Regulation of Protein Transport in Functionalized PET Nanopores DOI
Jilie Kong,

Rana Jahani,

Haiyan Zheng

et al.

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Facilitated translocation is a critical mechanism for transporting substances in biological systems, where molecular and ionic species move across the membrane with help of specific transmembrane protein ion channels. In this work, we systematically examined transport three poly(ethylene terephthalate) (PET) nanopores modified different types surface functions (hydroxyl, phenyl, amine). We found that event signature as well kinetics thermodynamics movement PET nanopore varied significantly change function pore. addition to electrophoretic effect, other factors such diffusion, electro-osmotic selectivity channel, affinity strength between functional group also play significant roles transport. Although properly functionalized individual can be used stochastic elements rapid differentiation characterization, enhanced resolution accuracy could accomplished by employing an array having inner groups characterize proteins based on their collective responses. Given important living organisms applications biomarkers early disease diagnosis prognosis, pattern-recognition solid-state nanopore-sensing strategy detection characterization developed work may find useful various fields.

Language: Английский

Protease-Hydrolysis-Driven Approach towards the Quantification of Cellular mRNA after Drug Treatment in Protein Nanopores DOI

Ling Zheng,

Jizhen Lin,

W. W. Tian

et al.

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 343955 - 343955

Published: March 1, 2025

Language: Английский

Citations

0
Regulation of Protein Transport in Functionalized PET Nanopores DOI
Jilie Kong,

Rana Jahani,

Haiyan Zheng

et al.

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Facilitated translocation is a critical mechanism for transporting substances in biological systems, where molecular and ionic species move across the membrane with help of specific transmembrane protein ion channels. In this work, we systematically examined transport three poly(ethylene terephthalate) (PET) nanopores modified different types surface functions (hydroxyl, phenyl, amine). We found that event signature as well kinetics thermodynamics movement PET nanopore varied significantly change function pore. addition to electrophoretic effect, other factors such diffusion, electro-osmotic selectivity channel, affinity strength between functional group also play significant roles transport. Although properly functionalized individual can be used stochastic elements rapid differentiation characterization, enhanced resolution accuracy could accomplished by employing an array having inner groups characterize proteins based on their collective responses. Given important living organisms applications biomarkers early disease diagnosis prognosis, pattern-recognition solid-state nanopore-sensing strategy detection characterization developed work may find useful various fields.

Language: Английский

Citations

0