Exploring the Therapeutic Promise of Drynaria Coronans: Phytochemical Analysis, Antioxidant Capacity, α-Amylase Inhibition with Safety Assessment DOI Creative Commons
Prabhat Kumar Jha, Sindhu KC, Ram Kishor Yadav

et al.

Published: Feb. 10, 2025

Article Exploring the Therapeutic Promise of Drynaria Coronans: Phytochemical Analysis, Antioxidant Capacity, α-Amylase Inhibition with Safety Assessment Prabhat Kumar Jha 1,2,*, Sindhu KC 1, Ram Kishor Yadav Bipindra Pandey 2, Sandesh Paudel Ravindra Khadka Kushal Subedi Sushil Panta 1,* 1 School Health and Allied Sciences, Pokhara University, Kaski 33700, Nepal 2 Department Pharmacy, Madan Bhandari Academy Hetauda 44100, * Correspondence: [email protected] (P.K.J.); [email protected] (S.P.); Tel.: +977-9864512660 +977-9846032882 (S.P.) Received: December 2024; Revised: 9 January 2025; Accepted: 7 February Published: 10 2025 Abstract: Background: Secondary metabolites derived from plants have been identified as potential natural antioxidants, exhibiting robust capabilities in neutralizing oxidative stress that can emerge under various pathological conditions. Aim: The aim this study was to analyze phytochemical composition ethanolic extract coronans (D. coronans), its antioxidant along α-amylase inhibition oral toxicity studies. Methods: Preliminary qualitative screening conducted for D. while quantitative analysis involved estimation total phenolic flavonoid contents. In vitro activities were evaluated by using ferrous reducing-antioxidant power (FRAP), hydrogen peroxide (H2O2) scavenging activity, nitric oxide activity DPPH free radical assays. antidiabetic assay. vivo acute studies rats per Organization Economic Cooperation Development guidelines. Results: extraction yield found be 11.94% ethanol primary solvent plant sample a 4:1 ratio. rhizome revealed presence secondary metabolites, including alkaloids, phenols, tannins, glycosides, carbohydrates, which further validated TLC profiling. dried contained 56.38 ± 0.09 mg gallic acid equivalent (GAE/g) phenols 202.54 0.22 quercetin equivalents (QE/g) flavonoids. demonstrated notable properties, IC50 values 43.59 (DPPH), 758.94 (NO), 715.60 (H2O2), also strong reducing (0.164 0.011) at 700 nm. Additionally, it showed effective an 889.84 μg/mL. When tested dose 5000 mg/kg, no behavioral changes, morbidity, or mortality observed Swiss albino rats. Conclusion: rhizomes positive correlation contents antioxidant, inhibitory α-amylase.

Language: Английский

Exploring the Therapeutic Promise of Drynaria Coronans: Phytochemical Analysis, Antioxidant Capacity, α-Amylase Inhibition with Safety Assessment DOI Creative Commons
Prabhat Kumar Jha, Sindhu KC, Ram Kishor Yadav

et al.

Published: Feb. 10, 2025

Article Exploring the Therapeutic Promise of Drynaria Coronans: Phytochemical Analysis, Antioxidant Capacity, α-Amylase Inhibition with Safety Assessment Prabhat Kumar Jha 1,2,*, Sindhu KC 1, Ram Kishor Yadav Bipindra Pandey 2, Sandesh Paudel Ravindra Khadka Kushal Subedi Sushil Panta 1,* 1 School Health and Allied Sciences, Pokhara University, Kaski 33700, Nepal 2 Department Pharmacy, Madan Bhandari Academy Hetauda 44100, * Correspondence: [email protected] (P.K.J.); [email protected] (S.P.); Tel.: +977-9864512660 +977-9846032882 (S.P.) Received: December 2024; Revised: 9 January 2025; Accepted: 7 February Published: 10 2025 Abstract: Background: Secondary metabolites derived from plants have been identified as potential natural antioxidants, exhibiting robust capabilities in neutralizing oxidative stress that can emerge under various pathological conditions. Aim: The aim this study was to analyze phytochemical composition ethanolic extract coronans (D. coronans), its antioxidant along α-amylase inhibition oral toxicity studies. Methods: Preliminary qualitative screening conducted for D. while quantitative analysis involved estimation total phenolic flavonoid contents. In vitro activities were evaluated by using ferrous reducing-antioxidant power (FRAP), hydrogen peroxide (H2O2) scavenging activity, nitric oxide activity DPPH free radical assays. antidiabetic assay. vivo acute studies rats per Organization Economic Cooperation Development guidelines. Results: extraction yield found be 11.94% ethanol primary solvent plant sample a 4:1 ratio. rhizome revealed presence secondary metabolites, including alkaloids, phenols, tannins, glycosides, carbohydrates, which further validated TLC profiling. dried contained 56.38 ± 0.09 mg gallic acid equivalent (GAE/g) phenols 202.54 0.22 quercetin equivalents (QE/g) flavonoids. demonstrated notable properties, IC50 values 43.59 (DPPH), 758.94 (NO), 715.60 (H2O2), also strong reducing (0.164 0.011) at 700 nm. Additionally, it showed effective an 889.84 μg/mL. When tested dose 5000 mg/kg, no behavioral changes, morbidity, or mortality observed Swiss albino rats. Conclusion: rhizomes positive correlation contents antioxidant, inhibitory α-amylase.

Language: Английский

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