DIA/SWATH-Mass Spectrometry Revealing Melanoma Cell Proteome Transformations with Silver Nanoparticles: An Innovative Comparative Study DOI Open Access
Simona Martano, Jakub Faktor, Sachin Kote

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2029 - 2029

Published: Feb. 26, 2025

Melanoma is an aggressive cancer with rising incidence and high mortality rates, largely due to chemotherapy resistance molecular dysregulation. Nanotechnology, particularly silver nanoparticles (AgNPs), has emerged as a promising therapeutic avenue because of the nanoparticles' ability induce oxidative stress apoptosis in cells. However, conventional colloidal AgNPs lack selectivity, often causing significant damage healthy In this study, we introduce green synthesis using plant extracts, providing eco-friendly alternative improved antitumor selectivity compared traditional AgNPs. Leveraging label-free Data-Independent Acquisition/Sequential Window Acquisition All Theoretical Mass Spectrometry (DIA/SWATH MS) quantitative proteomics, investigated effects green-synthesized versus on A375 melanoma cells at 24 48 h. Our findings reveal that selectively reduced cell viability while sparing keratinocytes (HaCaT), benefit not observed Proteomic analysis highlighted significantly downregulated oncogenes, enhanced carbohydrate metabolism, disrupted copper homeostasis This marks first study explore differential integrated proteomic approach, underscoring potential targeted sustainable option for therapy.

Language: Английский

DIA/SWATH-Mass Spectrometry Revealing Melanoma Cell Proteome Transformations with Silver Nanoparticles: An Innovative Comparative Study DOI Open Access
Simona Martano, Jakub Faktor, Sachin Kote

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2029 - 2029

Published: Feb. 26, 2025

Melanoma is an aggressive cancer with rising incidence and high mortality rates, largely due to chemotherapy resistance molecular dysregulation. Nanotechnology, particularly silver nanoparticles (AgNPs), has emerged as a promising therapeutic avenue because of the nanoparticles' ability induce oxidative stress apoptosis in cells. However, conventional colloidal AgNPs lack selectivity, often causing significant damage healthy In this study, we introduce green synthesis using plant extracts, providing eco-friendly alternative improved antitumor selectivity compared traditional AgNPs. Leveraging label-free Data-Independent Acquisition/Sequential Window Acquisition All Theoretical Mass Spectrometry (DIA/SWATH MS) quantitative proteomics, investigated effects green-synthesized versus on A375 melanoma cells at 24 48 h. Our findings reveal that selectively reduced cell viability while sparing keratinocytes (HaCaT), benefit not observed Proteomic analysis highlighted significantly downregulated oncogenes, enhanced carbohydrate metabolism, disrupted copper homeostasis This marks first study explore differential integrated proteomic approach, underscoring potential targeted sustainable option for therapy.

Language: Английский

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