Advances in multiplexed techniques for the detection and quantification of microRNAs

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 50(6), P. 4141 - 4161

Published: Jan. 1, 2021

MicroRNA detection is currently a crucial analytical chemistry challenge: almost 2000 papers were referenced in PubMed 2018 and 2019 for the keywords "miRNA method". MicroRNAs are potential biomarkers multiple diseases including cancers, neurodegenerative cardiovascular diseases. Since miRNAs stably released bodily fluids, they of prime interest development non-invasive diagnosis methods, such as liquid biopsies. Their however challenging, high levels sensitivity, specificity robustness required. The analysis also needs to be quantitative, since aim detect miRNA concentration changes. Moreover, multiplexing capability importance, clinical probably lays our ability perform parallel mapping concentrations recognize typical disease signature from this profile. A plethora biochemical innovative methods have been reported recently some them provide new solutions problem sensitive multiplex detection. In review, we propose analyze particular developments multiplexed approaches main aspects these (including sensitivity specificity) will analyzed, with focus on demonstrated each methods.

Language: Английский

---

Signaling pathways in rheumatoid arthritis: implications for targeted therapy

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: Feb. 17, 2023

Abstract Rheumatoid arthritis (RA) is an incurable systemic autoimmune disease. Disease progression leads to joint deformity and associated loss of function, which significantly impacts the quality life for sufferers adds losses in labor force. In past few decades, RA has attracted increased attention from researchers, abnormal signaling pathways are a very important research field diagnosis treatment RA, provides evidence understanding this complex disease developing novel RA-linked intervention targets. The current review intends provide comprehensive overview including general introduction disease, historical events, epidemiology, risk factors, pathological process, highlight primary progress various molecular mechanisms, genetic epigenetic summarize most recent developments identifying new inhibitors treating RA. therapeutic interventions approved drugs, clinical pre-clinical cutting-edge technologies. These will hopefully drive strategically targeted therapies hope ideas options future.

Language: Английский

---

High-Fidelity and Rapid Quantification of miRNA Combining crRNA Programmability and CRISPR/Cas13a trans-Cleavage Activity

Analytical Chemistry, Journal Year: 2019, Volume and Issue: 91(8), P. 5278 - 5285

Published: March 15, 2019

MicroRNAs (miRNAs) are short noncoding RNAs that post-transcriptionally regulate gene expression. It has been proved the aberrant expression of miRNAs is related to disease and can serve as potential biomarkers for early tumor diagnosis. The clustered regularly interspaced palindromic repeats (CRISPR)/Cas13a a recently discovered CRISPR-RNA (crRNA) guided RNA manipulation tool. recognition target morphologically activate robust nonspecific trans ribonuclease activity Cas13a. This unique property makes Cas13a ideal nucleic acid detection. Herein, we first exploited CRISPR/LbuCas13a directly detect with high specificity simplicity. A limit detection (LOD) low 4.5 amol was achieved by this one-step assay within 30 min, dynamic range spanned 4 orders magnitude from 10 100 fmol. More importantly, single nucleotide variation, even at end miRNA, be discriminated rationally programmed crRNA. In addition, practical application ability Cas13a/crRNA-based signal amplification strategy demonstrated miRNA quantification in complex biological samples (total small RNA). With excellent reliability, sensitivity, simple implement features, method promises great diagnosis miRNA-related disease. Moreover, systematic analysis crRNA design could provide guidance further develop Cas13a-based molecular diagnoses.

Language: Английский

---

Exosomes: Small vesicles with big roles in cancer, vaccine development, and therapeutics

Bioactive Materials, Journal Year: 2021, Volume and Issue: 10, P. 281 - 294

Published: Aug. 28, 2021

Cancer is a deadly disease that globally and consistently one of the leading causes mortality every year. Despite availability chemotherapy, radiotherapy, immunotherapy, surgery, cure for cancer has not been attained. Recently, exosomes have gained significant attention due to therapeutic potential their various components including proteins, lipids, nucleic acids, miRNAs, lncRNAs. Exosomes constitute set tiny extracellular vesicles with an approximate diameter 30–100 nm. They are released from different cells present in biofluids blood, cerebrospinal fluid (CSF), urine. perform crucial multifaceted functions malignant progression via autocrine, paracrine, endocrine communications. The ability carry cargoes drug molecular information recipient make them novel tool therapeutics. In this review, we discuss major role progression. We also review important literature about as vaccines delivery carriers context

Language: Английский

---

MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing

Frontiers in Microbiology, Journal Year: 2020, Volume and Issue: 11

Published: June 3, 2020

In the pursuit of improved diagnostic tests for infectious diseases, several classes molecules have been scrutinized as prospective biomarkers. Small (18-22 nucleotide), non-coding RNA transcripts called microRNAs (miRNAs) emerged promising candidates with extensive potential, due to their role in numerous previously established methods quantitation and stability within biofluids. Despite efforts identify, characterize apply miRNA signatures markers a range non-infectious application disease has advanced relatively slowly. Here, we outline benefits that biomarkers offer diagnosis, management, treatment diseases. Investigation these novel could advance use personalized medicine treatment, which raises important considerations validating or prognostic markers. Finally, discuss new emerging detection platforms, focus on rapid, point-of-care testing, evaluate obstacles disease.

Language: Английский

---

Oligodendrocyte lineage cells and depression

Molecular Psychiatry, Journal Year: 2020, Volume and Issue: 26(1), P. 103 - 117

Published: Nov. 3, 2020

Abstract Depression is a common mental illness, affecting more than 300 million people worldwide. Decades of investigation have yielded symptomatic therapies for this disabling condition but not led to consensus about its pathogenesis. There are data support several different theories causation, including the monoamine hypothesis, hypothalamic–pituitary–adrenal axis changes, inflammation and immune system alterations, abnormalities neurogenesis conducive environmental milieu. Research in these areas others has greatly advanced current understanding depression; however, there other, less widely known Oligodendrocyte lineage cells, oligodendrocyte progenitor cells mature oligodendrocytes, numerous important functions, which include forming myelin sheaths that enwrap central nervous axons, supporting axons metabolically, mediating certain forms neuroplasticity. These specialized glial been implicated psychiatric disorders such as depression. In review, we summarize recent findings shed light on how might participate pathogenesis depression, discuss new approaches targeting novel strategy treat

Language: Английский

---

miRNA-17-92 protects endothelial cells from erastin-induced ferroptosis through targeting the A20-ACSL4 axis

Biochemical and Biophysical Research Communications, Journal Year: 2019, Volume and Issue: 515(3), P. 448 - 454

Published: May 31, 2019

Language: Английский

---

Decreased miR-214–3p activates NF-κB pathway and aggravates osteoarthritis progression

EBioMedicine, Journal Year: 2021, Volume and Issue: 65, P. 103283 - 103283

Published: March 1, 2021

Osteoarthritis (OA), a disease with whole-joint damage and dysfunction, is the leading cause of disability worldwide. The progressive loss hyaline cartilage extracellular matrix (ECM) considered as its hallmark, but exact pathogenesis needs to be further clarified. MicroRNA(miRNA) contributes OA pathology may help identify novel biomarkers therapies against OA. Here we identified miR-214-3p an important regulator OA.qRT-PCR in situ hybridization were used detect expression level miR-214-3p. function OA, well interaction between downstream mRNA target (IKBKB), was evaluated by western blotting, immunofluorescence, qRT-PCR luciferase assay. Mice models introduced examine mechanism vivo.In our study, found that miR-214-3p, while being down-regulated inflamed chondrocytes cartilage, regulated ECM metabolism cell apoptosis cartilage. Mechanically, protective effect downregulated IKK-β led dysfunction NF-κB signaling pathway. Furthermore, intra-articular injection antagomir mice joints triggered spontaneous miRNA-214-3p agomir alleviated experimental mouse models.Decreased activates pathway aggravates development through targeting IKKβ, suggesting therapeutic for OA.This study financially supported grants from National Natural Science Foundation China (81,773,532, 81,974,342).

Language: Английский

---

Umbilical mesenchymal stem cell-derived exosomes facilitate spinal cord functional recovery through the miR-199a-3p/145-5p-mediated NGF/TrkA signaling pathway in rats

Stem Cell Research & Therapy, Journal Year: 2021, Volume and Issue: 12(1)

Published: Feb. 12, 2021

Abstract Background Although exosomes, as byproducts of human umbilical cord mesenchymal stem cells (hUC-MSCs), have been demonstrated to be an effective therapy for traumatic spinal injury (SCI), their mechanism action remains unclear. Methods We designed and performed this study determine whether exosomes attenuate the lesion size SCI by ameliorating neuronal induced a secondary inflammatory storm promoting neurite outgrowth. determined absolute levels all exosomal miRNAs investigated potential mechanisms miR-199a-3p/145-5p in inducing outgrowth vivo vitro. Results miR-199a-3p/145-5p, which are relatively highly expressed promoted PC12 cell differentiation suppressed lipopolysaccharide (LPS) vitro through modulation NGF/TrkA pathway. also that Cblb was direct target miR-199a-3p Cbl miR-145-5p. gene knockdown resulted significantly decreased TrkA ubiquitination levels, subsequently activating downstream pathways Akt Erk. Conversely, overexpression associated with increased level, inactivating Western blot coimmunoprecipitation assays confirmed interaction between Cbl. In experiment, found upregulate expression at site promote locomotor function rats. Conclusions summary, our showed transferring into neurons rats affected signaling pathway, indicating hUC-MSC-derived may promising treatment strategy SCI.

Language: Английский

---

The role of Exosomal miRNAs in cancer

Journal of Translational Medicine, Journal Year: 2022, Volume and Issue: 20(1)

Published: Jan. 3, 2022

Exosomal miRNAs have attracted much attention due to their critical role in regulating genes and the altered expression of virtually all cancers affecting humans (Sun et al. Mol Cancer 17(1):14, 2018). modulate processes that interfere with cancer immunity microenvironment, are significantly involved tumor growth, invasion, metastasis, angiogenesis drug resistance. Fully investigating detailed mechanism occurrence development various could help not only treatment but also prevention malignant diseases. The current review highlighted recently published advances regarding cancer-derived exosomes, e.g., sorting delivery mechanisms for RNAs. cell-to-cell communication, impacting angiogenesis, metastasis multiple biological features, were discussed. Finally, potential exosomal as diagnostic prognostic molecular markers was summarized, well usefulness detecting resistance therapeutic agents.

Language: Английский

---