Novel techniques to quantitatively assess age-dependent alterations in biophysical properties of HSPCs and bone marrow niche DOI Creative Commons
John D. Hayball, Motomu Tanaka

Experimental Hematology, Journal Year: 2024, Volume and Issue: unknown, P. 104686 - 104686

Published: Nov. 1, 2024

The present knowledge on hematopoietic stem and progenitor cell (HSPC) biology aging is based largely studies in mouse models. While models are invaluable, they not without limitations for defining how physical properties of HSPCs their niche change with age. bone marrow (BM) a complex, interactive environment multiple types. structure organization the BM niche, especially extracellular matrix (ECM), Provided recent advances quantitative analytical techniques vitro models, we have developed novel tools to quantitatively assess impact specific biochemical cues homing, adhesion migration HSPCs. Recent developments also provided new insights into interactions between particularly role stiffness. Further research needed integrate biomarkers comprehensive mathematical age-dependent HSPC-niche interactions. key use conjunction direct analyses achieve more understanding alterations function regulation.

Language: Английский

Skeletal stem and progenitor cells in bone physiology, ageing and disease DOI
Seppe Melis, Dana Trompet, Andrei S. Chagin

et al.

Nature Reviews Endocrinology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Language: Английский

Citations

7
Deciphering the Complexities of Adult Human Steady State and Stress-Induced Hematopoiesis: Progress and Challenges DOI Open Access
Suzanne M. Watt, Maria G. Roubelakis

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 671 - 671

Published: Jan. 14, 2025

Human hematopoietic stem cells (HSCs) have traditionally been viewed as self-renewing, multipotent with enormous potential in sustaining essential steady state blood and immune cell production throughout life. Indeed, around 86% (1011-1012) of new generated daily a healthy young human adult are origin. Therapeutically, HSCs contributed to over 1.5 million transplants (HCTs) globally, making this the most successful regenerative therapy date. We will commence review by briefly highlighting selected key achievements (from 1868 end 20th century) that accomplishment. Much our knowledge hematopoiesis is based on small animal models that, despite their importance, do not always recapitulate hematopoiesis. Given this, we critically progress challenges faced identifying tracing lineage differentiation trajectories, referring murine studies needed. Moving forward given dynamic can readily adjust variety stressors, then discuss recent research advances contributing understanding (i) which HSPCs maintain hematopoiesis, (ii) where these located, (iii) mechanisms come into play when homeostatic switches stress-induced or emergency

Language: Английский

Citations

0
Advances in Modern Medical Research on the Hematopoietic Effect of Qi and Blood Replenishing Drugs DOI

爽爽 段

Medical Diagnosis, Journal Year: 2025, Volume and Issue: 15(01), P. 123 - 133

Published: Jan. 1, 2025

Language: Английский

Citations

0
LGR4 (GPR48): The Emerging Inter-Bridge in Osteoimmunology DOI Creative Commons
Wonbong Lim

Biomedicines, Journal Year: 2025, Volume and Issue: 13(3), P. 607 - 607

Published: March 2, 2025

Leucine-rich repeat-containing G-protein-coupled receptor 4 (LGR4), a member of the (GPCR) family, has been implicated in various regulatory functions across multiple differentiation stages and numerous target sites bone diseases. Therefore, LGR4 is potential regulator nuclear factor-κB ligand (RANKL) during osteoclast differentiation. However, comprehensive investigation its applications immunology lacking. This review discusses molecular characteristics, signaling pathways, role osteoimmunology, with particular focus on interactions RANKL differentiation, while identifying gaps that warrant further research.

Language: Английский

Citations

0
Untangling Ariadne’s Thread Within the Bone Marrow Maze: A Close-Up View of Stem/Progenitor Cells’ Interactome and Secretome DOI

Dimitrios Agas,

Maria Giovanna Sabbieti

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Loss of Immune Anatomy During Infectious Disease DOI
Paul M. Kaye

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Development and aging of resident endothelial stem cells in pre-existing blood vessels DOI Creative Commons

Fitriana Nur Rahmawati,

Nobuyuki Takakura

Experimental Hematology, Journal Year: 2025, Volume and Issue: unknown, P. 104795 - 104795

Published: April 1, 2025

Organ-specific somatic stem cells play an important role in supporting tissue turnover and facilitating regeneration upon injury. Hematopoietic are one of the most established organ-specific that have been frequently used for transplantation therapy. In recent years, there has a growing interest other cells, including vascular endothelial (VESCs). We previously reported on use CD157 CD200 as markers to isolate VESCs from adult mouse organs, particularly liver. this review, we aim summarize, based our previous research, how CD157⁺CD200⁺ liver develop fetal stage postnatal life, what transcriptional regulatory mechanisms govern them, change with aging.

Language: Английский

Citations

0
Cellular crosstalk in the bone marrow niche DOI Creative Commons

Zeqi Huang,

Zoya Iqbal,

Zhe Zhao

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 3, 2024

The bone marrow niche is a special microenvironment that comprises elements, including hematopoietic stem cells, osteoblasts, and endothelial helps maintain their characteristic functions. Here, we elaborate on the crosstalk between various cellular components, other cells in niche. We further explain mechanism of preserving equilibrium niche, which crucial for directional regulation reconstruction repair. Additionally, elucidate intercommunication among osteocytes, osteoblast maturation activation by lymphocytes, deficiency megakaryocytes can markedly impair formation, interaction macrophages mesenchymal Finally, discussed new immunotherapies tumors BM In this review, aimed to provide candid overview highlight concepts underlying unknown mechanisms hematopoiesis reconstruction. Thus, review may more comprehensive understanding role these improving function help identify therapeutic potential different diseases future.

Language: Английский

Citations

1
Limitations of the radiotheranostic concept in neuroendocrine tumors due to lineage-dependent somatostatin receptor expression on hematopoietic stem and progenitor cells DOI Creative Commons
Nghia Trong Nguyen, Min Yu, Jennifer Rivière

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Abstract Radiopharmaceutical therapy (RPT) has become an effective treatment option for neuroendocrine tumors (NETs) and castration-resistant prostate cancer is in clinical development a growing number of indications. One the major advantages theranostic RPT that distribution radiopharmaceuticals human body can be imaged, radiation doses to patient’s organs calculated. However, accurate dosimetry may fundamentally limited by microscopic heterogeneity radiopharmaceutical distribution. We developed fluorescent analogs somatostatin-receptor-subtype 2 (SSTR2) targeting Lutetium-177 labelled are clinically used patients with studied their uptake hematopoietic stem progenitor cells (HSPC). Hematopoietic (HSCs) multipotent (MPPs) showed high specific SSTR2-ligand uptake, which was at similar levels as tumor cells. Furthermore, they displayed several-fold higher SSTR2-antagonists than SSTR2-agonists. HSPC 177 Lu-labelled antagonist agonist stronger reduction HSC proliferation antagonist. Due scarcity HSCs MPPs, contribution total bone marrow SSTR2-ligands not detectable imaging-based studies. This likely explains why caused pancytopenia trials despite safe estimates. In conclusion, target expression lead underestimation toxicity should considered when designing new radiopharmaceuticals. The implications our findings go beyond somatostatin receptor-targeted suggest more generally first-in-human studies only guided from imaging but also include careful escalation administered therapeutic activity. MMC technology modular applied other peptide or protein-based study cellular potential prior testing.

Language: Английский

Citations

0
Novel techniques to quantitatively assess age-dependent alterations in biophysical properties of HSPCs and bone marrow niche DOI Creative Commons
John D. Hayball, Motomu Tanaka

Experimental Hematology, Journal Year: 2024, Volume and Issue: unknown, P. 104686 - 104686

Published: Nov. 1, 2024

The present knowledge on hematopoietic stem and progenitor cell (HSPC) biology aging is based largely studies in mouse models. While models are invaluable, they not without limitations for defining how physical properties of HSPCs their niche change with age. bone marrow (BM) a complex, interactive environment multiple types. structure organization the BM niche, especially extracellular matrix (ECM), Provided recent advances quantitative analytical techniques vitro models, we have developed novel tools to quantitatively assess impact specific biochemical cues homing, adhesion migration HSPCs. Recent developments also provided new insights into interactions between particularly role stiffness. Further research needed integrate biomarkers comprehensive mathematical age-dependent HSPC-niche interactions. key use conjunction direct analyses achieve more understanding alterations function regulation.

Language: Английский

Citations

0