Extracellular vesicle analytical science loses a touch of creativity and kindness

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(9)

Published: Sept. 1, 2024

Language: Английский

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Extracellular vesicles of different cellular origin feature distinct biomolecular corona dynamics

Nanoscale Horizons, Journal Year: 2024, Volume and Issue: 10(1), P. 104 - 112

Published: Nov. 7, 2024

Initially observed on synthetic nanoparticles, the existence of biomolecular corona and its role in determining nanoparticle identity function are now beginning to be acknowledged biogenic particularly extracellular vesicles - membrane-enclosed shuttling proteins, nucleic acids, metabolites which released by cells for physiological pathological communication we developed a methodology based fluorescence correlation spectroscopy track formation derived from human red blood amniotic membrane mesenchymal stromal when these dispersed plasma. The allows tracking dynamics

Language: Английский

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Recent Advancements in Imaging Techniques for Individual Extracellular Vesicles

Molecules, Journal Year: 2024, Volume and Issue: 29(24), P. 5828 - 5828

Published: Dec. 10, 2024

Extracellular vesicles (EVs), secreted from most cells, are small lipid membranes of 30 to 1000 nm in diameter and contain nucleic acids, proteins, intracellular organelles originating donor cells. EVs play pivotal roles intercellular communication, particularly forming niches for cancer cell metastasis. However, derived cells exhibit significant heterogeneity, complicating the investigation EV subtypes using ensemble averaging methods. In this context, we highlight recent studies that characterize individual advanced techniques, including single-fluorescent-particle tracking, single-metal-nanoparticle single-non-label-particle super-resolution microscopy, atomic force microscopy. These techniques have facilitated high-throughput analyses properties particles such as their sizes, compositions, physical properties. Finally, address challenges need be resolved via single-particle (-molecule) imaging microscopy future research.

Language: Английский

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Addressing Heterogeneity in direct analysis of Extracellular Vesicles and analogues using Membrane-Sensing Peptides as Pan-Affinity Probes

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 20, 2023

Abstract Extracellular vesicles (EVs), crucial mediators of cell-to-cell communication, hold immense potential for diagnostic applications due to their ability enrich protein biomarkers in body fluids. However, challenges isolating EVs from complex biological specimens hinder widespread use. In this frame, integrated isolation-and-analysis workflows are the go-to strategy, most which see prevalence immunoaffinity methods. Yet, high heterogeneity poses challenges, as proposed ubiquitous markers less homogenously prevalent than believed, raising concerns about reliability downstream biomarker discovery programs. This issue extends burgeoning field engineered EV-mimetics and bio-nanoparticles, where conventional immune-affinity methods may lack applicability. Addressing these we introduce use Membrane Sensing Peptides (MSP) “universal” affinity ligands both EV-analogues. Employing a streamlined process integrating on-bead capture vesicle phenotyping through Single Molecule Array (SiMoA) technology, showcase application MSP analysis circulating blood derivatives, eliminating need prior EV isolation. Demonstrating possible clinical translation directly detect an EV-associated epitope signature serum plasma samples, demonstrating its distinguishing patients with myocardial infarction versus stable angina. At last, notably, exhibits unique capability enable tetraspanin-lacking Red Blood Cell derived (RBC-EVs). Overall, unlike traditional antibody-based methods, probes work agnostically, overcoming limitations associated surface abundance or scarcity. highlights advancing diagnostics beyond. Of note, represents also first-ever peptide-based SiMoA technology.

Language: Английский

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Biogenic nanoparticles from liquid and solid matrices: biochemical and biophysical properties of Extracellular Vesicles-enriched samples from human plasma and skeletal muscle tissue.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 19, 2024

The majority of studies on extracellular vesicles (EVs) focused samples isolated from liquid matrices, such as cell culture media and blood, due to their accessibility. However, recent research highlights the emerging roles EVs derived solid tissues, including brain, muscles tumors. Investigating matrix tissues offers insights into microenvironment potential biological influences surrounding cells. This study presents a method for comparing EV-enriched (human skeletal muscle biopsy) (plasma) addressing technical challenges minimizing biases in separation techniques. By employing optimized protocols advanced analytical techniques, reveals differences biochemical composition, nanomechanical properties, particle yield, size distribution, colloidal stability between human plasma EVs. Understanding these distinctions may contribute development novel diagnostic assays muscular pathologies shed light EVs' diverse tissue environments.

Language: Английский

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