Journal of Environmental Sciences, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
Language: Английский
The Innovation, Journal Year: 2024, Volume and Issue: 5(4), P. 100612 - 100612
Published: March 13, 2024
Language: Английский
Citations
154
iMeta, Journal Year: 2023, Volume and Issue: 2(1)
Published: Jan. 31, 2023
Bacterial pathogens are one of the major threats to biosafety and environmental health, advanced assessment is a prerequisite combating bacterial pathogens. Currently, 16S rRNA gene sequencing efficient in open-view detection However, taxonomic resolution applicability this method limited by domain-specific pathogen database, profiling method, target variable regions. Here, we present pipeline multiple (MBPD) identify animal, plant, zoonotic MBPD based on large, curated database full-length genes 1986 reported species covering 72,685 sequences. In silico comparison allowed provide appropriate similarity threshold for both variable-region platforms, while subregion V3-V4 (mean: 88.37%, accuracy rate compared V1-V9) outperformed other regions identification sequencing. Benchmarking real data sets suggested superiority broader range detections with methods, including 16SPIP MIP. Beyond detecting known causal agent human, plant diseases, capable identifying cocontaminating from biological samples. Overall, agricultural, veterinary, medical, monitoring achieve One Health.
Language: Английский
Citations
60
The Innovation, Journal Year: 2023, Volume and Issue: 5(1), P. 100543 - 100543
Published: Nov. 22, 2023
Public summary•The plastisphere selectively assembles a microbiome distinct from that of natural habitats.•New microbial coexistence patterns are yielded in the plastisphere.•Altered functions threaten ecosystem functioning.•Enrichment pathogens poses critical challenge to "One Health".AbstractPlastic offers new niche for microorganisms, plastisphere. The ever-increasing emission plastic waste makes it understand ecology and associated effects. Here, we present global fingerprint plastisphere, analyzing samples collected freshwater, seawater, terrestrial ecosystems. community has clearly higher heterogeneity more deterministically dominated assembly compared habitats. New patterns—loose fragile networks with mostly specialist linkages among microorganisms rarely found habitats—are seen Plastisphere microbiomes generally have great potential metabolize organic compounds, which could accelerate carbon turnover. Microorganisms involved nitrogen cycle also altered especially freshwater plastispheres, where high abundance denitrifiers may increase release nitrite (aquatic toxicant) nitrous oxide (greenhouse gas). Enrichment animal, plant, human means become an increasingly mobile reservoir harmful microorganisms. Our findings highlight if trajectory emissions is not reversed, expanding pose planetary health challenges.Graphical abstract
Language: Английский
Citations
58
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 28, 2025
Abstract Despite advances in dispersal mechanisms and risk assessment of antibiotic resistance genes (ARGs), how plants influence ARG contamination agricultural soils remains underexplored. Here, the impacts plant species diversity on ARGs mobile genetic elements (MGEs) three are comprehensively investigated a pot experiment. The results indicate that increased reduces MGEs abundance by 19.2%–51.2%, whereas exhibit inconsistent soil‐dependent effects. Potential bacterial hosts harboring abundant have greater relative than nonhosts, both their richness cumulative reduced diversity. Notably, inhibited present other hosts. enriched compounds root exudates due to play more important role metabolic network contribute rebalancing potential nonhosts. An independent test using pure organics reveals higher resource diversity, resulting from mobility high‐risk ARGs. This study highlights resource‐mediated mitigation risks posed indicates ensuring is promising strategy for controlling agroecosystems.
Language: Английский
Citations
3
Nature Food, Journal Year: 2023, Volume and Issue: 4(11), P. 996 - 1006
Published: Oct. 30, 2023
Language: Английский
Citations
39
Water Research, Journal Year: 2024, Volume and Issue: 253, P. 121305 - 121305
Published: Feb. 12, 2024
Language: Английский
Citations
14
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 913, P. 169767 - 169767
Published: Jan. 2, 2024
Inadequately managed solid organic waste generation poses a threat to the environment and human health globally. Biotransformation with black soldier fly larvae (BSFL) is emerging as talent technology for management However, there lack of understanding whether BSFL can effectively suppress potential pathogenic microorganisms during underlying mechanisms. In this study, we investigated temporal variations in two common types waste, i.e., kitchen (KW) pig manure (PM). Natural composting under three different pH levels (pH 5, 7, 9) were established explore their impact on microbial communities compost gut BSFL. The results showed that led an increase relative abundance various potentially bacteria. Temporal gradient analyses revealed most substantial reduction diversity occurred when initial both wastes adjusted 7 upon introduction Through network pls-pm analysis, it was discovered microbiota occupied ecological niche compost, inhibiting proliferation microorganisms. This study has reducing public risks process, providing robust support sustainable management.
Language: Английский
Citations
10
One Health, Journal Year: 2025, Volume and Issue: 20, P. 100973 - 100973
Published: Jan. 13, 2025
Oyster aquaculture world-wide is a booming industry that can provide many benefits to coastal habitats, including economic, ecosystem-level, and cultural benefits. Oysters present several risks for human consumption, transmission of parasites, bacterial viral pathogens. microbiomes are well-defined, but their connection the incidence pathogens, humans or others, unclear. Furthermore, viruses associated with oysters largely unknown, humans, animals, ecosystem health has not been explored. Here, we employed One Health framework modern molecular techniques, 16S rRNA amplicon metagenomic sequencing, identify links between changes in microbial communities pathogens detected oyster tissues surrounding environments. In addition, adapted BioFire® FilmArray®, commonly used hospitals, determine presence within sampled oysters. We known 50 % tested. Within genomic datasets, noted plants were shared nearby water sediments, suggesting sink-source dynamic surroundings. gene analysis revealed while share common constituents environments, they enrich certain bacteria such as Mycoplasmatales, Fusobacteriales, Spirochaetales. On contrary, found viromes harbored same near equal relative abundances Our results show how could be only risk estuaries also ecosystem-level sentinels.
Language: Английский
Citations
1
iMeta, Journal Year: 2023, Volume and Issue: 2(4)
Published: Sept. 17, 2023
This study revealed that primer selection substantially influences the taxonomic and predicted functional composition characterization of microecological patterns, which was not alleviated by close-reference clustering. Biases were relatively consistent across different habitats in community profiling but patterns. These biases could be attributed to multiple aspects, including taxa specificity, regional hypervariability, amplification efficiency.
Language: Английский
Citations
18
Journal of Environmental Sciences, Journal Year: 2024, Volume and Issue: 150, P. 373 - 384
Published: Feb. 12, 2024
Language: Английский
Citations
9