Innovative Therapeutic Approach Targeting Colon Cancer Stem Cells: Transitional Cold Atmospheric Plasma DOI Creative Commons
Abolfazl Soulat, Taghi Mohsenpour, Leila Roshangar

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(12), P. 12109 - 12121

Published: March 6, 2025

Transitional cold atmospheric plasma (TCAP) represents a novel technique for generating remotely from primary source. It consists of partially nonthermal ionized gas mixture containing charged and neutral particles, photons, free radicals. In recent years, TCAP has attracted considerable attention in biomedical applications. order to evaluate colon cancer stem cells' (CCSCs) proliferation, apoptotic induction, inflammatory response, survival, was utilized both directly indirectly this study. Using argon helium gases, continuously delivered two stages during the experiment. For direct state, irradiated onto CCSCs 3 5 min. indirect technique, Matrigel treated with min before introduction cells. vitro assays demonstrated that exposure significantly reduced viability CCSCs; application had greater impacts than argon. Numerous investigations confirmed induction apoptosis, showing groups more cells altered cellular structures controls (****p < 0.0001). A substantial increase Bax/Bcl-2 ratio found by analyzing expression Bax Bcl-2 genes, indicating increased susceptibility apoptosis (*p = 0.0177 ***p 0.0004). The higher efficacy mode further highlighted marker analysis, which showed significant reduction interleukin-6 interleukin-8 TCAP-helium compared TCAP-argon (**p 0.0015 0.0007). Lastly, proliferation test, relies on Ki-67 expression, noteworthy decline all TCAP-treated groups, group exhibiting most robust impact 0.0014). Overall, findings highlight potential TCAP, particularly helium, as promising approach selectively targeting providing insights into its therapeutic mechanisms treatment. therefore, emerges unique strategy applications cell-targeted therapies.

Language: Английский

Innovative Therapeutic Approach Targeting Colon Cancer Stem Cells: Transitional Cold Atmospheric Plasma DOI Creative Commons
Abolfazl Soulat, Taghi Mohsenpour, Leila Roshangar

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(12), P. 12109 - 12121

Published: March 6, 2025

Transitional cold atmospheric plasma (TCAP) represents a novel technique for generating remotely from primary source. It consists of partially nonthermal ionized gas mixture containing charged and neutral particles, photons, free radicals. In recent years, TCAP has attracted considerable attention in biomedical applications. order to evaluate colon cancer stem cells' (CCSCs) proliferation, apoptotic induction, inflammatory response, survival, was utilized both directly indirectly this study. Using argon helium gases, continuously delivered two stages during the experiment. For direct state, irradiated onto CCSCs 3 5 min. indirect technique, Matrigel treated with min before introduction cells. vitro assays demonstrated that exposure significantly reduced viability CCSCs; application had greater impacts than argon. Numerous investigations confirmed induction apoptosis, showing groups more cells altered cellular structures controls (****p < 0.0001). A substantial increase Bax/Bcl-2 ratio found by analyzing expression Bax Bcl-2 genes, indicating increased susceptibility apoptosis (*p = 0.0177 ***p 0.0004). The higher efficacy mode further highlighted marker analysis, which showed significant reduction interleukin-6 interleukin-8 TCAP-helium compared TCAP-argon (**p 0.0015 0.0007). Lastly, proliferation test, relies on Ki-67 expression, noteworthy decline all TCAP-treated groups, group exhibiting most robust impact 0.0014). Overall, findings highlight potential TCAP, particularly helium, as promising approach selectively targeting providing insights into its therapeutic mechanisms treatment. therefore, emerges unique strategy applications cell-targeted therapies.

Language: Английский

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