The Allium cepa comet assay for environmental sample assessment: a scoping review DOI
Carlotta Alias, Ilaria Zerbini,

Claudia Zani

et al.

Mutagenesis, Journal Year: 2024, Volume and Issue: 39(4-5), P. 219 - 237

Published: July 1, 2024

Abstract The purposes of this review were to investigate the application comet assay in Allium cepa root cells assess genotoxicity environmental samples and analyse experimental procedures employed. A literature search was performed selecting articles published between January 2000 October 2023 from online databases using combined terms ‘comet assay’ ‘A. cepa’. Only 18 papers met inclusion criteria. None these first eight years (2000–2007), highlighting increasing interest on A. over last decade. majority selected studies (15/18, 83%) belonging water compartment onion bulbs. Half (9/18) conducted demonstrate DNA damaging effect sample, while other half not only recognized presence genotoxic agents but also addressed possible remediation measures. Detailed analysis revealed heterogeneity many key steps, such as exposure time, test controls, nuclei isolation solutions, duration electrophoresis, number scored. This has shown that cepa, although an appropriate tool, is underutilized toxicology. Greater standardization could lead its more widespread use, providing valuable information ability different processes mitigate their negative effects plants.

Language: Английский

Evaluation of the toxicity of pervious concretes with WFS through germination tests with Eruca sativa (arugula) and Triticum aestivum (wheat) seeds DOI Creative Commons
Luciene Gachet Ferrari Domingues, Edison Luis Candian Filho, Bruna de Jesus Moreira

et al.

Matéria (Rio de Janeiro), Journal Year: 2025, Volume and Issue: 30

Published: Jan. 1, 2025

ABSTRACT Concrete is the most used construction material, which results in harmful impacts on environment due to consumption of natural resources. Hence need use alternative materials, e.g., waste from sector and even other production sectors. This context includes development concrete with sustainable functionality, such as pervious incorporation foundry sand (WFS), a generated by sector. However, there scientific gap focusing environmental viability concretes. In this sense, study aims evaluate toxicity pevious concretes WFS, through germination tests Eruca sativa (arugula) Triticum aestivum (wheat) seeds. The statistical analysis showed that was no significant effect WFS rate for both seeds used. Regarding root growth, it observed II (>% Portland cement) had lower impact arugula (more sensitive). Therefore, developed found be safe relation phytotoxicity.

Language: Английский

Citations

0
Toxicological and genotoxicological assessment of water extracts of sewage sludge and other biogenic wastes: A piece of the SLURP jigsaw puzzle DOI Creative Commons
Carlotta Alias, Donatella Feretti, Ilaria Zerbini

et al.

Chemosphere, Journal Year: 2025, Volume and Issue: 374, P. 144175 - 144175

Published: Feb. 20, 2025

Given the considerable quantities of biogenic matrices employed in agricultural applications, there is growing concern about potential negative effects resulting from presence harmful contaminants. The project "SLURP - SLUdge Recovery Agriculture: Environment and Health Protection" planned a multi-stage approach which application wide battery bioassays was proposed as an effective tool to measure direct interaction with different components ecosystem, molecular whole organism level. aim present study, part "SLURP" project, characterise toxicological genotoxicological properties water extracts wastes using several assays based on plant, bacterial human cells. aqueous four sewage sludges, liming material, two manure slurries swine bovine origin, digestate compost were chemically characterised for inorganic ions heavy metals. Then analysed tests A.cepa, C.sativus, L.sativum, S.typhimurium hepatoma cell line (HepG2) assess toxicity (seed germination, root elongation, proliferation), mutagenicity genotoxicity (primary DNA damage, chromosomal aberrations). exhibited chemical heterogeneity. Ammonia nitrogen, Ca2+, Fe Zn most abundant elements. Toxic caused A.cepa L.sativum by all extracts, while non-toxic Genotoxic instead almost at least highest dose tested, only samples one sludge, digestate, compost, damage None induced mutagenic S.typhimurium. A comprehensive interpretation these results can be achieved through integrated evaluation eco-toxicological data obtained throughout entire project.

Language: Английский

Citations

0
To spread or not to spread? Assessing the suitability of sewage sludge and other biogenic wastes for agriculture reuse DOI
Giorgio Bertanza, Alessandro Abbà, Carlotta Alias

et al.

MethodsX, Journal Year: 2024, Volume and Issue: 12, P. 102599 - 102599

Published: Feb. 7, 2024

Language: Английский

Citations

3
The Allium cepa comet assay for environmental sample assessment: a scoping review DOI
Carlotta Alias, Ilaria Zerbini,

Claudia Zani

et al.

Mutagenesis, Journal Year: 2024, Volume and Issue: 39(4-5), P. 219 - 237

Published: July 1, 2024

Abstract The purposes of this review were to investigate the application comet assay in Allium cepa root cells assess genotoxicity environmental samples and analyse experimental procedures employed. A literature search was performed selecting articles published between January 2000 October 2023 from online databases using combined terms ‘comet assay’ ‘A. cepa’. Only 18 papers met inclusion criteria. None these first eight years (2000–2007), highlighting increasing interest on A. over last decade. majority selected studies (15/18, 83%) belonging water compartment onion bulbs. Half (9/18) conducted demonstrate DNA damaging effect sample, while other half not only recognized presence genotoxic agents but also addressed possible remediation measures. Detailed analysis revealed heterogeneity many key steps, such as exposure time, test controls, nuclei isolation solutions, duration electrophoresis, number scored. This has shown that cepa, although an appropriate tool, is underutilized toxicology. Greater standardization could lead its more widespread use, providing valuable information ability different processes mitigate their negative effects plants.

Language: Английский

Citations

3