Integrated multi-omics analysis of the microbial profile characteristics associated with pulmonary arterial hypertension in congenital heart disease DOI Creative Commons

Jiahui Xie,

Xiaoyu Zhang, Liming Cheng

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

ABSTRACT Dysregulation of immune and inflammatory cells around blood vessels metabolic dysfunction are key mechanisms in the development pulmonary arterial hypertension (PAH). The homeostasis human microbiome plays a crucial role regulating responses progression diseases. For associated with congenital heart disease involving body-lung shunt (PAH-CHD), potential impact on “gut-lung axis” remains underexplored. This study recruited 15 healthy individuals patients due to from Fuwai Yunnan Hospital, Chinese Academy Medical Sciences, Kunming Children's Hospital. We performed differential analyses metabolites microbiota both gut lower respiratory tract for these two groups. goal was investigate metabolome profiles children PAH-CHD analyze interrelationships between profiles. Ultimately, we aim propose value aiding diagnosis. results indicated that characterized by an increased abundance beneficial symbionts, which closely linked changes metabolome. Metabolite functional enrichment analysis revealed energy metabolism reprogramming group, active pathways bile acid secretion carnitine homeostasis. Moreover, expression correlated right function growth development. IMPORTANCE Previous studies have primarily focused relationship PAH. However, microbial perspective gut-lung axis has not been adequately elucidated. Our utilizes integrated multi-omics approach report characteristics lung reference subjects. found influence pathological manifestations through their activity. Additionally, alterations ecological structure. findings suggest modulating composition may positive implications maintaining environment PAH-CHD.

Language: Английский

Prophylactic supplementation with Bifidobacterium infantis or its metabolite inosine attenuates cardiac ischemia/reperfusion injury DOI Creative Commons
Hao Zhang, Jiawan Wang, Jianghua Shen

et al.

iMeta, Journal Year: 2024, Volume and Issue: 3(4)

Published: July 2, 2024

Abstract Emerging evidence has demonstrated the profound impact of gut microbiome on cardiovascular diseases through production diverse metabolites. Using an animal model myocardial ischemia–reperfusion (I/R) injury, we found that prophylactic administration a well‐known probiotic, Bifidobacterium infantis ( B. ), exhibited cardioprotective effects in terms preserving cardiac contractile function and preventing adverse remodeling following I/R these were recapitulated by its metabolite inosine. Transcriptomic analysis further revealed inosine mitigated I/R‐induced inflammation cell death. Mechanistic investigations elucidated suppressed pro‐inflammatory cytokines reduced numbers dendritic cells natural killer cells, achieved activation adenosine A2A receptor (A2AR) when inhibited abrogated Additionally, vitro studies using C2C12 myoblasts attenuated death serving as alternative carbon source for triphosphate (ATP) generation purine salvage pathway subjected to oxygen‐glucose deprivation/reoxygenation simulated injury. Likewise, reversed decrease ATP levels mouse hearts. Taken together, our findings indicate or exerts against suppressing attenuating death, suggesting therapeutic options acute ischemic

Language: Английский

Citations

8
The role and mechanism of gut-lung axis mediated bidirectional communication in the occurrence and development of chronic obstructive pulmonary disease DOI Creative Commons

Xiaofan Song,

Xina Dou,

Jiajing Chang

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Oct. 24, 2024

The current studies have shown that the occurrence and development of chronic obstructive pulmonary disease (COPD) are closely related to changes in gut health its microenvironment, even some diseases significant clinical correlation with COPD. dysbiosis microbiota observed COPD patients also suggests a potential bidirectional interaction between lung. Communication lung may occur through circulating inflammatory cells, microbial metabolites, mediators, but mechanism communication is still under study. Therefore, more research needed this area. In review, we summarize recent animal models on role gut-lung axis mechanisms, so as provide ideas for further field. addition, summarized negative effects medication risk factors proposed prevention treatment strategies.

Language: Английский

Citations

4
Exploring the Causal Relationship Between Gut Microbiota and Pulmonary Artery Hypertension: Insights From Mendelian Randomization DOI Creative Commons
Lihuang Su, Xinghong Wang,

Ya Lin

et al.

Journal of the American Heart Association, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Background Research into the “gut–lung” axis links gut microbiota to pulmonary artery hypertension (PAH). However, mechanisms by which influence PAH remain unclear. We aimed investigate causal relationship between and using Mendelian randomization analysis, identify key metabolites, explore regulatory role of associated genes in pathogenesis. Methods Results examined association taxa inverse variance weighted 2‐sample randomization–Egger, median, mode methods. Additionally, we identified PAH‐regulating intestinal microbiome bioinformatics tools validated their expression levels lung tissue hypoxia‐induced mice models quantitative reverse transcription polymerase chain reaction. Eleven were with PAH. The order Clostridiales genera Eubacterium fissicatena group, Lachnospiraceae UCG004 , Ruminococcaceae UCG002 positively PAH, whereas Bifidobacteriales; family Bifidobacteriaceae; eligens group Sutterella, Methanobrevibacter Sellimonas Tyzzerella3 negatively some exhibiting bidirectional causality. These modulate 24 including palmitoylcholine, oleoylcholine, 3,7‐dimethylurate, Hypoxia‐induced had significantly downregulated 1,4,5‐trisphosphate receptor type 2, degrading enzyme, nuclear receptor‐interacting protein 1, growth factor‐binding 1 tissues, indicating potential regulation. Conclusions findings suggest that composition metabolites contribute development regulating gene expression. Our have implications for advancing microbiota‐based diagnostic technologies targeted therapies.

Language: Английский

Citations

0
Gut-lung Axis mediates asthma pathogenesis: Roles of dietary patterns and their impact on the gut microbiota DOI Creative Commons
Yanbo Liu, Ying Zhou, Haoyue Zhang

et al.

Experimental and Molecular Pathology, Journal Year: 2025, Volume and Issue: 142, P. 104964 - 104964

Published: April 8, 2025

The gut-lung axis, a vital signaling network linking the gastrointestinal and pulmonary systems, regulates immune responses progression of respiratory diseases. Nutritional components can modulate gut microbiome regulate synthesis critical intestinal microbial metabolites, which are essential for maintaining homeostasis supporting health. Conversely, poor dietary habits exacerbate asthma other conditions through modulation systemic inflammation responses. Dietary interventions, such as Mediterranean diet, reported to restore balance improve health by increasing production anti-inflammatory potentiating responses, preserving epithelial barrier integrity. In contrast, Western patterns, characterized high fat low fiber intake, disrupt diversity, resulting in increased levels pro-inflammatory metabolites that aggravate airway severity. This review aimed elucidate mechanisms underlying regulatory effects microbes their on asthma. Additionally, previous findings related axis have been summarized, providing insights into potential therapeutic strategies management.

Language: Английский

Citations

0
Multi-kingdom gut microbiota dysbiosis is associated with the development of pulmonary arterial hypertension DOI

Yihang Chen,

Zhenzhen Chen,

Lirong Liang

et al.

EBioMedicine, Journal Year: 2025, Volume and Issue: 115, P. 105686 - 105686

Published: April 11, 2025

Language: Английский

Citations

0
Metformin’s dual impact on Gut microbiota and cardiovascular health: A comprehensive analysis DOI Open Access
Turky Omar Asar, Fahad A. Al‐Abbasi, Ryan A. Sheikh

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 178, P. 117128 - 117128

Published: July 29, 2024

Cardiovascular diseases (CVD) cause significant global morbidity, mortality and public health burden annually. CVD alters richness, diversity, composition of Gut microbiota along with RAS histopathological differences. Present study explores Metformin role in mitigating doxorubicin induced cardiovascular toxicity/remodeling. Animals were divided into 4 groups n=6: Group I (N. Control) free access to diet water; II (MET. on oral (250 mg/kg) daily; III (DOX. alternate day intraperitoneal Doxorubicin (3 totaling 18 mg/kg; IV MET. received both daily mg/kg). microbial analysis was made from stool before animals sacrificed for biochemical analysis. Significant alterations observed ɑ β-diversity new genus Firmicutes, specifically Clostridia_UCG-014, Eubacterium ruminantium, Tunicibacter, prevalent the DOX. Control DOX.MET groups. Proteobacteria, represented by Succinivibrio, absent all Additionally, Parabacteroides Bacteroidia phylum except N. control. In group, levels Angiotensin ( 7.75± 0.49 nmol/min, p<0.01) Renin (2.60±0.26 ng/ml/hr) significantly reduced. Conversely, CK-MB, Fibrinogen, Troponin, CRP p < 0.0001), TNFɑ (p 0.05) elevated. Histopathological examination revealed substantial cardiac changes, including Fibrinogen fat deposition eosinophilic infiltration, as well liver damage characterized binucleated cells damaged hepatocytes, altered renal tissues DOX.MET.Control group. The findings suggest that modifies gut microbiota, particularly impacting Firmicutes Proteobacteria phyla. reduction levels, alongside increased inflammatory markers myocardial damage, highlights complex interactions potential adverse effects associated MET therapy health.

Language: Английский

Citations

3
Flavonifractor Plautii or Its Metabolite Desaminotyrosine as Prophylactic Agents for Alleviating Myocardial Ischemia/Reperfusion Injury DOI Creative Commons
Heng Du, Xu Liu, Jianghua Shen

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

Abstract Myocardial ischemia/reperfusion (I/R) injury is a major contributor to myocardial damage, leading adverse cardiac remodeling and dysfunction. Recent studies have highlighted the potential of gut microbiota‐derived metabolites in modulating outcomes. Here, cardioprotective effects commensal bacterium Flavonifractor plautii ( F. ) its metabolite desaminotyrosine (DAT) against I/R are investigated. We showed that prophylactic gavage attenuates as evidenced by improved function reduced injury. also found DAT recapitulates these Transcriptomic analysis has revealed preserves tissue immune responses Mechanistically, promotes cardiomyocyte survival through modulation nicotinamide adenine dinucleotide phosphate (NADP + /NADPH) ratio. Further, suppressed macrophage proinflammatory activities inflammation via reduction interleukin‐6 (IL‐6) production. Taken together, our findings indicate exert pleiotropic injury, suggesting them therapeutic options for alleviating

Language: Английский

Citations

0
The Challenge in Burden of Pulmonary Arterial Hypertension: A Perspective From the Global Burden of Disease Study DOI Creative Commons
Yicheng Yang, Zhiwei Zeng,

Qiaoxi Yang

et al.

MedComm, Journal Year: 2025, Volume and Issue: 6(5)

Published: April 24, 2025

ABSTRACT Pulmonary arterial hypertension (PAH) poses significant clinical management challenges due to gaps in understanding its global epidemiology. We analyzed PAH‐related disability‐adjusted life years (DALYs), deaths, and prevalence from 1990 2021. Age‐period‐cohort models regression analyses assessed temporal trends projected burdens 2050. Globally, DALYs declined by 6.6%, but increased 13.9% high socio‐demographic index (SDI) countries. Middle SDI regions reported the highest Deaths rose 48.5% worldwide, with nations experiencing a 76.6% surge. Age‐standardized rates (ASRs) of deaths decreased across countries, high‐middle showing steepest declines. Younger age groups, especially males, had higher proportion earlier years, burden shifted toward older populations over time, this trend more pronounced high‐SDI analysis revealed declining younger ages rising cohorts. By 2050, are rise, disproportionately affecting females. Significant regional disparities PAH persist, necessitating targeted policies, improved healthcare access, early detection strategies, underserved areas. Addressing these is critical for mitigating PAH’ s impact.

Language: Английский

Citations

0
Integrated multi-omics analysis of the microbial profile characteristics associated with pulmonary arterial hypertension in congenital heart disease DOI Creative Commons

Jiahui Xie,

Xiaoyu Zhang, Liming Cheng

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

ABSTRACT Dysregulation of immune and inflammatory cells around blood vessels metabolic dysfunction are key mechanisms in the development pulmonary arterial hypertension (PAH). The homeostasis human microbiome plays a crucial role regulating responses progression diseases. For associated with congenital heart disease involving body-lung shunt (PAH-CHD), potential impact on “gut-lung axis” remains underexplored. This study recruited 15 healthy individuals patients due to from Fuwai Yunnan Hospital, Chinese Academy Medical Sciences, Kunming Children's Hospital. We performed differential analyses metabolites microbiota both gut lower respiratory tract for these two groups. goal was investigate metabolome profiles children PAH-CHD analyze interrelationships between profiles. Ultimately, we aim propose value aiding diagnosis. results indicated that characterized by an increased abundance beneficial symbionts, which closely linked changes metabolome. Metabolite functional enrichment analysis revealed energy metabolism reprogramming group, active pathways bile acid secretion carnitine homeostasis. Moreover, expression correlated right function growth development. IMPORTANCE Previous studies have primarily focused relationship PAH. However, microbial perspective gut-lung axis has not been adequately elucidated. Our utilizes integrated multi-omics approach report characteristics lung reference subjects. found influence pathological manifestations through their activity. Additionally, alterations ecological structure. findings suggest modulating composition may positive implications maintaining environment PAH-CHD.

Language: Английский

Citations

0