Antioxidant and In Vitro Hepatoprotective Activities of a Polyphenol-Rich Fraction from the Peel of Citrus lumia Risso (Rutaceae) DOI Creative Commons
Vincenzo Musolino, Antonio Cardamone, Rosario Mare

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(8), P. 1209 - 1209

Published: April 15, 2025

Citrus lumia Risso is an ancient, cultivated Mediterranean lime belonging to the Rutaceae family. It a species extremely difficult retrieve, but it still found in some private gardens certain regions of Southern Italy. fruits are rich source bioactive compounds, particularly polyphenols, which have been linked reduction risk several metabolic diseases. Here, hesperidium peel extracts were obtained by maceration with ethanol:water mixtures different proportions (50:50, 80:20, 0:100) and resulting crude then passed through glass column containing adsorbent resins concentrate polyphenolic compounds. After phytochemical characterization, evaluated for antioxidant activity using electron paramagnetic resonance (EPR) spectroscopy. Finally, water polyphenolic-rich extract (ClumWp), was highest flavonoid content (18.355 ± 1.607 mg/mL) strongest against hydroxyl radical, tested evaluate its potential protective effects on lipid accumulation both 2D hepatocyte cultures 3D spheroids. Treatment 25 50 μg/mL resulted intracellular HepG2 liver cell line, while treatment 100 µg/mL ClumWp + LX2 In addition, significantly increased ATP levels spheroids compared those untreated, suggesting ability restore promote production. Our results highlight that study neglected species, such as Risso, remains valuable opportunity valorize biodiversity, especially context applications improve human health. particular, fraction showed promising metabolism cellular energy balance may prove disorders MASLD, where disrupts normal functions.

Language: Английский

Antioxidant and In Vitro Hepatoprotective Activities of a Polyphenol-Rich Fraction from the Peel of Citrus lumia Risso (Rutaceae) DOI Creative Commons
Vincenzo Musolino, Antonio Cardamone, Rosario Mare

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(8), P. 1209 - 1209

Published: April 15, 2025

Citrus lumia Risso is an ancient, cultivated Mediterranean lime belonging to the Rutaceae family. It a species extremely difficult retrieve, but it still found in some private gardens certain regions of Southern Italy. fruits are rich source bioactive compounds, particularly polyphenols, which have been linked reduction risk several metabolic diseases. Here, hesperidium peel extracts were obtained by maceration with ethanol:water mixtures different proportions (50:50, 80:20, 0:100) and resulting crude then passed through glass column containing adsorbent resins concentrate polyphenolic compounds. After phytochemical characterization, evaluated for antioxidant activity using electron paramagnetic resonance (EPR) spectroscopy. Finally, water polyphenolic-rich extract (ClumWp), was highest flavonoid content (18.355 ± 1.607 mg/mL) strongest against hydroxyl radical, tested evaluate its potential protective effects on lipid accumulation both 2D hepatocyte cultures 3D spheroids. Treatment 25 50 μg/mL resulted intracellular HepG2 liver cell line, while treatment 100 µg/mL ClumWp + LX2 In addition, significantly increased ATP levels spheroids compared those untreated, suggesting ability restore promote production. Our results highlight that study neglected species, such as Risso, remains valuable opportunity valorize biodiversity, especially context applications improve human health. particular, fraction showed promising metabolism cellular energy balance may prove disorders MASLD, where disrupts normal functions.

Language: Английский

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