In vivo autofluorescence lifetime imaging of the Drosophila brain captures metabolic shifts associated with memory formation DOI Open Access

Philémon Roussel,

Mingyi Zhou, Chiara Stringari

et al.

Published: March 31, 2025

Neuronal energy regulation is increasingly recognized as a critical factor underlying brain functions and their pathological alterations, yet the metabolic dynamics that accompany cognitive processes remain poorly understood. As label-free minimally invasive technique, fluorescence lifetime imaging (FLIM) of coenzymes NADH NADPH (collectively referred to NAD(P)H) offers possibility resolve cellular profiles with high spatial precision. However, NAD(P)H FLIM’s capacity detect subtle changes in neuronal metabolism associated cognition has not been demonstrated. In this study, we applied FLIM map Drosophila neurons vivo across multiple scales, focusing on primary centers for associative memory: mushroom bodies (MBs). At broad scale, obtained an overview signatures main tissue identified marked difference between neuropil cortex areas. finer our findings revealed notable heterogeneity basal distinct MB neuron subtypes. Measurements performed after olfactory learning also uncovered subtype-specific shift memory formation, demonstrating utility detecting physiology-driven linked function. These results establish promising framework studying cerebral vivo.

Language: Английский

Glia as Functional Barriers and Signaling Intermediaries DOI
Vilaiwan M. Fernandes, Vanessa J. Auld, Christian Klämbt

et al.

Cold Spring Harbor Perspectives in Biology, Journal Year: 2024, Volume and Issue: 16(1), P. a041423 - a041423

Published: Jan. 1, 2024

Vilaiwan M. Fernandes1, Vanessa Auld2 and Christian Klämbt3 1Department of Cell Developmental Biology, University College London, London UC1E 6DE, United Kingdom 2Department Zoology, British Columbia, Vancouver, Columbia V6T 1Z4, Canada 3Institute for Neuro- Behavioral Münster, Münster 48149, Germany Correspondence: klaembt{at}uni-muenster.de

Language: Английский

Citations

14
Experience-dependent glial pruning of synaptic glomeruli during the critical period DOI Creative Commons

Nichalas Nelson,

Dominic J. Vita, Kendal Broadie

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 20, 2024

Abstract Critical periods are temporally-restricted, early-life windows when sensory experience remodels synaptic connectivity to optimize environmental input. In the Drosophila juvenile brain, critical period drives synapse elimination, which is transiently reversible. Within olfactory neuron (OSN) classes synapsing onto single projection neurons extending brain learning/memory centers, we find glia mediate experience-dependent pruning of OSN glomeruli downstream odorant exposure. We glial projections infiltrate neuropil in response experience, and use Draper (MEGF10) engulfment receptors prune glomeruli. Downstream, antagonistic Basket (JNK) Puckered (DUSP) signaling required for translocation activated into nuclei. Dependent on this signaling, expression F-actin linking scaffold Cheerio (FLNA), absolutely essential pruning. mediates regulation cytoskeleton remodeling. These results define a sequential pathway strictly-defined period; input infiltration projections, Draper/MEGF10 activate Basket/JNK cascade transcriptional activation, Cheerio/FLNA induction regulates actin targeted phagocytosis.

Language: Английский

Citations

11
Ca2+ excitability of glia to neuromodulator octopamine in Drosophila living brain is greater than that of neurons DOI Creative Commons

Urška Černe,

Anemari Horvat,

Ena Sanjković

et al.

Acta Physiologica, Journal Year: 2025, Volume and Issue: 241(2)

Published: Jan. 13, 2025

Octopamine in the Drosophila brain has a neuromodulatory role similar to that of noradrenaline mammals. After release from Tdc2 neurons, octopamine/tyramine may trigger intracellular Ca We expressed Octopamine-stimulated In optic lobes, astrocytes, not appear be sole responders low concentration octopamine signals, and therefore likely drive synaptic plasticity visual processing. Given interconnectivity lobes with other regions, octopaminergic signals acting through lobe astrocytes also influence higher-order functions including learning memory.

Language: Английский

Citations

1
Parkinson’s disease-associated Pink1 loss disrupts vesicle trafficking in Ensheathing glia causing dopaminergic neuron synapse loss DOI Open Access

Lorenzo Ghezzi,

Ulrike Pech, Nils Schoovaerts

et al.

Published: Feb. 17, 2025

Parkinson’s disease (PD) is commonly associated with the loss of dopaminergic neurons in substantia nigra , but many other cell types are affected even before neuron occurs. Recent studies have linked oligodendrocytes to early stages PD, though their precise role still unclear. Pink1 mutated familial PD and through unbiased single-cell sequencing entire brain Drosophila models, we observed significant gene deregulation ensheathing glia (EG); cells that share functional similarities oligodendrocytes. We found leads activation EG, similar reactive response EG seen upon nerve injury. Using cell-type specific transcriptomics, identified deregulated genes as potential modifiers. Specifically, downregulating two trafficking factors, Rab7 Vps13, also or direct regulators Rab7, Mon1 Ccz1, specifically was sufficient rescue neuronal function protect against synapse loss. Our findings demonstrate triggers an injury turn disrupts function. Vesicle components, which regulate membrane interactions between organelles within play a crucial maintaining health preventing work highlights essential glial support pathogenesis identifies vesicle these key point convergence progression.

Language: Английский

Citations

1
In vivo autofluorescence lifetime imaging of the Drosophila brain captures metabolic shifts associated with memory formation DOI Open Access

Philémon Roussel,

Mingyi Zhou, Chiara Stringari

et al.

Published: March 31, 2025

Neuronal energy regulation is increasingly recognized as a critical factor underlying brain functions and their pathological alterations, yet the metabolic dynamics that accompany cognitive processes remain poorly understood. As label-free minimally invasive technique, fluorescence lifetime imaging (FLIM) of coenzymes NADH NADPH (collectively referred to NAD(P)H) offers possibility resolve cellular profiles with high spatial precision. However, NAD(P)H FLIM’s capacity detect subtle changes in neuronal metabolism associated cognition has not been demonstrated. In this study, we applied FLIM map Drosophila neurons vivo across multiple scales, focusing on primary centers for associative memory: mushroom bodies (MBs). At broad scale, obtained an overview signatures main tissue identified marked difference between neuropil cortex areas. finer our findings revealed notable heterogeneity basal distinct MB neuron subtypes. Measurements performed after olfactory learning also uncovered subtype-specific shift memory formation, demonstrating utility detecting physiology-driven linked function. These results establish promising framework studying cerebral vivo.

Language: Английский

Citations

1