Platelet-Derived Soluble CD40L and Its Impact on Immune Modulation and Anti-IL6R Antibody Treatment Outcome in Rheumatoid Arthritis DOI Creative Commons
Carlos Zamora, Cèsar Díaz‐Torné, M. Angels Ortiz

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(9), P. 625 - 625

Published: April 22, 2025

Background: Platelets (PLTs) from healthy donors (HD) modulate T lymphocyte responses but PLTs rheumatoid arthritis (RA) patients contribute to persistent systemic inflammation. This suggests that RA and HD have different immunomodulatory effects. Methods: Using cell culture, flow cytometry, proteomics, ELISA, we compared their effects on activation cytokine production. Results: suppressed proliferation IFNγ TNF production, while exhibited reduced suppressive capacity. In the presence of PLTs, levels correlated with proliferation, greater disease activity, anti-citrullinated protein antibodies (ACPA). Proteomic analysis revealed show upregulation proteins linked acute-phase response complement activation. secreted higher soluble CD40L (sCD40L) PDGF-BB enhanced Seropositive had sCD40L, these were predictive remission in treated anti-IL6R. sCD40L was found enhance increased pro-inflammatory Conclusions: study highlights diminished ability suppress can be a potential biomarker therapeutic target RA.

Language: Английский

Platelet-Derived Soluble CD40L and Its Impact on Immune Modulation and Anti-IL6R Antibody Treatment Outcome in Rheumatoid Arthritis DOI Creative Commons
Carlos Zamora, Cèsar Díaz‐Torné, M. Angels Ortiz

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(9), P. 625 - 625

Published: April 22, 2025

Background: Platelets (PLTs) from healthy donors (HD) modulate T lymphocyte responses but PLTs rheumatoid arthritis (RA) patients contribute to persistent systemic inflammation. This suggests that RA and HD have different immunomodulatory effects. Methods: Using cell culture, flow cytometry, proteomics, ELISA, we compared their effects on activation cytokine production. Results: suppressed proliferation IFNγ TNF production, while exhibited reduced suppressive capacity. In the presence of PLTs, levels correlated with proliferation, greater disease activity, anti-citrullinated protein antibodies (ACPA). Proteomic analysis revealed show upregulation proteins linked acute-phase response complement activation. secreted higher soluble CD40L (sCD40L) PDGF-BB enhanced Seropositive had sCD40L, these were predictive remission in treated anti-IL6R. sCD40L was found enhance increased pro-inflammatory Conclusions: study highlights diminished ability suppress can be a potential biomarker therapeutic target RA.

Language: Английский

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