Identification and Experimental Validation of Biomarkers Associated With Mitochondria and Macrophage Polarization in Sepsis

Emergency Medicine International, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background: Sepsis is a common and serious condition, where mitochondria macrophage polarization play crucial role. Therefore, this study aimed to identify validate biomarkers for sepsis associated with mitochondria‐related genes (MCRGs) polarization–related (MPRGs), providing new targets strategies therapeutic intervention. Methods: This utilized the GSE95233 GSE28750 datasets. Initially, intersection were identified by overlapping MCRGs results from differential expression analysis weighted gene co‐expression network (WGCNA). Biomarkers through machine learning analysis. A nomogram was developed evaluated based on these biomarkers. Finally, functional enrichment, immune infiltration, reverse transcription quantitative polymerase chain reaction (RT‐qPCR) analyses conducted further elucidate biological mechanisms underlying sepsis. Results: The YME1L1, ECHDC3, THEM4, COQ10A as Among them, showed significantly lower levels in samples, while ECHDC3 exhibited markedly higher expression. Notably, RT‐qPCR confirmed that samples. validated, effectively predicting risk. Enrichment indicated co‐enriched oxidative phosphorylation pathway. Additionally, 13 different cell types between control Biomarker association revealed CD8 T cells had strongest positive correlation YME1L1 (cor = 0.84, p < 0.05) negative −0.76, 0.05), suggesting their potential role disease mechanism. Conclusion: In study, sepsis, validated clinical These findings provided promising theoretical foundation development of targeted treatments

Language: Английский

Macrophages in the inflammatory response to endotoxic shock

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(10)

Published: Oct. 1, 2024

Abstract Background Endotoxic shock, particularly prevalent in intensive care units, represents a significant medical challenge. Endotoxin, upon invading the host, triggers intricate interactions with innate immune system, macrophages. This activation leads to production of inflammatory mediators such as tumor necrosis factor‐alpha, interleukin‐6, and interleukin‐1‐beta, well aberrant nuclear factor‐kappa‐B mitogen‐activated protein kinase signaling pathways. Objective review delves into cascades underpinning endotoxic particular focus on pivotal role It aims elucidate clinical implications these processes offer insights potential therapeutic strategies. Results Macrophages, central regulation, manifest two distinct subsets: M1 (classically activated subtype) macrophages M2 (alternatively The former exhibit an phenotype, while latter adopt anti‐inflammatory role. By modulating response patients play crucial restoring balance facilitating recovery. Conclusion Macrophages undergo dynamic changes within orchestrating essential for maintaining tissue homeostasis. A deeper comprehension mechanisms governing macrophage‐mediated inflammation lays groundwork anti‐inflammatory, targeted approach treating shock. understanding can significantly contribute development more effective interventions.

Language: Английский

Gelatin Extracted From Yanbian Cattle Skin Suppresses LPS‐Induced Inflammation in RAW264.7 Cells

Journal of Food Biochemistry, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Objective: To develop an efficient method for extracting gelatin from Yanbian cattle skin and to study its anti‐inflammatory effects. Methods: Gelatin was extracted using enzymatic hydrolysis water extraction techniques. The basic structures of the prepared were analyzed Fourier transform infrared spectroscopy (FT‐IR), ultraviolet (UV) spectroscopy, amino acid analysis, scanning electron microscopy (SEM), matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS). LPS established RAW264.7 cell inflammation model in vitro. therapeutic dose range gelatin, which did not exhibit significant cytotoxicity, determined CCK‐8 assay subsequent experiments. nitric oxide (NO) content measured Griess method. proinflammatory cytokines ELISA. Reactive oxygen species (ROS) levels detected DCFH‐DA fluorescent probe method, oxidative stress–related indicators a kit. mRNA expression inducible synthase (iNOS), cyclooxygenase‐2 (COX‐2), qRT‐PCR. Western blot (WB) used detect protein iNOS COX‐2 each group. Results: Characteristic absorption peaks observed FT‐IR UV spectra. Additionally, significantly reduced NO cells, decreased secretion cytokines, inhibited ROS generation, indicators, lowered iNOS, COX‐2, iNOS. Conclusion: Enzymatic efficiently prepare skin. This can inhibit LPS‐induced potentially by inhibiting ameliorating stress.

Language: Английский