Chemo‐taxonomic and biological potential of highly therapeutic plantPedicularis groenlandicaRetz. using multiple microscopic techniques

Microscopy Research and Technique, Journal Year: 2021, Volume and Issue: 84(12), P. 2890 - 2905

Published: June 2, 2021

Abstract Pedicularis groenlandica is one of the most important medicinal plant Deosai Plateau (Gilgit‐Baltistan) Pakistan. The present study was aim to evaluate micromorphological features, phytochemical screening and pharmacological potential P. by using multiple microscopic techniques. Six different solvents were used prepare extracts. Phytochemical antioxidant activities determined calorimetrically. To investigate antidiabetic, α‐amylase inhibition assay performed. Cytotoxicity tested brine shrimp assay. Anti‐leishmanial via MTT Disc‐diffusion for protein kinase inhibitory, antibacterial antifungal activities. Palyno‐anatomical showed significant variation authentication correct identification this highly therapeutic light scanning electron All extracts found rich in phytochemicals, amount phenolic flavonoid contents methanol extract (PGM) 95.78 mg GAE/g 66.90 QE/g. Highest DPPH scavenging with IC 50 88.65 μg/mL, total capacity (60.33 AAE/g sample) reducing power (83.97 AAE/g) PGM. method Noticeable growth L. tropica displayed n‐hexane (IC 112 μg/mL). Brine highest LD (67.65 μg/mL) ethyl‐acetate extract. Ethanol gives persuasive (26 mm) against Streptomyces 85‐E hyphae. alpha‐amylase (74.10%) In conclusion, our findings scientifically support ethno‐medicinal biological . future, needs be explored further isolation bioactive compounds develop new drugs treat several aliments.

Language: Английский

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Silene conoidea L.: A Review on its Systematic, Ethnobotany and Phytochemical profile

Plant Science Today, Journal Year: 2019, Volume and Issue: 6(4), P. 373 - 382

Published: Oct. 1, 2019

Silene conoidea L. is a weed commonly grown in subtropical and temperate zone of western Eurasia, some part Himalaya. S. small herb height from 20 to 40cm mostly grow as different crops like wheat, barley, mustard, roquette lentil pea. This the first attempt gather utmost information about species distribution, taxonomy, ethno-medicines, phytochemical analysis, biological activity etc. conoidea. The present review aim was collect fragmented addition explore its therapeutic importance future research opportunities. A total 100 articles were reviewed using sources like; Scopus, web science, Science direct, google scholar PubMed. are used multipurpose activities against microbes, bacteria insecticidal. also important due high contents various chemicals compounds. Chemical extracts contain flavones, glycosides, saponins, sterols glycosides. study shows that can be an indicator gold. Traditional values for treating respiratory, antioxidant, anti-diabetic anticancer needed validated through more research. Ethno-pharmacological clinical trial subjected studies.

Language: Английский

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Morphological characterization of Hypnaceae (Bryopsida, Hypnales): Investigating four genera from Western Himalayas by using LM and SEM techniques

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 83(6), P. 676 - 690

Published: Feb. 16, 2020

Abstract The Hypnaceae is one of the largest and diversified family among pleurocarpous mosses which consists nearly 60 genera 1,000 species around world. In Pakistan, it represented by 15 8 genera. current research article provides detail morphological investigation four different (genera) that is, Gollania clarescens , Hypnum revolutum Homomallium andoi Taxiphyllum taxirameum in Western Himalayas, Pakistan. reports first SEM study leaf surfaces studied taxa, along with comprehensive characters species. main objective project to present comparative light scanning electron microscopic discuss morphology because previously just reported bryophyte checklists Himalayas. Based on results, characters, micromorphological observations, qualitative quantitative attributes, taxonomic keys for distribution, habitat are described. Stereoscope was used analyze microscope observe measure laminal alar cells leaf. reveals many important surface features like cell shape wall. were mostly linear elongated thin walls. specimens air dried, so somewhat constricted concaved. will help make contribution taxonomy this family.

Language: Английский

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Scanning electron microscopic identification of ten novel, non‐edible oil seeds for bioenergy production

Microscopy Research and Technique, Journal Year: 2022, Volume and Issue: 85(9), P. 3245 - 3255

Published: June 28, 2022

Abstract Biodiesel is a sustainable, inexpensive, and alternative energy source produced from vegetable oils animal fats. Precise authentic identification of oil yielding plant species very crucial. Therefore, scanning electron microscopy (SEM) was employed in our current investigation to study micromorphological characteristics ten novel seeds for their reliable identification. Macromorphological characters sample were explored by light microscopy. Seed size varied 16 6.2 mm length 18.4–4.5 width. shape ovoid cordial color beige brown. content ranged 25% 45% (w/w). Whereas free fatty acid (FFA) seed 0.42 3.4 mg KOH/g. potential Chamaerops humilis found be highest (98%) all. Besides, ultra‐structural observation demonstrated variation surface sculpturing which rugous, reticulate, perforate, striate, webby. Periclinal wall arrangements rough, ridged, depressed, thick curved whereas, anticlinal walls pattern showed wavy, smooth, raised, deep depressed. It ultimately concluded that Scanning could serve as an advanced tool representing hidden seeds. offers significant knowledge researchers local community accurate genuine Research Highlights Non‐edible promising bioenergy. Variation Micromorphological among selected Classification non‐edible plants via characters.

Language: Английский

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Palyno‐anatomical microscopic characterization of selected species of Boraginaceae and Fabaceae

Microscopy Research and Technique, Journal Year: 2021, Volume and Issue: 85(4), P. 1332 - 1354

Published: Dec. 25, 2021

Palyno-anatomical characterization of some selected species Boraginaceae and Fabaceae using light microscopy (LM) scanning electron (SEM) was conducted. Six them belongs to four genera family other six Fabaceae. The current study aimed investigate palyno-anatomical features for correct identification taxonomic significance. Pollen grains were acetolyzed observed both qualitatively quantitatively through microscopy. Statistical data analyzed cluster analysis which categorized plants into clusters. Principal component (PCA) carried out illustrate morphological variation among plant species. Studied showed in shape, size, aperture type, exine ornamentation pollen, size shape epidermal cells, types stomata, guard subsidiary cells trichome types. Exine thickness recorded maximum 2.30 ± 0.1 μm Lablab purpureus minimum 1.05 0.2 Gastrocotyle hispida. PCA summarized 73.78% accumulative variance. Trichome index highest 62% on the adaxial surface Heliotropium europaeum lowest 21% L. purpureus. These findings reinforce importance taxa. It concluded that description characteristics presented this study, highly contribute our knowledge

Language: Английский

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Comparative pollen and foliar micromorphological studies using light microscopy and scanning electron microscopy of some selected species of Lamiaceae from Alpine Zone of Deosai Plateau, Western Himalayas

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 83(6), P. 579 - 588

Published: Jan. 31, 2020

Abstract The study was conducted to highlight a detailed account of morphology pollen chosen species Lamiaceae through scanning electron microscopy, and the anatomical characteristics leaf epidermis seven using simple light microscopy. In results, Anisomeles indica Otostegia aucheri belong subfamily Lamioideae because it has tricolpate while rest eight Nepetoideae (hexacolpate pollen). exine sculpturing studied found be reticulate. family Lamiaceae, four kinds stomata were anomocytic, anisocytic, diacytic, actinocytic, respectively. cell wall patterns epidermal cells irregular or polygonal with straight undulate walls. It noted that variety trichomes seems taxonomically important for identification Lamiaceae. Both nonglandular glandular analyzed. characterized long, thin, pointed apical unicellular cells. A‐shaped in Thymus linearis . Perovskia abrotanoides , stellate observed whereas A. Mentha royleana both found. sessile peltate M. For first time this study, foliar micromorphological features selected area are carried out. These taxonomic characters discrimination from each other. future, comprehensive coupled other is required delimitation taxa at various levels.

Language: Английский

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Microscopic techniques used for the identification of medicinal plants: A case study of Senna

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 82(10), P. 1660 - 1667

Published: July 3, 2019

The use of quality control tool for adulteration Senna (Cassia aungustifolia) a pharmaceutically very important. They were used multiple health disorders such as constipation, indigestion, epilepsy, asthma, piles, migraine, and heart problems. Two different species same family or genus commercially in Indo-Pak using the medicine name Senna. One named (C. its adulterant Sickle obtusifolia).These two plants analyzed classical microscopic techniques light microscopy modern chemotaxonomic traits scanning electron microscopy, fluorescence studies phytochemical studies.The C. aungustifolia L. had found to be perennial herb with trilobed pollen, diacytic, paracytic, anisocytic stomata having smooth walled epidermal cells, whereas obtusifolia stands out shrub spheroidal circular pollen paracytic type irregular shaped cells. powdered drug is dark grayish green, green color. Investigation other this project provided basis authentication species.

Language: Английский

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Leaf and stem micromorphology of Byrsonima sericea DC. by light and scanning electron microscopy

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(3), P. 287 - 296

Published: Nov. 14, 2019

Micromorphological studies were carried out using multiple microscopic techniques on the leaves and stem bark of Byrsonima sericea DC. (Malpighiaceae), a species popularly known as "murici" used medicinally, in order to identify both qualitative quantitative features leaf anatomy histochemistry differential parameters support quality control its ethnodrugs taxonomy genus. The study was conducted traditional plant anatomy, histochemical tests, stomatal index (SI). has hypostomatic leaves, anomocytic stomata, epidermal walls are anticlinal straight adaxial curved abaxial faces. T-shaped trichomes observed mainly surface. epidermis showed waxes syntopism surfaces, with occurrence different crystalloid forms single phylloplane. mesophyll is dorsiventral, 3-4 collateral vascular bundles. Phenolic compounds, starch, proteins identified petiole stem. SI 14.5 ± 0.53% (p < .05), but did not significant variations. A set characters found be distinctive for studied species, however, constituting that could separate B. from other

Language: Английский

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Foliar epidermal anatomy of some selected wild edible fruits of Pakistan using light microscopy and scanning electron microscopy

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(3), P. 259 - 267

Published: Nov. 12, 2019

Abstract The present study is focused on the detailed foliar epidermal anatomy of some selected wild edible fruits (WEFs) from Pakistan using light microscopy (LM) and scanning electron (SEM). studied species are Ficus racemosa L., Solanum nigrum Capparis spinosa Physalis divaricata D.Don, Rosa moschata Herrm. Ribes orientale Desf. collected various localities Pakistan. objective to investigate qualitative quantitative anatomical characters for identification differentiation fruits. shape size cells, anticlinal wall pattern, trichome type shape, average number stomata, length width stomata pore. microscopic investigation variations in recorded have a key role determination authentication This possesses great potential plant taxonomists further evaluate at molecular genetic levels.

Language: Английский

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Micromorphological Foliar Screening for Identification of Moraceae Taxa using Light Microscopy (LM) and Scanning Electron Microscopy (SEM) Techniques

Polish Journal of Environmental Studies, Journal Year: 2024, Volume and Issue: 33(4), P. 4141 - 4152

Published: Feb. 16, 2024

A study on foliar epidermis was conducted nine species belonging to the Moraceae family investigate diverse micromorphological characteristics that hold taxonomic importance in leaf epidermis.Based LM (light microscopy) and SEM (scanning electron microscopy), it found shape of epidermal cells studied members is rectangular, irregular, polygonal, pentagonal both surfaces.The common polygonal most species.The size cell length width varies from wall thick species, except Broussonetia papyrifera (L.) L'Hér.ex Vent.and Morus alba L., laevigata Wall.ex Brandis, where are thin surfaces.It majority plants hypostomatic, i.e., adaxial surface leaves has no stomata.Most have anomocytic stomata abaxial leaf.The cyclocytic were only Ficus elastica Roxb.ex Hornem.and benjamina L. The type, which rare cyclocytic, laterocytic, paracytic.We variations surfaces all selected important element stomatal index, serves as a geographic indicator shows transpiration rate index ranges (76.7%) B. (2.13%) F. surface, while highest (61.8%) religiosa lowest (3.06%) virens Aiton.The identification at genus specific-levels be taxonomically appropriate based architecture leaves.Thus, current aims clarify qualitative quantitative properties order give information for categorization.

Language: Английский

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