Unravelling the molecular mechanisms of fish salinity adaptation in the face of multiple stressors: A comparative multi-tissue transcriptomic study in the Llobregat River, Barcelona, Spain DOI Creative Commons
Camilo Escobar-Sierra, Miguel Cañedo‐Argüelles, Dolors Vinyoles

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 20, 2023

Abstract Freshwater salinization poses a growing global environmental concern, introducing complex chemical cocktails and jeopardizing freshwater biodiversity, particularly fish populations. This research aimed to elucidate the molecular foundations of salinity adaptation in non-native minnow species ( Phoxinus septimaniae x P. dragarum ) exposed saline effluents from potash mines Llobregat River, Barcelona, Spain. Employing high-throughput mRNA sequencing differential gene expression analyses, we examined brain, gills, liver tissues collected at two stations (upstream downstream effluent discharge). Salinization markedly influenced profiles, with brain exhibiting most differentially expressed genes, emphasizing its unique sensitivity fluctuations. Pathway analyses revealed expected enrichment ion transport osmoregulation pathways across all tissues. Furthermore, tissue-specific associated stress, reproduction, growth, immune responses, methylation, neurological development were identified context salinization. Rigorous validation RNA-seq data through quantitative PCR (qPCR) underscored robustness consistency our findings platforms. investigation unveils intricate mechanisms steering minnows confronting diverse stressors. Advancing comprehension genomic responses changes, study provides crucial insights into adaptive strategies aquatic organisms grappling comprehensive analysis sheds light on underlying genetic physiological governing salinity-stressed environments, offering essential knowledge for conservation management ecosystems facing escalating pressures.

Language: Английский

Studying interactions among anthropogenic stressors in freshwater ecosystems: A systematic review of 2396 multiple‐stressor experiments DOI Creative Commons
James Orr, Samuel J. Macaulay, Adriana Mordente

et al.

Ecology Letters, Journal Year: 2024, Volume and Issue: 27(6)

Published: June 1, 2024

Abstract Understanding the interactions among anthropogenic stressors is critical for effective conservation and management of ecosystems. Freshwater scientists have invested considerable resources in conducting factorial experiments to disentangle stressor by testing their individual combined effects. However, diversity systems studied has hindered previous syntheses this body research. To overcome challenge, we used a novel machine learning framework identify relevant studies from over 235,000 publications. Our synthesis resulted new dataset 2396 multiple‐stressor freshwater systems. By summarizing methods these studies, quantifying trends popularity investigated stressors, performing co‐occurrence analysis, produce most comprehensive overview diverse field research date. We provide both taxonomy grouping 909 into 31 classes an open‐source interactive version ( https://jamesaorr.shinyapps.io/freshwater‐multiple‐stressors/ ). Inspired our results, help clarify whether statistical detected align with interest, outline general guidelines design any system. conclude highlighting directions required better understand ecosystems facing multiple stressors.

Language: Английский

Citations

16
Single and combined exposure to ‘bee safe’ pesticides alter behaviour and offspring production in a ground-nesting solitary bee ( Xenoglossa pruinosa ) DOI Creative Commons
Sabrina Rondeau, Nigel E. Raine

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 291(2019)

Published: March 20, 2024

Mounting evidence supporting the negative impacts of exposure to neonicotinoids on bees has prompted registration novel ‘bee-friendly’ insecticides for agricultural use. Flupyradifurone (FPF) is a butenolide insecticide that shares same mode action as and been assessed be ‘practically non-toxic adult honeybees' using current risk assessment procedures. However, these assessments overlook some routes specific wild bees, such contact with residues in soil ground-nesters. Co-exposure other pesticides may also lead detrimental synergistic effects. In fully crossed experiment, we possible lethal sublethal effects chronic two used Cucurbita crops, Sivanto Prime fungicide Quadris Top (azoxystrobin difenoconazole), alone or combined, solitary ground-nesting squash ( Xenoglossa pruinosa ). Squash exposed collected less pollen per flower visit, while Sivanto-exposed produced larger offspring. Pesticide co-exposure induced hyperactivity female relative both control single pesticide exposure, reduced number emerging offspring nest compared individual treatments. This study demonstrates ‘low-toxicity’ can adversely affect under field-realistic combination.

Language: Английский

Citations

13
Reconciling Variability in Multiple Stressor Effects Using Environmental Performance Curves DOI Creative Commons
Hebe Carmichael,

Ruth Warfield,

Gabriel Yvon‐Durocher

et al.

Ecology Letters, Journal Year: 2025, Volume and Issue: 28(1)

Published: Jan. 1, 2025

Understanding the effects of multiple stressors has become a major focus in ecology and evolution. While many studies have investigated combined stressors, revealing massive variability, mechanistic understanding that reconciles diversity stressor outcomes is lacking. Here, we show how performance curves can fill this gap by mechanisms shape outcomes. Our experiments with 12 bacterial taxa, demonstrate additional alter temperature, pH salinity curves. This leads to changes interaction outcomes-for example, shifts between additive, antagonistic or synergistic interactions-along gradients, small along nonlinear dramatically impact interaction. These findings help explain lack generality found across highlight curve approach provide more holistic view interactions.

Language: Английский

Citations

1
A synthesis of anthropogenic stress effects on emergence-mediated aquatic-terrestrial linkages and riparian food webs DOI
Ralf Schulz, Mirco Bundschuh, Martin H. Entling

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 908, P. 168186 - 168186

Published: Oct. 30, 2023

Language: Английский

Citations

17
Unraveling the molecular mechanisms of fish physiological response to freshwater salinization: A comparative multi-tissue transcriptomic study in a river polluted by potash mining DOI Creative Commons
Camilo Escobar-Sierra, Miguel Cañedo‐Argüelles, Dolors Vinyoles

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 357, P. 124400 - 124400

Published: June 19, 2024

Freshwater salinization is an escalating global environmental issue that threatens freshwater biodiversity, including fish populations. This study aims to uncover the molecular basis of salinity physiological responses in a non-native minnow species (Phoxinus septimaniae x P. dragarum) exposed saline effluents from potash mines Llobregat River, Barcelona, Spain. Employing high-throughput mRNA sequencing and differential gene expression analyses, brain, gills, liver tissues collected at two stations (upstream downstream effluent discharge) were examined. Salinization markedly influenced profiles, with brain exhibiting most differentially expressed genes, emphasizing its unique sensitivity fluctuations. Pathway analyses revealed expected enrichment ion transport osmoregulation pathways across all tissues. Furthermore, tissue-specific associated stress, reproduction, growth, immune responses, methylation, neurological development identified context salinization. Rigorous validation RNA-seq data through quantitative PCR (qPCR) underscored robustness consistency our findings platforms. investigation unveils intricate mechanisms steering response minnows confronting diverse stressors. comprehensive analysis sheds light on underlying genetic governing salinity-stressed environments, offering essential knowledge for conservation management ecosystems facing

Language: Английский

Citations

5
Proposal of novel Predicted No Effect Concentrations (PNEC) for metals in freshwater using Species Sensitivity Distribution for different taxonomic groups DOI Creative Commons
Gisele Alves Miranda, Fábio Soares dos Santos, M. Cardoso

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 10, 2025

Water pollution by metals and metalloids promotes toxic effects to aquatic biota especially in mining regions. Environmental legislation applied protect life from the toxicity of relies on definition protective values (PVs) for each compound. Among methods used define PVs, Species Sensitivity Distribution (SSD) curves enable derivation Predicted No Effect concentration (PNEC). In this context, is one first studies propose construction acute chronic split SSD built separately three groups freshwater organisms (algae, invertebrates fish) derive PNEC 14 most commonly observed iron ore tailings. Data construct were derived USEPA ECOTOX knowledgebase EnviroTox databases segregated according organism group as "acute" or "chronic" toxicity. Then, using a minimum nine species determine hazardous 5% (HC5) group. Once derived, framework was proposed calculate Bioavailabity Factor (BioF) adjust local bioavailability conditions considering water quality characteristics different The lowest algae corresponded Silver (Ag). Nearly half calculated below current PVs practice Brazil, United States (US), Kingdom (UK), Canada European Union (EU). Results reinforce pertinence of: (i) splitting metals; (ii) taking into consideration correct conditions. addition, outcomes suggest that it critical rethink related protection, mainly Brazil Minas Gerais state, region known extensive activity. Finally, obtained study may be ecological risk assessment studies, areas affected other activities result metalloids, such Brazil.

Language: Английский

Citations

0
Heatwaves, elevated temperature, and insecticide-induced effects at different trophic levels of a freshwater ecosystem DOI Creative Commons
Markus Hermann, Lara M. Schuijt, Dania Albini

et al.

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121566 - 121566

Published: April 1, 2025

Language: Английский

Citations

0
Beyond Dosage: The Need for More Realistic Research Scenarios to Understand Pesticide Impacts on Agricultural Soils DOI Creative Commons
Judith Riedo, Matthias C. Rillig, Florian Walder

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Pesticides play a crucial role in modern agriculture, yet they pose considerable risks to soil health and ecosystem integrity. Current risk assessment research often relies on simplified models, focusing single substances under standardized conditions failing reflect realistic exposure scenarios. We call for paradigm shift toward incorporating agroecological that evaluates pesticide effects more complex dynamic conditions, including mixtures, application frequency, diverse properties, interactions with other environmental stressors. Additionally, multiseasonal long-term persistence of pesticides soils must be considered. By integrating these multidimensional factors, such experimental can yield valuable insights improve frameworks, ensuring accurately represent the complexity agricultural systems support sustainable management practices.

Language: Английский

Citations

0
Environmental effects monitoring of offshore oil and gas activities on the Norwegian continental shelf: A review DOI Creative Commons
Jonny Beyer, Kari E. Ellingsen, Nigel G. Yoccoz

et al.

Marine Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 107166 - 107166

Published: April 1, 2025

Language: Английский

Citations

0
To Split or Not to Split: Characterizing Chemical Pollution Impacts in Aquatic Ecosystems with Species Sensitivity Distributions for Specific Taxonomic Groups DOI Creative Commons
Susan Anyango Oginah, Leo Posthuma, Michael Zwicky Hauschild

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(39), P. 14526 - 14538

Published: Sept. 21, 2023

Bridging applied ecology and ecotoxicology is key to protect ecosystems. These disciplines show a mismatch, especially when evaluating pressures. Contrasting ecology, ecotoxicological impacts are often characterized for whole species assemblages based on Species Sensitivity Distributions (SSDs). SSDs statistical models describing per chemical across-species sensitivity variation laboratory toxicity tests. To assist in the aligning of improve decision-support uses SSDs, we investigate taxonomic-group-specific algae/cyanobacteria/aquatic plants, invertebrates, vertebrates 180 chemicals with sufficient test data. We that splitting improves pollution impact assessments specific mode action and, surprisingly, narcotic chemicals. provide framework can be serve environmental protection, life cycle assessment, management freshwater illustrate using split has potentially large implications SSD-based outputs around globe.

Language: Английский

Citations

10