Single-Nucleus RNA Sequencing Reveals Cellular Transcriptome Features at Different Growth Stages in Porcine Skeletal Muscle

Cells, Journal Year: 2025, Volume and Issue: 14(1), P. 37 - 37

Published: Jan. 2, 2025

Porcine latissimus dorsi muscle (LDM) is a crucial source of pork products. Meat quality indicators, such as the proportion fibers and intramuscular fat (IMF) deposition, vary during growth development pigs. Numerous studies have highlighted heterogeneous nature skeletal muscle, with phenotypic differences reflecting variations in cellular composition transcriptional profiles. This study investigates cellular-level characteristics LDM large white pigs at two stages (170 days vs. 245 days) using single-nucleus RNA sequencing (snRNA-seq). We identified 56,072 cells across 12 clusters, including myofibers, fibro/adipogenic progenitor (FAP) cells, satellite (MUSCs), other resident cell types. The same types were present both stages, but their proportions states differed. A higher FAPs was observed 245-day-old Additionally, these exhibited more active communication compared to 170-day-old For instance, interactions found between pericytes or endothelial pigs, collagen integrin family signaling. Three subclasses identified, comprising FAPs_COL3A1+, FAPs_PDE4D+, FAPs_EBF1+, while adipocytes categorized into Ad_PDE4D+ Ad_DGAT2+ subclasses. differed age groups. also constructed differentiation trajectories for adipocytes, revealing that differentiated toward fibrosis, characteristic reminiscent high prevalence fibrosis aging humans. Furthermore, adipocyte subclass, predominant originated from FAPs_PDE4D+ expressing gene, subclass stemmed FAPs_EBF1+. In conclusion, our elucidates provides insights mechanisms relevant meat diseases.

Language: Английский

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Single‐cell RNA‐seq reveals novel interaction between muscle satellite cells and fibro‐adipogenic progenitors mediated with FGF7 signalling

Journal of Cachexia Sarcopenia and Muscle, Journal Year: 2024, Volume and Issue: 15(4), P. 1388 - 1403

Published: May 16, 2024

Abstract Background Muscle satellite cells (MuSCs) exert essential roles in skeletal muscle adaptation to growth, injury and ageing, their functions are extensively modulated by microenvironmental factors. However, the current knowledge about interaction of MuSCs with niche is quite limited. Methods A 10× single‐cell RNA sequencing (scRNA‐seq) was performed on porcine longissimus dorsi soleus (SOL) muscles generate a transcriptomic dataset myogenic other cell types. Sophisticated bioinformatic analyses, including unsupervised clustering analysis, marker gene, gene set variation analysis (GSVA), AUCell, pseudotime velocity were explore heterogeneity cells. CellChat used demonstrate cell–cell communications across subpopulations cells, especially fibro‐adipogenic progenitors (FAPs). Integrated human mice datasets verify expression FGF7 diverse species. The role MuSC proliferation evaluated through administering recombinant MuSCs, C2C12, cardiotoxin (CTX)‐injured d ‐galactose ( ‐gal)‐induced ageing model. Results ScRNA‐seq totally figured out five types myo‐lineage FAPs, further classified into six subpopulations, termed as RCN3 + , S100A4 ID3 cycling (MKI67 ), MYF6 MYMK respectively. There higher proportion c ycling SOL population. uncovered particular impact FAPs mediated FGF7, which relatively highly expressed samples. Administration (10 ng/mL) significantly increased EdU C2C12 4.03 ± 0.81% P < 0.01) 6.87 2.17% 0.05), respectively, knockdown FGFR2 dramatically abolished pro‐proliferating effects 0.05). In CTX‐injured muscle, ratio /Pax7 15.68 5.45% 0.05) elevated number eMyHC regenerating myofibres 19.7 4.25% 0.01). Under ‐gal stimuli, reduced γH2AX 17.19 3.05% induced 4.34 1.54% restored myofibre size loss running exhaustion vivo (all Conclusions Our scRNA‐seq reveals novel between FGF7–FGFR2. Exogenous augments thus benefits regeneration counteracts age‐related myopathy.

Language: Английский

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The Regulatory Role of CircAGGF1 in Myogenic Differentiation and Skeletal Muscle Development

Animals, Journal Year: 2025, Volume and Issue: 15(5), P. 708 - 708

Published: Feb. 28, 2025

Circular RNA (circRNA) has a significant impact on the maturation of skeletal muscle, although their precise functions within this framework remain largely uncharted. This study presents an investigation regulatory effect circAGGF1 myogenesis in myoblasts, including potential molecular mechanisms involved. It is revealed that facilitates differentiation myoblasts into other states while simultaneously enhancing manifestation type I muscle fibers. In vivo investigations with mice promotion expansion and by circAGGF1, bolstering its regenerative capacity. Mechanistically, interacts miR-199a-3p acting as sponge, promoting subsequent expression Fgf7. Furthermore, rescue experiments indicated counteraction induced overexpression miR-199a-3p. To summarize, research highlights role played development providing valuable resource for our understanding biology.

Language: Английский

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Deciphering the role of IGFBP5 in delaying fibrosis and sarcopenia in aging skeletal muscle: therapeutic implications and molecular mechanisms

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 12, 2025

Introduction Sarcopenia is a condition characterized by the loss of muscle fibers and excessive deposition extracellular matrix proteins. The interplay between atrophy fibrosis central feature sarcopenia. While mechanisms underlying skeletal aging remain incompletely understood, cellular senescence has emerged as key contributor. This study investigates role D-galactose (D-gal) in inducing fibroblasts fibrosis, aims to find regulator process serve therapeutical target. Methods To discover D-gal markers expression fibrosis-related proteins were assessed after introducing among fibroblasts, strength mass. severity also verified using H&amp;E staining Masson trichrome treatment via subcutaneous injection mice. Subsequently, mRNA sequencing (RNA-seq) was performed differential expressed genes identified under or control treatment, D-GAL-driven fibrosis. IGFBP5 then validated D-GAL treated IGFBP5-knockdown vitro analyzing level markers. And results further confirmed vivo SAMP8 mice with histological examinations. Results effectively induced well atrophy, mass function RNA-seq. validation tests showed that could alleviate D-GAL-induced fibroblast Discussion emerging findings provide new insights into molecular age-related highlight potential therapeutic Further research needed validate these explore related clinical applications.

Language: Английский

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Streptococcus salivarius subsp. thermophilus ST-G30 Prevents Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes

Nutrients, Journal Year: 2025, Volume and Issue: 17(7), P. 1141 - 1141

Published: March 26, 2025

Background/Objectives: Sarcopenia is characterized by loss of muscle mass and strength associated with aging. Recently, its links the gut-muscle axis have been reported, suggesting that probiotics could influence health. Methods: In present study, we investigated protective roles two lactic acid bacteria strains, Streptococcus salivarius subsp. thermophilus ST-G30 (ST-G30) Lacticaseibacillus paracasei LPc-G110 (LPc-G110), on skeletal atrophy induced dexamethasone (DEX) in C2C12 myotubes. Results: Our results demonstrated significantly alleviated DEX-induced myotube increasing myotubes' diameter (25.95 ± 1.28 vs. 15.30 0.30 μm, p < 0.01), improving fusion index (48.35 1.75 22.16 2.36%, 0.0001), protein content (1.78 0.02 1.56 0.01 mg/mL, 0.05) length (0.61 0.05 0.33 0.01, 0.05), whereas showed no significant effect these phenotypes (p > 0.05). Transcriptomic analysis reveals modulates critical signaling pathways biological processes related to current KEGG enrichment WGCNA enabled identification PI3K-Akt pathway as a key regulator processes, highlighting essential role mitigating atrophy. Furthermore, overlapping DEGs strong correlations atrophy-related indices. Conclusions: These findings underscore potential promising anti-muscle supplement provide valuable insights for developing strategies prevent treat glucocorticoid-induced

Language: Английский

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Roles of Insulin-Like Growth Factor-1 in Muscle Wasting and Osteopenia in Mice with Hyponatremia

Calcified Tissue International, Journal Year: 2025, Volume and Issue: 116(1)

Published: April 14, 2025

Abstract Hyponatremia is associated with sarcopenia and osteoporosis in elderly individuals. Skeletal muscle releases myokines, which affect distant organs, including bone. However, the detailed mechanisms by hyponatremia influences bone remain unclear. We herein investigated effects of on muscle, bone, myokines linking to mice treated 1-desamino-8-D-arginine vasopressin (dDAVP) or furosemide, induce hyponatremia. Muscle mass mineral density (BMD) were analyzed 8 weeks after administration dDAVP furosemide. significantly reduced grip strength, but did not tissue weights gastrocnemius soleus muscles mice. Furosemide decreased mass, muscles, strength furosemide trabecular BMD, volume, cortical BMD at femurs. Among expression insulin-like growth factor (IGF)-1 serum IGF-1 levels In simple regression analyses, positively related expression, The sodium chloride solution ameliorated decreases circulating furosemide-treated present study demonstrated that induces loss as well a decrease findings suggest might be wasting induced

Language: Английский

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Key genes and processes affected by atorvastatin treatment in mouse diaphragm muscle

Archives of Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Language: Английский

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Modulation of senescent Lepr+ skeletal stem cells via suppression of leptin-induced STAT3‒FGF7 axis activation alleviates abnormal subchondral bone remodeling and osteoarthritis progression

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 5, 2025

Recent studies have suggested that targeting senescent cells in joint tissues may alleviate osteoarthritis (OA) progression. However, this strategy encounters significant challenges, partially due to the high degree of cellular heterogeneity osteoarthritic tissues. Moreover, little information is available on role skeletal stem cell (SSC) senescence, as compared differentiated cells, OA In study, single-cell RNA sequencing (scRNA-seq) articular cartilages and subchondral bones knee joints mice with post-traumatic (PTOA) were performed. Further vivo vitro performed reveal mechanisims SSCs during development lesions progression by microCT, pathological analysis, functional gain loss experiments. The one-way ANOVA was used multiple group data analysis. scRNA-seq demonstrated leptin receptors (Lepr) positive underwent senescence addition, leptin-Lepr signaling pathway induced signal transducer activator transcription 3 (STAT3) expression SSCs, which consequently augmented fibroblast growth factor 7 (FGF7). analyses revealed FGF7 exacerbated abnormal bone remodeling enhancing formation suppressing resorption. analysis osteogenic differentiation but inhibited osteoclastogenesis a concentration-dependent manner. summary, our findings demonstrate promotes SSC exacerbates activating STAT3-FGF7 axis progression, shed light novel therapeutic strategies for OA.

Language: Английский

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Muscle-homing peptides modified biomimetic curcumin nanoparticles ameliorate skeletal muscle dysfunction in aging mice

Redox Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103679 - 103679

Published: May 1, 2025

Language: Английский

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Innovative Insights into Single-Cell Technologies and Multi-Omics Integration in Livestock and Poultry

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12940 - 12940

Published: Dec. 2, 2024

In recent years, single-cell RNA sequencing (scRNA-seq) has marked significant strides in livestock and poultry research, especially when integrated with multi-omics approaches. These advancements provide a nuanced view into complex regulatory networks cellular dynamics. This review outlines the application of scRNA-seq key species, including poultry, swine, ruminants, focus on outcomes related to heterogeneity, developmental biology, reproductive mechanisms. We emphasize synergistic power combining epigenomic, proteomic, spatial transcriptomic data, enhancing molecular breeding precision, optimizing health management strategies, refining production traits poultry. The integration these technologies offers multidimensional approach that not only broadens scope data analysis but also provides actionable insights for improving animal productivity.

Language: Английский

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