Enzyme-Accelerated Catalytic DNA Circuits Enable Rapid and One-pot Detection of Bacterial Pathogens DOI
Baolin Li, Hui Jiang,

Sijian Luo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 10, 2024

Abstract Catalytic DNA circuits, serving as signal amplification strategies, can enable simple and accurate detection of pathogenic bacteria in complex matrices but suffer from low reaction rates depths. Herein, we design an enzyme-accelerated catalytic hairpin assembly (EACHA) which duplex products are converted into reactants to continue participating the next circuit with assistance RNase H. Profiting high recyclability reactants, EACHA exhibits approximately 37.6-fold enhancement rate constant a two-order-of-magnitude improvement sensitivity compared conventional (CHA). By integrating allosteric probe EACHA, one-pot method is developed for rapid direct S. enterica Enteritidis. This capable detecting 15 CFU mL− 1 S. Enteritidis within 20 min, superior that real-time PCR. testing 60 milk samples, demonstrate this method's accuracy discriminating contaminated area under curve (AUC) 0.997. Moreover, be employed accurately diagnose early-stage infected mice, AUC 1.00 feces samples 0.986 serum samples. Therefore, study offers feasible identifying pathogens matrices.

Language: Английский

DNA dendrimer-based nanocarriers for targeted Co-delivery and controlled release of multiple chemotherapeutic drugs DOI Creative Commons

Yao Tian,

Mengqiu Sun,

Rui Song

et al.

RSC Advances, Journal Year: 2025, Volume and Issue: 15(4), P. 2981 - 2987

Published: Jan. 1, 2025

DNA-based nanomaterials have attracted increasing attention over the past decades due to their incomparable programmability and functionality. In particular, dendritic DNA nanostructures are ideal for constructing drug carriers highly branched structure. this study, an intelligent delivery system was constructed based on dendrimers, in which duplexes were utilized simultaneously loading both hydrophilic hydrophobic small molecule drugs. Additionally, cancer microenvironment-responsive cell-targeting moieties introduced into internal framework surface of nanostructures, respectively. Our research shows that these can enter cells through endocytosis disintegrate under reduction cellular glutathione, thereby achieving targeted co-delivery controlled release chemotherapeutic agents antisense oligonucleotides, providing effective strategy combined treatment tumors.

Language: Английский

Citations

2
Split activator of CRISPR/Cas12a for direct and sensitive detection of microRNA DOI

Wen He,

Xinyu Li, Xinmin Li

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1303, P. 342477 - 342477

Published: March 22, 2024

Language: Английский

Citations

14
Artificial intelligence for personalized nanomedicine; from material selection to patient outcomes DOI
Hirak Mazumdar, Kamil Reza Khondakar, Suparna Das

et al.

Expert Opinion on Drug Delivery, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Applying artificial intelligence (AI) to nanomedicine has greatly increased the production of specially engineered nanoscale materials for tailored medicine, marking a significant advancement in healthcare. With use AI, researchers can search through massive databases and find nano-properties that support range therapeutic objectives, eventually producing safer, customized nanomaterials. AI analyzes patient data, including clinical genetic information, predict results individualized care makes recommendations therapy improvement. Furthermore, logically creates nanocarriers give precise controlled drug release patterns optimize advantages minimize undesirable side effects. Even though lot potential nanomedicine, there are still issues data integration techniques, moral dilemmas, requirement governmental backing. Future developments tools multidisciplinary cooperation between scientists with expertise biological sciences nanoengineering essential nanomedicine. Together, these disciplines propel advancements precision contributing ultimate objective—a future which combine provide really The authors this editorial encourage call on scientists, physicians, legislators acknowledge its transform treatment.

Language: Английский

Citations

12
Simultaneous detection of membrane protein and mRNA at single extracellular vesicle level by droplet microfluidics for cancer diagnosis DOI Creative Commons

Huixian Lin,

Bo Li,

Jingyun Guo

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Simultaneous detection of proteins and mRNA within a single extracellular vesicle (EV) enables comprehensive analysis specific EVs subpopulations, significantly advancing cancer diagnostics. However, developing sensitive user-friendly approach for simultaneously detecting multidimensional biomarkers in EV is still challenging.

Language: Английский

Citations

5
Sensitive electrochemical biosensor for rapid detection of sEV-miRNA based turbo-like localized catalytic hairpin assembly DOI

Huiqin Zhu,

Siting Chen,

Fei Lan

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1311, P. 342704 - 342704

Published: May 9, 2024

Language: Английский

Citations

4
Aptamer proximal enzyme cascade reactions for ultrafast detection of glucose in human blood serum DOI
Min Liu, Chuanlin Zhu,

Zihe Dong

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(2)

Published: Jan. 13, 2025

Language: Английский

Citations

0
Rapid and sensitive detection of exosomal microRNAs by terahertz metamaterials DOI Creative Commons
Yi Zhou, Yü Liu, Zhen Zong

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 330, P. 125745 - 125745

Published: Jan. 17, 2025

Exosomal microRNAs (miRNAs) are among the most common biomarkers for tumor diagnosis. However, single-miRNA detection lacks ideal sensitivity and specificity diagnosing a certain in clinics. In this work, we fabricated convenient multi-miRNA platform sensitive specific on exosomal miRNAs plasma of patients using terahertz (THz) metamaterial biosensor basis strand displacement amplification (SDA) AuNPs. The proposed was highly to miRNA-15, miRNA-21, miRNA-145, miRNA-155, miRNA-423, miRNA-451, limit (LOD) obtained were 12.54 aM, 19.66 17.50 25.40 24.11 aM 26.59 respectively. constructed can be used diagnose pancreatic cancer effectively complex clinical samples. use multiple demonstrated strong practicality combined diagnosis with cancer. These studies demonstrate that multi-RNA boasts advantages such as low cost, rapid, high sensitivity, specificity, offering potential tool future applications.

Language: Английский

Citations

0
Colorimetric aptasensor coupled with a deep-learning-powered smartphone app for programmed death ligand-1 expressing extracellular vesicles DOI Creative Commons
Adeel Khan, Haroon Latif Khan, Nongyue He

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 23, 2025

Lung cancer is a devastating public health threat and leading cause of cancer-related deaths. Therefore, it imperative to develop sophisticated techniques for the non-invasive detection lung cancer. Extracellular vesicles expressing programmed death ligand-1 (PD-L1) markers (PD-L1@EVs) in blood are reported be indicative response immunotherapy. Our approach development colorimetric aptasensor by combining rapid capturing efficiency (Fe 3 O 4 )-SiO 2 -TiO EV isolation with PD-L1 aptamer-triggered enzyme-linked hybridization chain reaction (HCR) signal amplification. The numerous HRPs catalyze their substrate dopamine (colorless) into polydopamine (blackish brown). Change chromaticity directly correlates concentration PD-L1@EVs sample. was able detect at concentrations as low 3.6×10 EVs/mL wide linear range from 10 high specificity successfully detected patients’ serum healthy volunteers’ serum. To transform qualitative quantitative operation, we developed an intelligent convolutional neural network (CNN)-powered analyzer form smartphone app named ExoP, thereby achieving analysis minimal user intervention or additional hardware attachments sensitive specific quantification PD-L1@EVs. This combined offers simple, sensitive, tool using addition CNN-powered further eliminates need specialized equipment, making more accessible low-resource settings.

Language: Английский

Citations

0
DNA Nanotechnology and Catalytic Hairpin Assembly Cascade: A Novel Approach in Achieving Sensitive Detection and In Situ Imaging of Phosphatidylserine on Living Cell Membranes DOI Creative Commons

Yiwei Ying,

Wenxin Qi, Zixin Chen

et al.

Sensors and Actuators Reports, Journal Year: 2025, Volume and Issue: unknown, P. 100314 - 100314

Published: March 1, 2025

Language: Английский

Citations

0
Recent advances in DNA nanotechnology for cancer detection and therapy: A review DOI

Donya Esmaeilpour,

Matineh Ghomi, Ehsan Nazarzadeh Zare‬

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142136 - 142136

Published: March 1, 2025

Language: Английский

Citations

0