOF) applications. However, EOF applications, voltages required prevent accumulation background electrolyte (BGE) tip, which otherwise leads peak broadening inefficient ionization. Overall, presented data enable selection stable sensitive settings

Language: Английский

Coupling Capillary Electrophoresis With a Shifted Inlet Potential High‐Resolution Ion Mobility Spectrometer DOI Creative Commons

Klaus Welters,

Christian Thoben, Christian‐Robert Raddatz

et al.

Electrophoresis, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

ABSTRACT We present the coupling of capillary electrophoresis to a custom‐built high‐resolution ion mobility spectrometer (IMS). This system integrates shifted inlet potential IMS configuration with customised nanoflow ESI sheath interface. It enables rapid analysis quaternary ammonium compounds (QACs) and their impurities in real‐world samples. allowed detection six non‐chromophoric about 3 min. The assignment signals was supported by matching experimentally determined collision cross‐section (CCS) values predicted values. achieved limit single‐digit picogram range resolutions over 80.

Language: Английский

Citations

0
Trendbericht Analytische Chemie 2024 DOI Open Access
Günter Gauglitz, Heiko Hayen,

Martin Jäger

et al.

Nachrichten aus der Chemie, Journal Year: 2024, Volume and Issue: 72(4), P. 52 - 64

Published: March 29, 2024

Abstract Mehrdimensionale Trenntechniken sowie Kopplungstechniken verbessern weiter die Auflösung in der Analytik. In Elementanalytik beeindrucken neue Entwicklungen Massenspektrometrie mit induktiv gekoppeltem Plasma, Archäometrie zerstörungsfreie In‐situ‐Analysen. Der 3‐D‐Druck treibt elektroanalytische Forschung voran, und Prozessanalytik setzt auf Lab‐on‐a‐Chip vermeldet Neues bei Raman‐Spektroskopie Flüssigkernlichtleitern.

Citations

1
Flow Rate Determination of the Nanoflow Sheath Liquid CE–MS‐Coupling Applying the nanoCEasy Interface DOI Creative Commons
Alisa Höchsmann, Jasmin Schairer,

O. Schott

et al.

Electrophoresis, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

ABSTRACT In recent years, nanoflow sheath liquid (nanoSL) interfaces have been used more commonly for capillary electrophoresis–mass spectrometry (CE–MS) coupling due to their high sensitivity combined with flexibility in CE separation conditions. So far, the exact amount of (SL) and, thus, dilution effect remain unknown self‐supplying type nanoSL interfaces. To quantify SL flow rates, we present here an approach determination rate based on isotopically labeled standards using nanoCEasy interface. and pumped are spiked atrazine d5‐atrazine, respectively, enabling total different electrospray (ES) Generally, rates increase higher ES voltages. Only at voltages, larger emitter sizes lead rates; low independent size. Additionally, smaller can generally be operated lower Visualizing ES, a spray angle. Higher may decrease signal intensity by diluting CE‐separated analytes. Indeed, this is observed flow‐injected analytes CE–MS analysis amino acid standard mix under aqueous acidic Therefore, preferred electroosmotic (EOF) applications. However, EOF applications, voltages required prevent accumulation background electrolyte (BGE) tip, which otherwise leads peak broadening inefficient ionization. Overall, presented data enable selection stable sensitive settings

Language: Английский

Citations

0