Patient-Specific and Regional Factors Influencing Proliferation Dynamics of Adult Human Neural Stem/Progenitor Cells from Spinal Cord Tissue DOI Creative Commons
Sasi Kumar Jagadeesan, Ryan Sandarage, Mitesh Patel

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Abstract Direct comparison of proliferation characteristics between primary adult human neural stem/progenitor cells (NSPCs) and their animal counterparts has been challenging due to the difficulty in obtaining viable spinal cord tissue. Here, we present first assessment rates NSPCs derived from samples development a multivariate linear regression model predict dynamics. We cultured 42 tissue samples, sourced 27 organ donors, examine doubling times rates. Key characteristics, including sex, age, region (thoracic, lumbar, conus), neurological conditions (e.g., epilepsy, Parkinson's disease, schizophrenia), substance use (alcohol misuse, intravenous drug use, polydrug use) were analyzed for correlation with NSPC proliferative behavior. Immunocytochemical analysis confirmed multipotency these NSPCs, indicating capability differentiate into multiple lineages. A was developed rates, encoding categorical variables as integers using binary values 7-fold cross-validation ensuring robustness. The demonstrated high predictive performance (MSE = 0.006335, MAE 0.0217, R² 0.887), validation on an independent cohort yielded 0.916 MSE 0.018003. Our findings revealed significant decline older individuals those histories, while conus exhibited higher Furthermore, conditions, such epilepsy found correlate reduced highlighting impact underlying pathology stem cell

Language: Английский

Mechanisms of radiation‐induced tissue damage and response DOI Creative Commons

Zhou Lin,

Jiaojiao Zhu, Yuhao Liu

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 20, 2024

Radiation-induced tissue injury (RITI) is the most common complication in clinical tumor radiotherapy. Due to heterogeneity response of different tissues radiation (IR), radiotherapy will cause types and degrees RITI, which greatly limits application Efforts are continuously ongoing elucidate molecular mechanism RITI develop corresponding prevention treatment drugs for RITI. Single-cell sequencing (Sc-seq) has emerged as a powerful tool uncovering mechanisms identifying potential targets by enhancing our understanding complex intercellular relationships, facilitating identification novel cell phenotypes, allowing assessment spatiotemporal developmental trajectories. Based on comprehensive review we analyzed regulatory networks combination with Sc-seq summarized targeted intervention pathways therapeutic Deciphering diverse underlying can shed light its pathogenesis unveil new avenues potentially facilitate repair or regeneration currently irreversible Furthermore, discuss how personalized strategies based offer promise mitigating

Language: Английский

Citations

5
Modeling Radiation-Induced Epithelial Cell Injury in Murine Three-Dimensional Esophageal Organoids DOI Creative Commons

Latisha Carswell,

Deepa Sridharan,

Lung-Chang Chien

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(5), P. 519 - 519

Published: April 25, 2024

Esophageal squamous cell carcinoma (ESCC) is a deadly consequence of radiation exposure to the esophagus. ESCC arises from esophageal epithelial cells that undergo malignant transformation and features perturbed differentiation program. Understanding dose- quality-dependence epithelium response may provide insights into ability promote ESCC. We have explored factors play role in radiosensitivity their potential relationship risk. utilized murine three-dimensional (3D) organoid model recapitulates morphology functions stratified esophagus study persistent quality-dependent changes. Interestingly, although high-linear energy transfer (LET) Fe ion induced more intense alteration 53BP1 DNA damage foci levels as compared Cs, MAPK/SAPK stress pathway signaling showed similar altered for most phospho-proteins with both qualities. In addition, lower dose high-LET also revealed nearly same degree morphological changes, even though only ~36% were predicted be hit at 0.1 Gy dose, suggesting bystander effect induced. Although p38 ERK/MAPK highest following exposure, findings reveal low (0.1 Gy) qualities can elicit critically impact gradient epithelium, providing novel pathogenesis radiation-induced injury early stage carcinogenesis.

Language: Английский

Citations

0
Patient-Specific and Regional Factors Influencing Proliferation Dynamics of Adult Human Neural Stem/Progenitor Cells from Spinal Cord Tissue DOI Creative Commons
Sasi Kumar Jagadeesan, Ryan Sandarage, Mitesh Patel

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Abstract Direct comparison of proliferation characteristics between primary adult human neural stem/progenitor cells (NSPCs) and their animal counterparts has been challenging due to the difficulty in obtaining viable spinal cord tissue. Here, we present first assessment rates NSPCs derived from samples development a multivariate linear regression model predict dynamics. We cultured 42 tissue samples, sourced 27 organ donors, examine doubling times rates. Key characteristics, including sex, age, region (thoracic, lumbar, conus), neurological conditions (e.g., epilepsy, Parkinson's disease, schizophrenia), substance use (alcohol misuse, intravenous drug use, polydrug use) were analyzed for correlation with NSPC proliferative behavior. Immunocytochemical analysis confirmed multipotency these NSPCs, indicating capability differentiate into multiple lineages. A was developed rates, encoding categorical variables as integers using binary values 7-fold cross-validation ensuring robustness. The demonstrated high predictive performance (MSE = 0.006335, MAE 0.0217, R² 0.887), validation on an independent cohort yielded 0.916 MSE 0.018003. Our findings revealed significant decline older individuals those histories, while conus exhibited higher Furthermore, conditions, such epilepsy found correlate reduced highlighting impact underlying pathology stem cell

Language: Английский

Citations

0