Mangiferin (mango) attenuates AOM-induced colorectal cancer in rat’s colon by augmentation of apoptotic proteins and antioxidant mechanisms

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 8, 2024

Abstract Mangiferin (MF) is a natural C-glucosylxantone compound that has many substantial curative potentials against numerous illnesses including cancers. The present study's goal to appraise the chemo preventive possessions of MF on azoxymethane (AOM)-mediated colonic aberrant crypt foci (ACF) in rats. Rats clustered into 5 groups, negative control (A), inoculated subcutaneously with normal saline twice and nourished 0.5% CMC; groups B-E injected 15 mg/kg followed by ingestion CMC (B, cancer control); intraperitoneal inoculation 35 5-fluorouracil (C, reference rats) or 30 (D) 60 (E) MF. Results gross morphology colorectal specimens showed significantly lower total ACF incidence MF-treated rats than controls. colon tissue examination increased availability bizarrely elongated nuclei, stratified cells, higher depletion submucosal glands compared treatment caused regulation pro-apoptotic (increased Bax) proteins reduced β-catenin) expression. Moreover, fed had glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) concentrations their homogenates. supplementation down-shifted pro-inflammatory cytokines (transforming growth factor-α interleukine-6) up-shifted anti-inflammatory (interleukine-10) based serum analysis. chemo-protective mechanistic AOM-induced ACF, shown values penetration, could be correlated its positive modulation apoptotic cascade, antioxidant enzymes, inflammatory originating from AOM oxidative stress insults.

Language: Английский

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Mechanisms of anti-ulcer actions of Prangos pabularia (L.) in ethanol-induced gastric ulcer in rats

Saudi Pharmaceutical Journal, Journal Year: 2023, Volume and Issue: 31(12), P. 101850 - 101850

Published: Oct. 29, 2023

Peptic ulcer disease is the greatest digestive disorder that has increased incidence and recurrence rates across all nations. Prangos pabularia (L.) been well documented as a folkloric medicinal herb utilized for multiple conditions including gastric ulcers. Hence, target study was investigation gastro-protection effects of root extracts (REPP) on ethanol-mediated stomach injury in rats. Sprague Dawley rats were clustered 5 cages: A B, normal control pre-ingested with 1 % carboxymethyl cellulose (CMC)); C, reference had 20 mg/kg omeprazole; D E, pre-supplemented 250 500 REPP, respectively. After one hour, group given orally CMC, groups B-E 100 ethanol. The area, acidity, wall mucus stomachs determined. tissue homogenates examined antioxidant MDA contents. Moreover, tissues analyzed by histopathological immunohistochemically assays. Acute toxicity results showed lack any toxic or histological changes exposed to 2 g/kg REPP ingestion. controls extensive mucosal damage lower juice reduced pH. treatment caused significant reduction ethanol-induced lacerations represented an upsurge glycoproteins (increased PAS), decrease leukocyte infiltration, positively modulated Bax HSP 70 proteins, consequently lowered areas. supplementation oxidative stress SOD, CAT, PGE2, MDA) inflammatory cytokines (decreased serum TNF-α, IL-6, IL-10) levels. outcomes could be scientific evidence back-up use A. Judaica remedy stress-related disorders (gastric ulcer).

Language: Английский

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Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines

Saudi Journal of Biological Sciences, Journal Year: 2023, Volume and Issue: 30(6), P. 103678 - 103678

Published: May 8, 2023

In an increasing interest in natural antiulcer compounds that may have gastric healing effects and possibly prevent ulcer recurrence, Polygonatum odoratum appears as a strong candidate. The gastroprotective potentials of P. rhizome extract (PORE) were explored on ethanol-induced ulceration rats. Sprague Dawley rats caged 5 groups, normal control received CMC (1% carboxymethyl cellulose). Omeprazole (20 mg/kg) was given to reference Rats. Experimental treated with 250 mg/kg 500 PORE, respectively. After hour, the 1% CMC, whereas rat groups 2-5 absolute ethanol by oral gavage. 60 min, anesthesia sacrificed. Dissected tissue analyzed histopathological immunohistochemical techniques. PORE treatment significantly lowered injury, shown up-surging pH mucus content, reduced leukocyte infiltration, lower ulcerative areas mucosal layers, increased antioxidants (SOD CAT) (MDA) levels. Furthermore, pre-treated showed expression Periodic acid-Schiff (PAS), HSP-70 protein, decreased Bax protein their epithelial layers. important regulation inflammatory cytokines decreasing TNF-a, IL-6 IL-10 values. detected biological activity is encouraging presents scientific evidence for its traditional use gastroprotection agent however further studies are required determine exact phytochemicals mechanism pathway responsible this bioactivity.

Language: Английский

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A Traditional Gum Exudate From Pistacia atlantica Ameliorates Etha‐Nol‐Mediated Gastric Ulcer in Rats: Possible Molecular Mechanisms

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(3)

Published: March 1, 2025

Pistacia atlantica (Bene) is a native Mediterranean plant that exudates resinous therapeutic oleoresin gum used for many inflammatory-related diseases. Therefore, the present study evaluates acute toxicity and gastroprotective effects of (PAG) on ethanol-mediated gastric ulcers in rats. Sprague Dawley rats (30) were placed 5 cages: Group A received 10% tween 20; B, ulcer control, reference (C, 20 mg/kg omeprazole), groups D E 250 500 PAG, respectively. After 60 min, Groups B-E absolute ethanol (5 mL/kg). The results showed lack any physiological alterations supplemented with up to g/kg PAG. In trial, controls exhibited extensive mucosal injuries, reduced stomach mucus secretion, highly acidic stomach, increased lesion areas. Ethanol ingestion caused significant inflammatory cell infiltration oxidative stress indicators their tissues. revealed HSP 70, elevated Bax protein expressions, lowered antioxidant enzymes up-regulated MDA contents. PAG treatment restored these negative ethanol, which could be because its terpenoids, phenolics, flavonoids potentials positively modulated defense barriers (mucopolysaccharides), antioxidants, significantly pH cytokines (TNF-α IL-6). gastro-prophylactic are validated by modulatory actions mechanisms, providing scientific evidence future biochemical characterizations.

Language: Английский

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Gastroprophylactic Effects of p-Cymene in Ethanol-Induced Gastric Ulcer in Rats

Processes, Journal Year: 2022, Volume and Issue: 10(7), P. 1314 - 1314

Published: July 4, 2022

The prevalence of gastric ulcers has increased in recent years, mainly because non-steroidal anti-inflammatory drug utilization. Therefore, the current study investigates gastroprotective effect p-Cymene on absolute ethanol-induced acute mucosal hemorrhagic lesions rats. Thirty Sprague Dawley rats were randomly separated into five groups: normal control, ulcer reference, and two experimental groups. control groups orally fed with 0.5% carboxymethylcellulose (CMC). reference group was 20 mg/kg omeprazole. 30 60 p-Cymene, respectively. After one hour, CMC, 2–5 given alcohol. another hour all sacrificed. showed severe superficial decreased mucus secretion pH content. significantly reduced lesions, as evidenced by content, area, or absence edema, leucocyte infiltration subcutaneous layer. In homogenate, displayed a significant increase superoxide dismutase (SOD), catalase (CAT) activities, prostaglandin E2 (PGE2), malondialdehyde (MDA) level. addition, intensity periodic acid–Schiff (PAS) stain epithelium, produced up-regulation HSP 70 protein down-regulation Bax stomach well reduction levels tumor necrotic factor-alpha interleukin-6, while level interleukin-10 increased. TNF-a IL-6, IL-10. Acute toxicity higher dose 500 did not manifest any toxicological signs could enhance defensive mechanisms against lesions. effects that be attributed to its antioxidant nature, ability secretion, endogenous enzymes (SOD, CAT, PGE2), reduce MDA level, up-regulate protein, down-regulate modulate inflammatory cytokines.

Language: Английский

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Ethnobotanical, Phytochemistry, and Pharmacological Activity of Onosma (Boraginaceae): An Updated Review

Molecules, Journal Year: 2022, Volume and Issue: 27(24), P. 8687 - 8687

Published: Dec. 8, 2022

The genus Onosma belongs to the Boraginaceae family and contains over 230 species. present review sheds light on ethnopharmacology, phytoconstituents, bioactivity, toxicology of species from previous investigations. Furthermore, paper also highlights unresolved issues for future included studies available Google Scholar Baidu Scholar, Science Direct, SciFinder, Wiley Online Library, Web Science. Until now, more than 200 chemical compounds have been detected Onosma, including naphthoquinone (33), flavonoids (30), hydrocarbon (23), phenolic (22), ester (17), alkaloids (20), aromatics (12), carboxylic acid (11), fatty acids (9), terpenoids (10), while most important ones are rosmarinic, ferulic, protocatechuic, chlorogenic, caffeic, p-coumaric acids, apigenin. reported as traditional medicine wound healing, heart disease, kidney disorders, pharmacological investigations revealed that extracts phytochemicals different therapeutic properties antioxidant, enzyme inhibitory, antitumor, hepatoprotective, antiviral, anti-inflammatory, antimicrobial actions. summarized knowledge in this provides valuable ideas current drug discovery a motivation further investigation Onosma.

Language: Английский

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GC-MS Analysis of Bioactive Compounds in Methanolic Extracts of Papaver decaisnei and Determination of Its Antioxidants and Anticancer Activities

Journal of Food Quality, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 12

Published: April 23, 2022

The Papaver L. plant (Papaver decaisnei) has ethnobotanical records in many countries including Iraqi Kurdistan. current study investigates the methanol (99.9%) extracts (10 μg/mL) of roots, leaves, and flowers decaisnei terms phytochemistry by gas chromatography-mass spectrophotometry GC-MS, vitro antioxidant activity radical scavenging reducing power assays, finally, anticancer actions as IC50 (inhibitory concentration at 50%) against human colorectal adenocarcinoma (Caco-2), mammary cancer cells (MCF-7), cervical carcinoma (HeLa) cells. results showed 22, 19, 17 chemicals for P. decaisnei, respectively. prevalent organic compounds were alkaloids (62.03%), phenolics (55.43%), fatty acids (42.51%), esters (32.08%), terpenoids (25.59%), phytosterols (15.68%), namely, roemerine (70.44%), 9,12,15-octadecatrien-1-ol (37.45%), hexadecanoic acid (33.72%), decarbomethoxytabersonine (24.49%), γ-sitosterol (11.22%). organs was within 39.1–143.5 μg/mL DPPH, 135.4–276.4 ABTS, 12.4–34.3 FRAP, 42.6–75.8 CUPRAC assays. found 125.3–388.4 all tested cell lines (Caco-2, MCF-7, HeLa). detected bioactivity encourage future isolation those remarkable (reomerine) potential usage pharmaceutical industry.

Language: Английский

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Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

Current Issues in Molecular Biology, Journal Year: 2023, Volume and Issue: 45(2), P. 885 - 902

Published: Jan. 18, 2023

Onosma species (Boraginaceae) are well known as medicinal plants due to their wide range of pharmaceutical potential. The present study aims investigate the anticancer (in vitro) and chemo-protective vivo) efficacies mutabilis extract (OME) in azoxymethane (AOM)-induced aberrant crypt foci (ACF) rats. vitro antiproliferative effects OME were determined on two human tumor cell lines (Caco-2 HT-29) via MTT assay. vivo chemoprotective investigated by performing various biochemical analyses serum tissue homogenates albino rats, along with determining oxidative stress biomarkers. Inflammatory biomarkers colon, colonic gross morphology (by methylene blue), ACF formation, histopathology (H & E stain) determined. immunohistochemistry tissues was also assessed Bax Bcl-2 protein expression. results showed that antitumor activity against Caco-2 HT-29 colorectal cancer cells ranged between 22.28-36.55 µg/mL. supplementation caused a significant drop values improved rats shown up-regulation down-regulation expressions. These outcomes reveal O. may have efficiency AOM-induced colon represented attenuation formation possibly through inhibition free radicals, inflammation, stimulation antioxidant armory (SOD, CAT, GPx) positive regulation Nrf2-Keap1 pathway.

Language: Английский

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Onosma mutabilis: Phytochemical composition, antioxidant, cytotoxicity, and acute oral toxicity

Food Science & Nutrition, Journal Year: 2021, Volume and Issue: 9(10), P. 5755 - 5764

Published: Aug. 28, 2021

Abstract The traditional use of Onosma L. species as a remedy motivated scientists to discover great biological/pharmacological potentials in this plant. In the current study, addition phytochemical composition methanol (MeOH), water, and ethyl acetate extract aerial parts mutabilis Boiss., an endemic plant flora Kurdistan, Iraq, vitro antioxidant, cytotoxicity, oral toxicity activity were investigated. Results total phenolic flavonoid tests show MeOH superiority, results Gas chromatography–mass spectrophotometer(GS/GS‐MS) 18 chemical compounds extract, majority detected alkaloids (78.77%) steroids (11.48%), namely 5,8‐dihydroxy‐2‐(4‐methylpent‐3‐enyl) naphthalene‐1,4‐dione (48.60%), 3‐O‐Methyl‐d‐glucose (27.49%), β‐Sitosterol (6.81%), Phenol, 2,4‐bis (1,1‐dimethyl ethyl)‐, phosphite (3.46%), 24,25‐Dihydroxycholecalciferol (3.14%). antioxidant superiority phosphomolybdenum assay, radical scavenging [on 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS)] assays, reducing power [cupric capacity (CUPRAC) ferric (FRAP)] assays (1.45, 3.54, 2.33, 1.12, 1.62, mg/ml, respectively). cytotoxicity are presented IC 50 (inhibitory concentration at 50%) on prostate cancer cells (DU‐145), mammary (MCF‐7), human cervix carcinoma (Hep2c), which values ranged from 28.79 41.83 μg/ml. acute dose‐dependent trail (100, 200, 300, 600 mg/kg MeOH) absence behavior appearance changes female Wister rats. Overall, O. exhibited significant natural probably because its polar phytochemicals, could be alternative source for remedial, nutrient, cosmetic manufacture.

Language: Английский

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Gastroprotective, Biochemical and Acute Toxicity Effects of Papaver decaisnei against Ethanol-Induced Gastric Ulcers in Rats

Processes, Journal Year: 2022, Volume and Issue: 10(10), P. 1985 - 1985

Published: Oct. 1, 2022

Papaver decaisnei (P. decaisnei) has been used as folkloric medicine for many health issues including gastric problems. The current study investigates the gastroprotective roles of P. against ethanol-induced ulcers in rodents. Sprague Dawley rats (30) were separated into five groups: normal group (G1) and ulcer control (G2) orally administered 0.5% carboxymethylcellulose (CMC); reference (G3) was 20 mg/kg Omeprazole; two experimental groups fed with 200 (G4) 400 (G5) extract (PDE), respectively. Next, given absolute ethanol sacrificed analysis mucosal injury through microscopic, enzymatic, histologic, immunohistochemistry assays. controls showed significant superficial hemorrhagic lesions, a decreased wall mucus edema production, whereas fewer found plant-treated rats. Furthermore, PDE pre-treated had significantly reduced periodic acid-Schiff (PAS) staining intensity, produced upregulation HSP70 protein, downregulation Bax protein expressions stomach epithelium. displayed role endogenous antioxidant enzymes (SOD, CAT, PGE2), malondialdehyde (MDA), TNF-a, IL-6, upraised IL-10 levels. Based on positive impacts, can be proposed protective/treatment agent lesions.

Language: Английский

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