LncRNA NEAT1 Promotes Vascular Endothelial Cell Dysfunction via miR-218-5p/GAB2 and Serves as a Diagnostic Biomarker for Deep Vein Thrombosis

Clinical and Applied Thrombosis/Hemostasis, Journal Year: 2023, Volume and Issue: 29

Published: Jan. 1, 2023

Deep vein thrombosis (DVT) is a common peripheral disease. This study aimed to elucidate the diagnostic biomarker of lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in DVT, and explore possible mechanisms Human umbilical endothelial cells (HUVECs).101 patients with lower extremity DVT 82 healthy controls were enrolled. RT-qPCR was designed resolve mRNA levels NEAT1, miR-218-5p, GAB2. ROC applied for diagnosis DVT. Systemic inflammation (IL-1β, IL-6, TNF-α) adhesion factor (SELP, VCAM-1, ICAM-1) examined by ELISA. And cell proliferation, migration, apoptosis conducted CCK-8, Transwell, flow cytometry assay. The targeting relationship validated Dual luciferase reporter RIP analysis.NEAT1 GAB2 upregulated while miR-218-5p decreased (P < .01). Serum NEAT1 can identify from individuals. positively correalted fibrinolysis factors, coagulation vasoconstrictors. inhibited promoted as well factors secretion HUVECs .05), but all impaired overexpression .05). expression acting sponge miR-218-5p.Elevated biomarker, implicated vascular dysfunction via miR-218-5p/GAB2 axis.

Language: Английский

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Hypothalamic Regulatory Mechanisms of Aging

Journal of Evolutionary Biochemistry and Physiology, Journal Year: 2021, Volume and Issue: 57(3), P. 473 - 491

Published: May 1, 2021

Language: Английский

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Prognostic Value of Serum Interleukin-6, NF-κB plus MCP-1 Assay in Patients with Diabetic Nephropathy

Disease Markers, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 5

Published: June 17, 2022

Objective. To assess the prognostic value of serum interleukin-6 (IL-6), nuclear factor-κB (NF-κB), and monocyte chemoattractant protein 1(MCP-1) assay in patients with diabetic nephropathy. Methods. From May 2019 to March 2020, 104 nephropathy treated our institution assessed for eligibility were recruited assigned at a ratio 1 : either observation group ([urinary albumin excretion rate (UAER)] 30 mg-300 mg/24 h) or research ([UAER] >300 h). IL-6, MCP-1, renal function indices, NF-κB levels determined, their correlation DN was analyzed. Logistic regression used analyze influencing factors end-stage disease The receiver operating characteristic (ROC) curve drawn, area under (AUC) calculated predictive combined detection prognosis Results. eligible UAER h associated significantly higher NF-κB, blood urea nitrogen (BUN), creatinine (Scr) versus those ( $P<0.05$ ). During follow-up, total 38 progressed diseases. Eligible diseases showed without id="M2"> Serum are independent risk occurrence AUCs predicting 0.562, 0.634, 0.647, respectively, AUC three 0.889. Conclusion. MCP-1 closely related injury poor nephropathy, is valuable assessing patients’ condition prognosis.

Language: Английский

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Kirenol alleviates diabetic nephropathy via regulating TGF-β/Smads and the NF-κB signal pathway

Pharmaceutical Biology, Journal Year: 2022, Volume and Issue: 60(1), P. 1690 - 1700

Published: Sept. 8, 2022

Kirenol possesses anti-inflammatory, antifibrotic and anti-arthritic effects. However, its reno-protective effects against diabetic nephropathy (DN) have not been evaluated.This study explores the of kirenol DN clarifies potential mechanisms.The mesangial cells were treated with 20 µM 10 ng/mL human recombinant TGF-β1 or 30 mM glucose for 24 h. Then harvested to assay expression target genes proteins. Thirty C57BL/6J male mice given high-fat diet streptozotocin injection induce diabetes then randomized into three groups (n = 10): vehicle administration (DM group), 2 mg/kg + group) 200 metformin (Met 3 months, orally. A healthy group (Con, n 10) was included as control.Compared DM group, treatment decreased phosphorylation Smad2/3 NF-κB (0.64- 0.43-fold) well accumulation FN Col IV (0.58- 0.35-fold); moreover, IκBα restored normal level by both in vivo vitro. After treatment, IL-6 0.35- 0.57-fold, TNF-α 0.34- 0.46-fold, vitro vivo, respectively. Furthermore, alleviated glomerular basement membrane thickness foot process fusion.Kirenol could alleviate downregulating TGF-β/Smads signal pathway. Our provides a mechanism kirenol.

Language: Английский

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DACH1, a novel target of miR-218, participates in the regulation of cell viability, apoptosis, inflammatory response, and epithelial-mesenchymal transition process in renal tubule cells treated by high-glucose

Renal Failure, Journal Year: 2020, Volume and Issue: 42(1), P. 463 - 473

Published: Jan. 1, 2020

Objective This report was designed to assess the functional role of miR-218/dachshund family transcription factor 1 (DACH1) in diabetic kidney disease (DKD) and investigate its possible molecular mechanism.Materials Methods From GEO database, we downloaded different datasets for analyzing expression miR-218 DACH1 DKD. TargetScan adopted predict binding sites between DACH1, which further verified by dual-luciferase reporter assays. The renal proximal tubule cells (HK-2) treated with high glucose (HG) were used as an vitro model. QRT-PCR western blot determine other relative factors. Cell counting kit-8 flow cytometer applied detect cell viability apoptosis. levels inflammatory cytokines determined ELISA assay.Results A prominent raise observed DKD through bioinformatics analysis, confirmed HG-induced is a target miR-218. reduced induced apoptosis negatively regulating DACH1. Moreover, upregulating HG models increased concentrations pro-inflammatory TNF-α IL-1β, level anti-inflammatory cytokine IL-10, promoted epithelial-mesenchymal transition (EMT) process, possibly achieved targeting While downregulating showed opposite results.Conclusion These data demonstrated that, under environment, suppressed HK-2 proliferation, apoptosis, caused response, facilitated EMT process largely providing insight into therapeutic intervention

Language: Английский

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