Evaluation of chromium phytoremediation potential of some plant species of Dir Lower, Khyber Pakhtunkhwa, Pakistan

Acta Ecologica Sinica, Journal Year: 2019, Volume and Issue: 40(2), P. 158 - 165

Published: Dec. 19, 2019

Language: Английский

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“Phytochemical and antimicrobial investigation of methanolic extract/fraction of Ocimum basilicum L.”

Biocatalysis and Agricultural Biotechnology, Journal Year: 2020, Volume and Issue: 31, P. 101894 - 101894

Published: Dec. 16, 2020

Language: Английский

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Foliar epidermal micromorphology and its taxonomic significance in Polygonatum (Asparagaceae) using scanning electron microscopy

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 83(11), P. 1381 - 1390

Published: July 12, 2020

Abstract In the current investigation, 16 samples of six species genus Polygonatum were collected from different geographical regions in China. Leaf micromorphological traits studied detail using scanning electron microscopy. About five‐leaf used for each regions. A significant variation was found foliar epidermal such as stomata types and their distribution, cell shape, anticlinal wall pattern, various trichomes. Polygonal cells observed P. cyrtonema , odoratum sibiricum filipes rectangular P . zanlanscianense irregular shape Straight pattern most species, but straight to little undulate Similarly, that are, oval, oblong, elliptical is, paracytic, anomocytic, actinocytic have been observed. Based on traits, closely related one another. On other hand, involucratum shows a strong association However, showed very less similarity with five species. Hence this study adds useful information may be helpful further taxonomic classification taxa at subspecies level correct identification discrimination

Language: Английский

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Pollen morphology of family Solanaceae and its taxonomic significance

Anais da Academia Brasileira de Ciências, Journal Year: 2020, Volume and Issue: 92(3)

Published: Jan. 1, 2020

The pollen micro-morphology of family Solanaceae from the different phytogeographical region Pakistan has been assessed. In this study, thirteen species belonging to ten genera have studied using light and scanning electron microscopy for both qualitative quantitative features. is a eurypalynous significant variation was observed in size, shape, polarity exine sculpturing. Examined plant includes, Brugmansia suaveolens, Capsicum annuum, Cestrum parqui, Datura innoxia, Solanum lycopersicum, Nicotiana plumbaginifolia, Petunia hybrida, Physalis minima, americanum, erianthum, melongena, surattense Withania somnifera. prominent type tricolporate shed as monad. High fertility reflects that taxa are well-known study area. Based on traits taxonomic key developed accurate quick identification species. Principal Component Analysis performed shows some morphological features main characters identification. Cluster separate cluster. findings highlight importance Palyno-morphological characterization Solanaceous taxa. It concluded LM SEM significantly play role correct studied.

Language: Английский

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Palynological characteristics of selected Lamioideae taxa and its taxonomic significance

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 84(3), P. 471 - 479

Published: Sept. 21, 2020

Abstract Lamioideae comprised the second‐largest subfamily in Lamiaceae. Although considerable progress has recently been made taxonomic study of Lamioideae, remains one most poorly investigated Therefore, present was designed with aim to document pollen micromorphology some selected taxa and its significance from Pakistan. Pollen micromorphological features were observed using scanning electron microscopy. The grains are monad, tricolpate, radially/bilateral symmetrical. small medium‐sized having oblate, oblate/subspheroidal, subspheroidal shape. Exine sculpturing as reticulate, microreticulate, bireticulate. colpus surface ornamentation found verrucate, gemmate, scabrate, psilate. There a variation between species micromorphology, that is, coarseness reticulum, thickness muri comprising reticulum number secondary lumina per primary lumen. Hence, this documented morphology Pakistan strengthens identification based on characters, which helps correct identification, discrimination at generic level.

Language: Английский

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Taxonomic implication of leaf epidermal anatomy of selected taxa of Scrophulariaceae from Pakistan

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 84(3), P. 521 - 530

Published: Sept. 29, 2020

Abstract The family Scrophulariaceae consists of taxonomically complex genera and species. delimitation the taxa within this is always challenging. In paper, we studied leaf epidermis anatomical characteristics its taxonomic significance four species belonging to collected from northern Pakistan. were examined under light scanning electron microscopes (LM SEM). Qualitative quantitative foliar epidermal features for both adaxial abaxial surfaces. characters like cell shape, cover, anticlinal wall, trichomes type, stomata type position examined. Quantitative length width epidermis, stomata, stomatal pore, subsidiary surfaces measured. Stomatal index between was found be different on Diacytic glandular only in Anticharis glandulosa while rest has anomocytic dendroid Based micromorphological characters, did principal component analysis (PCA), cluster identification. A key been provided delimit identify based characters. aim present research elucidate distinguish purposes.

Language: Английский

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Palynomorphological diversity among the Asteraceous honeybee flora: An aid to the correct taxonomic identification using multiple microscopic techniques

Microscopy Research and Technique, Journal Year: 2021, Volume and Issue: 85(2), P. 570 - 590

Published: Sept. 18, 2021

This study aimed to characterize the palynological morphology of melliferous species family Asteraceae belonged seven tribes which were categorized into 15 genera and studied under light scanning electron microscopy. The pollen grains acetolyzed, measured, described qualitatively. Quantitative data analyzed by descriptive multivariate statistical analysis. considering 11 quantitative characteristics. revealed that are circular, triangular, angular, semi-angular, quadrangular, pentagonal hexagonal amb, 3-colporate or 3-colpate. size is variable among species, ranged from 56.1 23.2 μm. Three types: oblate spheroidal (eight species), prolate (six suboblate (two species) observed. surface pattern exine varies echinoperforate, echinate, echinate microperforate, fenestrate, echinoperforate reticulate, microreticulate, perforate, tectate, scabrate. Exine thickness was calculated minimum in Cichorium intybus (2.47 μm) maximum Taraxacum campylodes (5.15 μm). A key bee floral based on morphological features grains, also provided. palynomorphological characters here can be potentially used correctly identify Asteraceous honeybee most commonly plants visited honeybees area.

Language: Английский

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Pollen micromorphology of eastern Chinese Polygonatum and its role in taxonomy by using scanning electron microscopy

Microscopy Research and Technique, Journal Year: 2021, Volume and Issue: 84(7), P. 1451 - 1461

Published: Feb. 13, 2021

Abstract Although considerable progress has recently been made in the taxonomic study of Asparagaceae, but Polygonatum remains one most poorly investigated genus. Therefore, present was designed with aim, to document pollen micromorphology closely related taxa and its significance. Pollen micro‐morphological features were observed using scanning electron microscopy—SEM). The grains are monad, navicular, monocolpate, radially symmetrical. A significant variation exine sculpturing. Moreover, have perforate while some them mixed psilate perforate, microreticulate, sometimes scabrate, gemmate baculate. Hence, this documented morphology strengthens identification genus based on characters, which helps can be used as an additional tool for correct discrimination species at generic level.

Language: Английский

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Pollen morphology of selected melliferous plants and its taxonomic implications using microscopy

Microscopy Research and Technique, Journal Year: 2022, Volume and Issue: 85(7), P. 2361 - 2380

Published: March 5, 2022

This research aimed to explore the melliferous bee flora pollen from arid zones of District Attock, Islamabad Capital and Rawalpindi. Morpho-palynological features has provided key information for classification help explain taxonomy several genera species. Therefore, in this work, we examined morphological characters potentially be used species delimitation flora. A total 18 floral species, classified into 12 different families were collected, pressed, identified then acetolyized observe grains under microscopy. Bee can described by small, medium large sized morphotypes presenting five types shapes, prolate spheroidal (seven species), sub-prolate (six oblate (three (one each) was examined. The observed polar equatorial diameter found maximum Zea mays 73.5 μm Pelargonium inquinans 66.75 respectively. Pollen six namely: tricolporate, 3-zonocolporate, monoportae, tetracolporate, tetraporate mononsulcate. Whereas highest value colpi length measured Grewia tenax (24.55 μm). Exine surface nature echinate psilate scabrate. thickness noted Verbena tenuisecta (8.40 μm) minimum Citrus macrocarpa (0.4 considered difficult identify based on other traits successfully distinguished using quantitative qualitative traits, confirming importance morphology diagnose correctly honeybee RESEARCH HIGHLIGHTS: diversity among Highly variations via microscopy micromorphology. Palyno-morph taxonomic identification

Language: Английский

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Feasible regeneration and agro bacterium-mediated transformation of Brassica juncea with Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene

Saudi Journal of Biological Sciences, Journal Year: 2020, Volume and Issue: 27(5), P. 1324 - 1332

Published: Jan. 8, 2020

In the present study an effort has been made to optimize in vitro regeneration protocol for Agrobacterium-mediated transformation of Brassica juncea, because its importance as oilseed crops. The highest callus induction frequency 87% was observed on MS (Murashige and Skoog, 1962) medium supplemented with 4 µM 6-benzyladenine (BA) after four weeks culture period. Subculturing organogenic calli media a similar hormonal composition resulted shoot organogenesis six cultivation. (92%) recorded containing BA combination 1 α-naphthalene acetic acid (NAA). Further, well-developed roots were formed augmented 6 Indole (IAA) Kinetin (Kn). Cotyledon explants exploited successful B. juncea. A binary vector comprised Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene under transcriptional control glycinin promoter basta selection marker introduced into A. tumefaciens strain GV3101 via electroporation. EaDAcT is responsible unusual triacylglycerol's production where sn-3 position esterified acetate instead long-chain fatty found triacylglycerol's. (100%) transgenic shoots plus NAA presence 25 mg l-1 160 timintin. efficiency stable be approximately 7% plants. Moreover, transformed regenerated confirmed by PCR analysis using gene-specific primers.

Language: Английский

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