Anti-Obesity Potential of Secamone afzelii K. Leaf and Vine Ethanol Extract: Insights into Lipase Inhibition and Secondary Metabolites DOI Creative Commons
Franklyn Nonso Iheagwam,

Gloria Aniedi Itina,

Oyinkansola Ayobolanle Faluyi

et al.

Sci, Journal Year: 2024, Volume and Issue: 6(4), P. 70 - 70

Published: Nov. 1, 2024

Pancreatic lipase is an enzyme crucial for breaking down fats through hydrolysis, and inhibiting it important managing obesity. This study evaluated the inhibitory profile of leaf (SALE) vine (SAVE) Secamone afzelii, explored modes inhibition, identified primary compounds responsible this effect, examined their molecular interactions with using in vitro silico techniques. SALE (IC50: 0.41 ± 0.02 mg/mL) exhibited higher activity compared to SAVE 0.95 0.05 mg/mL), although was significantly lower than orlistat 0.07 0.00 across all concentrations. S. afzelii extracts inhibited uncompetitive mode inhibition. Gas chromatography-mas spectroscopy 54 47 SAVE, respectively, 9,12-octadecadienoic acid (Z, Z)-, n-hexadecanoic 4S,6R-dimethyl-7R-hydroxynonan-3-one as most abundant both extracts. The binding energy top five ligands from ranged −7.7 −6.6 kcal/mol, outperforming that (−4.4 kcal/mol). had similar poses stabilised by hydrogen π CYS299, SER301, CYS304, ASN425 VAL426. These were predicted possess promising pharmacokinetic, lipophilic hydrophilic properties. results offer insights into traditional use treating obesity valuable information on potential drug candidates can be optimised combating disease.

Language: Английский

Anti-Obesity Potential of Secamone afzelii K. Leaf and Vine Ethanol Extract: Insights into Lipase Inhibition and Secondary Metabolites DOI Creative Commons
Franklyn Nonso Iheagwam,

Gloria Aniedi Itina,

Oyinkansola Ayobolanle Faluyi

et al.

Sci, Journal Year: 2024, Volume and Issue: 6(4), P. 70 - 70

Published: Nov. 1, 2024

Pancreatic lipase is an enzyme crucial for breaking down fats through hydrolysis, and inhibiting it important managing obesity. This study evaluated the inhibitory profile of leaf (SALE) vine (SAVE) Secamone afzelii, explored modes inhibition, identified primary compounds responsible this effect, examined their molecular interactions with using in vitro silico techniques. SALE (IC50: 0.41 ± 0.02 mg/mL) exhibited higher activity compared to SAVE 0.95 0.05 mg/mL), although was significantly lower than orlistat 0.07 0.00 across all concentrations. S. afzelii extracts inhibited uncompetitive mode inhibition. Gas chromatography-mas spectroscopy 54 47 SAVE, respectively, 9,12-octadecadienoic acid (Z, Z)-, n-hexadecanoic 4S,6R-dimethyl-7R-hydroxynonan-3-one as most abundant both extracts. The binding energy top five ligands from ranged −7.7 −6.6 kcal/mol, outperforming that (−4.4 kcal/mol). had similar poses stabilised by hydrogen π CYS299, SER301, CYS304, ASN425 VAL426. These were predicted possess promising pharmacokinetic, lipophilic hydrophilic properties. results offer insights into traditional use treating obesity valuable information on potential drug candidates can be optimised combating disease.

Language: Английский

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