Drosera rotundifolia L. as E. coli biofilm inhibitor: insights into the mechanism of action using proteomics/metabolomics and toxicity studies DOI Creative Commons

Sandy Gerschler,

Sandra Maaß,

Philip Gerth

et al.

Biofilm, Journal Year: 2025, Volume and Issue: 9, P. 100268 - 100268

Published: March 1, 2025

The successful sustainable cultivation of the well-known medicinal plant sundew on rewetted peatlands not only leads to preservation natural populations, but also provides a basis for pharmaceutical use plant. bioactive compounds sundew, flavonoids and naphthoquinones, show biofilm-inhibiting properties against multidrug-resistant, ESBL-producing E. coli strains open up new therapeutic possibilities. This study investigates molecular mechanisms these in biofilm inhibition through proteomic analyses. Specific fractions as well individual substances like 7-methyljuglone 2″-O-galloylhyperoside, are analyzed. Results that naphthoquinones appear act via central regulatory proteins such OmpR alter stress response while likely affect formation by creating an iron-poor environment iron complexation additionally influence polyamine balance, reducing intracellular spermidine levels. Further investigations including assays analysis polyamines confirmed data. Safety evaluations cytotoxicity tests 3D cell cultures Galleria mellonella vivo model confirm safety extracts used. These findings highlight promising candidate phytopharmaceuticals.

Language: Английский

Drosera rotundifolia L. as E. coli biofilm inhibitor: insights into the mechanism of action using proteomics/metabolomics and toxicity studies DOI Creative Commons

Sandy Gerschler,

Sandra Maaß,

Philip Gerth

et al.

Biofilm, Journal Year: 2025, Volume and Issue: 9, P. 100268 - 100268

Published: March 1, 2025

The successful sustainable cultivation of the well-known medicinal plant sundew on rewetted peatlands not only leads to preservation natural populations, but also provides a basis for pharmaceutical use plant. bioactive compounds sundew, flavonoids and naphthoquinones, show biofilm-inhibiting properties against multidrug-resistant, ESBL-producing E. coli strains open up new therapeutic possibilities. This study investigates molecular mechanisms these in biofilm inhibition through proteomic analyses. Specific fractions as well individual substances like 7-methyljuglone 2″-O-galloylhyperoside, are analyzed. Results that naphthoquinones appear act via central regulatory proteins such OmpR alter stress response while likely affect formation by creating an iron-poor environment iron complexation additionally influence polyamine balance, reducing intracellular spermidine levels. Further investigations including assays analysis polyamines confirmed data. Safety evaluations cytotoxicity tests 3D cell cultures Galleria mellonella vivo model confirm safety extracts used. These findings highlight promising candidate phytopharmaceuticals.

Language: Английский

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