Epigenetic and Cellular Reprogramming of Doxorubicin-Resistant MCF-7 Cells Treated with Curcumin DOI Open Access
Paola Poma, Salvatrice Rigogliuso,

Manuela Labbozzetta

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13416 - 13416

Published: Dec. 14, 2024

The MCF-7R breast cancer cell line, developed by treating the parental MCF-7 cells with increasing doses of doxorubicin, serves as a model for studying acquired multidrug resistance (MDR). MDR is major challenge in therapy, often driven overexpression efflux pump P-glycoprotein (P-gp) and epigenetic modifications. While many P-gp inhibitors show promise vitro, their nonspecific effects on limit vivo application. Curcumin, natural compound pleiotropic action, nontoxic inhibitor capable modulating multiple pathways. To explore curcumin’s molecular cells, we analyzed expression genes involved DNA methylation transcription regulation, including ABCB1/MDR1. Reduced representation bisulfite sequencing further unveiled key changes induced curcumin. Our findings indicate that curcumin treatment not only modulates critical cellular processes, such ribosome biogenesis cytoskeletal dynamics, but also reverses resistant phenotype, toward sensitive cells. This study highlights potential an adjuvant therapy to overcome chemoresistance, offering new avenues pharmacological strategies targeting regulation re-sensitize

Language: Английский

Epigenetic and Cellular Reprogramming of Doxorubicin-Resistant MCF-7 Cells Treated with Curcumin DOI Open Access
Paola Poma, Salvatrice Rigogliuso,

Manuela Labbozzetta

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13416 - 13416

Published: Dec. 14, 2024

The MCF-7R breast cancer cell line, developed by treating the parental MCF-7 cells with increasing doses of doxorubicin, serves as a model for studying acquired multidrug resistance (MDR). MDR is major challenge in therapy, often driven overexpression efflux pump P-glycoprotein (P-gp) and epigenetic modifications. While many P-gp inhibitors show promise vitro, their nonspecific effects on limit vivo application. Curcumin, natural compound pleiotropic action, nontoxic inhibitor capable modulating multiple pathways. To explore curcumin’s molecular cells, we analyzed expression genes involved DNA methylation transcription regulation, including ABCB1/MDR1. Reduced representation bisulfite sequencing further unveiled key changes induced curcumin. Our findings indicate that curcumin treatment not only modulates critical cellular processes, such ribosome biogenesis cytoskeletal dynamics, but also reverses resistant phenotype, toward sensitive cells. This study highlights potential an adjuvant therapy to overcome chemoresistance, offering new avenues pharmacological strategies targeting regulation re-sensitize

Language: Английский

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