Identification of key genes with abnormal RNA methylation modification and selected m6A regulators in ankylosing spondylitis DOI Creative Commons

Fengqing Wu,

Hongbin Huang, Deyang Sun

et al.

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(8)

Published: Aug. 1, 2024

Abstract Background N6‐methyladenosine (m6A) has been identified as the most abundant modification of RNA molecules and aberrant m6A modifications have associated with development autoimmune diseases. However, role in ankylosing spondylitis (AS) not adequately investigated. Therefore, we aimed to explore significance regulator‐mediated methylation AS. Methods The methylated immunoprecipitation sequencing (meRIP‐seq) digital (Digital RNA‐seq) were conducted using peripheral blood mononuclear cells from three AS cases healthy controls, identify genes affected by abnormal methylation. different peaks cross‐referenced AS‐related obtained GeneCards Suite. Subsequently, expression levels shared differentially expressed (DEGs) key regulators evaluated data 68 36 controls two sets (GSE25101 GSE73754). In addition, results validated through quantitative polymerase chain reaction (qPCR). Results meRIP‐seq Digital RNA‐seq analyses 28 upregulated but downregulated expression, 52 expression. By intersecting 2184 Suite, a total five DEGs: BCL11B , KAT6B IL1R1 TRIB1 ALDH2 . Through analysis qPCR, found that Moreover, regulators, WTAP heterogeneous nuclear ribonucleoprotein C, identified. Conclusions conclusion, current study revealed plays crucial might hence provide new treatment strategy for disease.

Language: Английский

Identification of key genes with abnormal RNA methylation modification and selected m6A regulators in ankylosing spondylitis DOI Creative Commons

Fengqing Wu,

Hongbin Huang, Deyang Sun

et al.

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(8)

Published: Aug. 1, 2024

Abstract Background N6‐methyladenosine (m6A) has been identified as the most abundant modification of RNA molecules and aberrant m6A modifications have associated with development autoimmune diseases. However, role in ankylosing spondylitis (AS) not adequately investigated. Therefore, we aimed to explore significance regulator‐mediated methylation AS. Methods The methylated immunoprecipitation sequencing (meRIP‐seq) digital (Digital RNA‐seq) were conducted using peripheral blood mononuclear cells from three AS cases healthy controls, identify genes affected by abnormal methylation. different peaks cross‐referenced AS‐related obtained GeneCards Suite. Subsequently, expression levels shared differentially expressed (DEGs) key regulators evaluated data 68 36 controls two sets (GSE25101 GSE73754). In addition, results validated through quantitative polymerase chain reaction (qPCR). Results meRIP‐seq Digital RNA‐seq analyses 28 upregulated but downregulated expression, 52 expression. By intersecting 2184 Suite, a total five DEGs: BCL11B , KAT6B IL1R1 TRIB1 ALDH2 . Through analysis qPCR, found that Moreover, regulators, WTAP heterogeneous nuclear ribonucleoprotein C, identified. Conclusions conclusion, current study revealed plays crucial might hence provide new treatment strategy for disease.

Language: Английский

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