SYISL Knockout Promotes Embryonic Muscle Development of Offspring by Modulating Maternal Gut Microbiota and Fetal Myogenic Cell Dynamics DOI Creative Commons
Hao Zuo, Wei Jiang, Jianwei Gao

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Embryonic muscle fiber formation determines post-birth totals. The previous research shows SYISL knockout significantly increases numbers and mass in mice, but the mechanism remains unclear. This study confirms that gene, maternal gut microbiota, their interaction affect number of fibers mouse embryos through distinct mechanisms, as alters microbiota composition boosts butyrate levels embryonic serum. Both fecal transplantation feeding increase offspring, with inhibiting histone deacetylases increasing acetylation muscle. Combined analysis RNA-seq between wild-type mice ChIP-seq for H3K9ac H3K27ac reveals regulates myogenesis via butyrate-HDAC-H3K9ac/H3K27ac pathway. Furthermore, scRNA-seq alone proportion myogenic cells dynamics, independently regulating levels. Cell communication suggests this may be due to downregulation signaling pathways such MSTN TGFβ. Overall, multiple are highlighted which influences development, offering valuable insights treating diseases improving livestock production.

Language: Английский

The maternal lifestyle in pregnancy: Implications for foetal skeletal muscle development DOI Creative Commons

Haijun Sun,

Meixia Chen,

Jialong Liao

et al.

Journal of Cachexia Sarcopenia and Muscle, Journal Year: 2024, Volume and Issue: 15(5), P. 1641 - 1650

Published: Aug. 18, 2024

The world is facing a global nutrition crisis, as evidenced by the rising incidence of metabolic disorders such obesity, insulin resistance and chronic inflammation. Skeletal muscle largest tissue in humans plays an important role movement host metabolism. Muscle fibre formation occurs mainly during embryonic stage. Therefore, maternal lifestyle, especially exercise pregnancy, has critical influence on foetal skeletal development subsequent health offspring. In this review, malnutrition micronutrient intake systematically summarized. We also aim to describe how shapes gut microbiota its metabolites further discussed, although field still 'infancy'. This review will provide new insights reduce crisis highlight current gaps promote research.

Language: Английский

Citations

4
Balancing LncRNA H19 and miR‐675 Bioconversion as a Key Regulator of Embryonic Myogenesis Under Maternal Obesity DOI Creative Commons
Yao Gao, Md Nazmul Hossain, Liang Zhao

et al.

Journal of Cachexia Sarcopenia and Muscle, Journal Year: 2025, Volume and Issue: 16(2)

Published: March 31, 2025

ABSTRACT Background Maternal obesity (MO) impairs fetal skeletal muscle development, but the underlying mechanisms remain poorly defined. The regulatory roles of lncRNA H19 and its first exon derived microRNA675 ( miR675 ) in prenatal development to be examined. / Igf2 are same imprinting cluster with expressed from maternal allele while expresses paternally. contains a G‐rich loop, KH‐type splicing protein (KHSRP) mediates biogenesis pre‐ miRNAs containing loops, which depends on phosphorylation by AKT, key mediator IGF2 signalling. This study aims depict elusive function these regulators that affected MO during embryonic myogenesis. Methods Single‐cell transcriptomic sequencing GeoMx spatial RNA were performed identify differentially genes between embryos control (CT) mice. Both E11.5 E13.5 collected analysed validate data. miR657 myogenesis further P19 cells via CRISPR/dCas9‐mediated activation inhibition. epigenetic changes methylated DNA immunoprecipitation, allele‐targeted analysis was crossing C57BL/6J CAST/EiJ Results Transcriptomic showed contained less differentiated myocytes (1.34%) than CT (2.86%). Myogenesis‐related GO biological processes down‐regulated myotome region. lower expression myogenic transcription factors such as Myf5 , Myod1 Myog Mef2c Myh3 p < 0.05). altered modifications genomic cluster, showing decreased methylation level region 0.05) diallelic pattern elevated embryos. Overexpression inhibited cells, promoted myogenesis, suggesting critical bioconversion . A KHSRP process relies IGF2/AKT Knocking‐down inhibition AKT abolished biogenesis. suppressed signalling blocked KHSRP‐dependent Conclusions We found differential effects up‐regulates blocks suppressing IGF2/AKT/KHSRP axis. Myogenesis impeded due highly accumulated

Language: Английский

Citations

0
Restoration of vascular dysfunction resulting from maternal high-fat diet via modulation of the NLRP3/IL-1β axis DOI Creative Commons
Yuxuan Xiao, Xiaoning Bi, Rongjie Zhang

et al.

Clinical and Experimental Hypertension, Journal Year: 2024, Volume and Issue: 47(1)

Published: Dec. 26, 2024

This study investigated the impact of maternal high-fat diet on vascular function and endothelial homeostasis in offspring. We found that offspring exposed to exhibited elevated blood pressure, impaired abdominal aortic function, imbalance. These changes were accompanied by increased levels reactive oxygen species (ROS) upregulation pro-inflammatory cytokines (including IL-1β, TNF-α, IL-6, IL-10). Treatment with NLRP3 or IL-1β inhibitors prevented deterioration reduced NO production, inflammation induced exposure compared control group. The findings suggest during pregnancy, mitigating impairments can be achieved inhibiting NLRP3/IL-1β pathway.

Language: Английский

Citations

2
SYISL Knockout Promotes Embryonic Muscle Development of Offspring by Modulating Maternal Gut Microbiota and Fetal Myogenic Cell Dynamics DOI Creative Commons
Hao Zuo, Wei Jiang, Jianwei Gao

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Embryonic muscle fiber formation determines post-birth totals. The previous research shows SYISL knockout significantly increases numbers and mass in mice, but the mechanism remains unclear. This study confirms that gene, maternal gut microbiota, their interaction affect number of fibers mouse embryos through distinct mechanisms, as alters microbiota composition boosts butyrate levels embryonic serum. Both fecal transplantation feeding increase offspring, with inhibiting histone deacetylases increasing acetylation muscle. Combined analysis RNA-seq between wild-type mice ChIP-seq for H3K9ac H3K27ac reveals regulates myogenesis via butyrate-HDAC-H3K9ac/H3K27ac pathway. Furthermore, scRNA-seq alone proportion myogenic cells dynamics, independently regulating levels. Cell communication suggests this may be due to downregulation signaling pathways such MSTN TGFβ. Overall, multiple are highlighted which influences development, offering valuable insights treating diseases improving livestock production.

Language: Английский

Citations

0