Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)
Published: Oct. 9, 2024
Pulmonary hypertension (PH) is marked by elevated pulmonary artery pressures due to various causes, impacting right heart function and survival. Disulfidptosis, a newly recognized cell death mechanism, may play role in PH, but its associated genes (DiGs) are not well understood this context. This study aims define the diagnostic relevance of DiGs PH.
Language: Английский
The FASEB Journal, Journal Year: 2025, Volume and Issue: 39(3)
Published: Feb. 9, 2025
Bacteria in dental plaque invade periodontal tissues, causing chronic inflammation known as periodontitis. Despite advancements understanding periodontitis, its molecular pathogenesis remains incompletely elucidated. In this study, a total of 247 samples were retrieved from the Gene Expression Omnibus (GEO) database, comprising 183 individuals with periodontitis and 64 healthy controls. Differentially expressed DRGs (DE-DRGs) identified, their expression correlations analyzed. Immune cell infiltration association DE-DRGs assessed. Set Variation Analysis (GSVA) was performed to determine key functions pathways related DE-DRGs. Characteristic (CDE-DRGs) identified using Least Absolute Shrinkage Selection Operator (LASSO) analysis, risk model personalized nomogram constructed. Model performance validated through calibration decision curve analysis (DCA). External experiments, including qRT-PCR Western blot, confirmed differential Fourteen identified. revealed strong synergistic correlation between MYH9 ACTB (coefficient = 0.86) an antagonistic NCKAP1 FLNA -0.52). profiling showed significant differences proportions 22 immune types groups, 14 correlated levels. Cluster distinct patterns across two clusters. A incorporating four CDE-DRGs (DSTN, SLC7A11, SLC3A2, RPN1) developed, alongside for predicting risk. blot analyses demonstrated downregulation DSTN, IQGAP1, CD2AP, upregulation RPN1, FLNA, MYH9, TLN1, ACTB, MYH10, CAPZB, PDLIM1 tissues. This study developed predictive nomogram, detailed profile DRGs. These findings provide insights into suggest potential strategies assessment, early diagnosis, targeted therapy.
Language: Английский
Citations
0
Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(5)
Published: March 1, 2025
ABSTRACT Ischaemic cardiomyopathy (IC) predominantly arises from prolonged deprivation of oxygen in the coronary arteries, resulting compromised cardiac contractility or relaxation. This study investigates role disulfidptosis‐associated genes (DiGs) IC. Through analysis datasets GSE5406 and GSE57338, we explored association between DiGs immune characteristics to identify crucial contributing IC development. The support vector machine model emerged as most effective, identifying key such MYH9, NUBPL, MYL6, MYH10 NCKAP1. Validation with independent GSE57345, GSE48166 single‐cell GSE145154 further supported these findings, demonstrating high predictive accuracy. Experimental validation an mouse model, using Western blot, immunohistochemistry RT‐qPCR, confirmed altered expression core myocardial ischaemic regions. research not only elucidates significance but also underscores diagnostic potential identified genes.
Language: Английский
Citations
0
Immuno, Journal Year: 2025, Volume and Issue: 5(2), P. 13 - 13
Published: April 15, 2025
Chronic obstructive pulmonary disease (COPD) is a chronic, debilitating condition that affects the lungs and airways. It characterized by persistent bronchitis, exemplified inflammation of bronchial tubes, hypersecretion mucus, emphysema, destruction airway parenchyma. The combination these conditions leads to tissue damage, fibrosis, ongoing inflammatory response in COPD complex process orchestrated wide range immune cells. These include lung epithelial cells, monocytes, macrophages, neutrophils, eosinophils, T B lymphocytes, among others. cells work together produce biomarkers are involved pathogenesis COPD. Some key have been identified variety cytokines, C-reactive protein/serum albumin ratio, fibrinogen, soluble receptor for advanced glycation endproducts, club/clara with molecular weight 16 kDa, surfactant protein D, adiponectin, reactive oxygen species, proteases. This review aims provide comprehensive overview role development progression will delve into intricacies COPD, exploring various cell types this process. By understanding underlying mechanisms drive we can better develop targeted treatments help alleviate symptoms
Language: Английский
Citations
0
Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)
Published: Oct. 9, 2024
Pulmonary hypertension (PH) is marked by elevated pulmonary artery pressures due to various causes, impacting right heart function and survival. Disulfidptosis, a newly recognized cell death mechanism, may play role in PH, but its associated genes (DiGs) are not well understood this context. This study aims define the diagnostic relevance of DiGs PH.
Language: Английский
Citations
0