Multi-Omics Profiles of Small Intestine Organoids in Reaction to Breast Milk and Different Infant Formula Preparations DOI Open Access
Xianli Wang, Shangzhi Yang, Chengdong Zheng

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(17), P. 2951 - 2951

Published: Sept. 2, 2024

Ensuring optimal infant nutrition is crucial for the health and development of children. Many infants aged 0-6 months are fed with formula rather than breast milk. Research on cancer cell lines animal models limited to examining effects milk, as it does not comprehensively consider absorption, metabolism, social determinants its physiology. Our study utilized small intestine organoids induced from human embryo stem (ESC) compare nutritional milk five donors during their postpartum lactation period 1-6 three types Stage 1 formulae regular retail stores. Using transcriptomics untargeted metabolomics approaches, we focused differences such growth development, junctions, extracellular matrix. We also analyzed roles pathways including AMPK, Hippo, Wnt, identified key genes ALPI, SMAD3, TJP1, WWTR1 development. Through observational in-vitro analysis, our demonstrates ESC-derived might be a promising model exploring underlying mechanisms.

Language: Английский

Gut–X axis DOI Creative Commons
Lin Xu, Yu Zhang, Xueyan Li

et al.

iMeta, Journal Year: 2025, Volume and Issue: 4(1)

Published: Feb. 1, 2025

Abstract Recent advances in understanding the modulatory functions of gut and microbiota on human diseases facilitated our focused attention contribution to pathophysiological alterations many extraintestinal organs, including liver, heart, brain, lungs, kidneys, bone, skin, reproductive, endocrine systems. In this review, we applied “gut–X axis” concept describe linkages between other organs discussed latest findings related axis,” underlying mechanisms potential clinical intervention strategies.

Language: Английский

Citations

2
Neonatal enteroids absorb extracellular vesicles from human milk‐fed infant digestive fluid DOI Creative Commons
Claire K. Yung, Yang Zhang, Madeline Kuhn

et al.

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(4)

Published: April 1, 2024

Abstract Human milk contains extracellular vesicles (HMEVs). Pre‐clinical models suggest that HMEVs may enhance intestinal function and limit inflammation; however, it is unknown if or their cargo survive neonatal human digestion. This limits the ability to leverage HMEV as additives infant nutrition therapeutics. study aimed develop an EV isolation pipeline from small volumes of contents after feeding (digesta) address hypothesis in vivo digestion be taken up epithelial cells (IECs). Digesta was collected nasoduodenal sampling tubes ostomies. EVs were isolated raw pasteurized digesta by density‐gradient ultracentrifugation following two‐step skimming, acid precipitation caseins, multi‐step filtration. validated electron microscopy, western blotting, nanoparticle tracking analysis, resistive pulse sensing, super‐resolution microscopy. uptake tested enteroids. (dEVs) show typical morphology are enriched CD81 CD9, but depleted β‐casein lactalbumin. some dEV fractions contain mammary gland‐derived protein BTN1A1. Neonatal enteroids rapidly take dEVs part via clathrin‐mediated endocytosis. Our data can digestive fluid these absorbed IECs.

Language: Английский

Citations

7
Mechanistic insight into human milk extracellular vesicle‐intestinal barrier interactions DOI Creative Commons

Xiang Luo,

Yunyue Zhang, Ning Ding

et al.

Journal of Extracellular Biology, Journal Year: 2025, Volume and Issue: 4(1)

Published: Jan. 1, 2025

Abstract Human milk extracellular vesicles (EVs) are crucial mother‐to‐baby messengers that transfer biological signals. These EVs reported to survive digestion and transport across the intestine. The mechanisms of interaction between human intestinal mucosa, including epithelial uptake remain unclear. Here, we studied with gut barrier components, biofluids, enzymes, mucus epithelium. Additionally, probed endocytic mediating EV uptake. Finally, using proteomic analysis, determined existence identification proteins enriched in fraction transported We show largely stable biochemical barriers demonstrate high diffusivity. a level cell (∼70%) efficient Caco‐2 monolayers. Whilst was mediated by multiple routes, none pathway‐specific inhibitors inhibited their translocation. Proteomic analysis monolayers identified 14 may facilitate transport. findings significantly expand our understanding interactions barriers,

Language: Английский

Citations

0
Microscopic messengers: Extracellular vesicles shaping gastrointestinal health and disease DOI Creative Commons
Zhantao Yu,

Kevin Swift,

Madeline A. Hedges

et al.

Physiological Reports, Journal Year: 2025, Volume and Issue: 13(7)

Published: April 1, 2025

Abstract The field of extracellular vesicles (EVs) is advancing rapidly, and this review aims to synthesize the latest research connected EVs gastrointestinal tract. We will address new emerging roles for derived from internal sources such as pancreas immune system how these miniature messengers alter organismal health or inflammatory response within GI examine what known about external dietary bacterial immense anti‐inflammatory, immune‐modulatory, proliferative potential nano‐sized information carriers. EV interactions with intestinal colonic epithelium associated cells at homeostatic disease states, necrotizing enterocolitis (NEC) bowel (IBD) also be covered. discuss are being leveraged therapeutics drug delivery conclude a series unanswered questions in field.

Language: Английский

Citations

0
Extracellular vesicles: From large-scale production and engineering to clinical applications DOI Creative Commons

Yuxuan Wang,

Jiali Xiong,

Kun Ouyang

et al.

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

Extracellular vesicles (EVs) have emerged as a promising strategy for treating wide spectrum of pathologies, they can deliver their cargo to recipient cells and regulate the signaling pathway these modulate fate. Despite great potential EVs in clinical applications, low yield challenges loading remain significant obstacles, hindering transition from experimental research practice. Therefore, promoting EV release enhancing cargo-loading are fields with substantial broad application prospects. In this review, we summarize applications EVs, methods technologies large-scale production, engineering, modification, well that must be addressed during development. We also discuss future perspectives exciting field facilitate its transformation bench reality.

Language: Английский

Citations

0
Milk Extracellular Vesicles: A Promising Oral Drug Delivery System for Intestinal Diseases DOI
Yuxuan Wang,

Kun Ouyang,

Yunxin Liao

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 61, P. 104641 - 104641

Published: June 26, 2024

Language: Английский

Citations

1
Overcoming drug resistance through extracellular vesicle-based drug delivery system in cancer treatment DOI Open Access
Long Tai Zheng,

R. S. F. Chang,

B. Liang

et al.

Cancer Drug Resistance, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Drug resistance is a major challenge in cancer therapy that often leads to treatment failure and disease relapse. Despite advancements chemotherapeutic agents targeted therapies, cancers develop drug resistance, making these treatments ineffective. Extracellular vesicles (EVs) have gained attention for their potential applications delivery because of natural origin, biocompatibility, ability cross biological barriers. Using the unique properties EVs could enhance accumulation at target sites, minimize systemic toxicity, precisely specific cells. Here, we discuss characteristics functionalization EVs, mechanisms engineered overcome resistance. This review provides comprehensive overview EV-based systems overcoming We highlight revolutionize offer promising strategies more effective modalities.

Language: Английский

Citations

1
Multi-Omics Profiles of Small Intestine Organoids in Reaction to Breast Milk and Different Infant Formula Preparations DOI Open Access
Xianli Wang, Shangzhi Yang, Chengdong Zheng

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(17), P. 2951 - 2951

Published: Sept. 2, 2024

Ensuring optimal infant nutrition is crucial for the health and development of children. Many infants aged 0-6 months are fed with formula rather than breast milk. Research on cancer cell lines animal models limited to examining effects milk, as it does not comprehensively consider absorption, metabolism, social determinants its physiology. Our study utilized small intestine organoids induced from human embryo stem (ESC) compare nutritional milk five donors during their postpartum lactation period 1-6 three types Stage 1 formulae regular retail stores. Using transcriptomics untargeted metabolomics approaches, we focused differences such growth development, junctions, extracellular matrix. We also analyzed roles pathways including AMPK, Hippo, Wnt, identified key genes ALPI, SMAD3, TJP1, WWTR1 development. Through observational in-vitro analysis, our demonstrates ESC-derived might be a promising model exploring underlying mechanisms.

Language: Английский

Citations

0